# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# S100A10 / TRP channels

- Basic Information
- External Database
- PPI pairs (4)
- Biological Process (3)
- Disease (0)

Name: Protein S100-A10 (S100A10)
Official Symbol: S100A10 provided by HGNC
Function:
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 6281 | P60903 |  | P60903 | MINT-1178035 | 112189 | P60903 |  | hsa:6281 | 114085
Mouse | 20194 | P08207 | DIP-49529N | P08207 | MINT-4133605 | 203049 | P08207 |  | mmu:20194 |
Rat | 81778 | P05943 | DIP-46650N | P05943 | MINT-4586198 | 249650 | P05943 |  | rno:81778 |

PPI pairs:

TRPC6 | TRPM3 | TRPV5 | TRPV6

Biological Process:

- GO:0071229 : cellular response to acid
- GO:0051099 : positive regulation of binding
- GO:0007165 : signal transduction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC6 |  | S100A10 | Inference |  |  |  |  |  | Prediction | 23671622
TRPM3 |  | S100A10 | Inference |  |  |  |  |  | Prediction | 22451665
TRPV5 |  | S100A10 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 12660155
TRPV6 |  | S100A10 | Inference |  |  |  |  |  | Prediction | 12660155
TRPV6 |  | S100A10 | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified |  | 16189514

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC6 |  | S100A10 | Fluorescence probe labeling | Rat | 801-878 | E. coli | Human | Full-length | E. coli | 23671622
TRPM3 |  | S100A10 | Fluorescence probe labeling | Human | 35-124 | E. coli | Human | Full-length | E. coli | 22451665
TRPM3 |  | S100A10 | Fluorescence probe labeling | Human | 291-382 | E. coli | Human | Full-length | E. coli | 22451665
TRPV5 |  | S100A10 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 12660155
TRPV6 |  | S100A10 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 12660155

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | S100A10 | Co-immunofluorescence staining | Rat duodenum tissue |  |  |  |  |  | 12660155
TRPV6 |  | S100A10 | Co-immunofluorescence staining | Rat duodenum tissue |  |  |  |  |  | 12660155
TRPV6 |  | S100A10 | Co-immunoprecipitation | Caco-2 cell |  |  |  |  |  | 18187190
TRPV6 |  | S100A10 | Co-immunoprecipitation | Human 16HBE14o- cell |  |  |  |  |  | 18187190

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC6 |  | S100A10 | Fluorescence probe labeling | Rat | 801-878 | Human | Not determined |  | 310 nM | 23671622
TRPM3 |  | S100A10 | Fluorescence probe labeling | Human | 291-382 | Human | Not determined |  |  | 22451665
TRPM3 |  | S100A10 | Fluorescence probe labeling | Human | 35-124 | Human | Not determined |  |  | 22451665
TRPV5 |  | S100A10 | Fusion protein-pull down assay | Mouse | 591-595 | Mouse | Not determined |  |  | 12660155
TRPV6 |  | S100A10 | Fusion protein-pull down assay | Mouse | 597-601 | Mouse | Not determined |  |  | 12660155

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV5 |  | S100A10 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 12660155
TRPV5 |  | S100A10 | Patch clamp |  |  | Activation | 12660155
TRPV6 |  | S100A10 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 12660155
TRPV6 |  | S100A10 | Patch clamp |  |  | Activation | 12660155

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.