# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# FYN / TRP channels

- Basic Information
- External Database
- PPI pairs (4)
- Biological Process (26)
- Disease (0)

Name: Tyrosine-protein kinase Fyn (FYN)
Official Symbol: FYN provided by HGNC
Function:
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 2534 | P06241 | DIP-33876N | P06241 | MINT-93594 | 108810 | P06241 |  | hsa:2534 | 137025
Mouse | 14360 | P39688 | DIP-198N | P39688 | MINT-85422 | 199773 | P39688 |  | mmu:14360 |
Rat | 25150 | Q62844 | DIP-47815N |  | UNK-5463756 | 247215 | Q62844 |  | rno:25150 |

PPI pairs:

TRPC4 | TRPC6 | TRPV4 | TRPV6

Biological Process:

- GO:0050798 : activated T cell proliferation
- GO:0007411 : axon guidance
- GO:0006816 : calcium ion transport
- GO:0071363 : cellular response to growth factor stimulus
- GO:0071375 : cellular response to peptide hormone stimulus
- GO:0048813 : dendrite morphogenesis
- GO:0050966 : detection of mechanical stimulus involved in sensory perception of pain
- GO:0007631 : feeding behavior
- GO:0030900 : forebrain development
- GO:0007243 : intracellular protein kinase cascade
- GO:0007612 : learning
- GO:0050900 : leukocyte migration
- GO:0010629 : negative regulation of gene expression
- GO:0042177 : negative regulation of protein catabolic process
- GO:0001764 : neuron migration
- GO:0018108 : peptidyl-tyrosine phosphorylation
- GO:0030168 : platelet activation
- GO:0046777 : protein autophosphorylation
- GO:0008360 : regulation of cell shape
- GO:0050690 : regulation of defense response to virus by virus
- GO:0042493 : response to drug
- GO:0045471 : response to ethanol
- GO:0031295 : T cell costimulation
- GO:0050852 : T cell receptor signaling pathway
- GO:0016032 : viral reproduction
- GO:0019048 : virus-host interaction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC4 |  | FYN | Inference |  |  |  |  |  | Prediction | 16144838
TRPC6 |  | FYN | Inference |  |  |  |  |  | Prediction | 14761972
TRPV4 |  | FYN | Inference |  |  |  |  |  | Prediction | 12538589
TRPV6 |  | FYN | Inference |  |  |  |  |  | Prediction | 17197020

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC6 |  | FYN | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Human | 149-257 | E. coli | 14761972

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | FYN | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 18048348
TRPC4 |  | FYN | Co-immunoprecipitation | COS-7 |  |  |  |  |  | 16144838
TRPC4 |  | FYN | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC6 |  | FYN | Co-immunofluorescence staining |  | CHO-K1 | Mouse | Full-length | Human | Full-length | 14761972
TRPC6 |  | FYN | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 14761972
TRPC6 |  | FYN | Co-immunoprecipitation |  | COS-7 | Mouse | Full-length | Human | Full-length | 14761972
TRPV4 |  | FYN | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 12538589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC6 |  | FYN | Fusion protein-pull down assay | Mouse | 1-388 | Human | 149-257 |  |  | 14761972
TRPV4 |  | FYN | Co-immunoprecipitation | Mouse | Not determined | Mouse | 145-247 |  |  | 12538589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC6 |  | FYN | In vivo PTM assay | Phosphorylation |  |  | 14761972
TRPC6 |  | FYN | Patch clamp |  |  | Activation | 14761972
TRPV4 |  | FYN | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | FYN | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589
TRPV6 |  | FYN | In vitro PTM assay | Phosphorylation |  |  | 17197020

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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