# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# SNAPIN / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (10)
- Disease (0)

Name: SNARE-associated protein Snapin (SNAPIN)
Official Symbol: SNAPIN provided by HGNC
Function:
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 23557 | O95295 | DIP-31359N | O95295 | MINT-5002303 | 117101 | O95295 |  | hsa:23557 | 607007
Mouse | 20615 | Q9Z266 | DIP-44321N | Q9Z266 | MINT-3389684 | 203363 | Q9Z266 |  | mmu:20615 |
Rat | 295217 | P60192 | DIP-37034N | P60192 | MINT-3389957 | 254916 | P60192 |  | rno:295217 |

PPI pairs:

TRPC6 | TRPM7 | TRPV1

Biological Process:

- GO:0008089 : anterograde axon cargo transport
- GO:0048490 : anterograde synaptic vesicle transport
- GO:0006886 : intracellular protein transport
- GO:0032438 : melanosome organization
- GO:0031175 : neuron projection development
- GO:0016079 : synaptic vesicle exocytosis
- GO:0031629 : synaptic vesicle fusion to presynaptic membrane
- GO:0016188 : synaptic vesicle maturation
- GO:0072553 : terminal button organization
- GO:0019048 : virus-host interaction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC6 |  | SNAPIN | Inference |  |  |  |  |  | Prediction | 17684020
TRPM7 |  | SNAPIN | Yeast two-hybrid | Human | 87-326 | Human | Brain | cDNA library |  | 17088214
TRPV1 |  | SNAPIN | Yeast two-hybrid | Rat | 1-414 | Rat | Brain | cDNA library |  | 15066994
TRPV1 |  | SNAPIN | Yeast two-hybrid | Rat | 1-432 | Human | Fetal brain | cDNA library |  | 17074976

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM7 |  | SNAPIN | Fusion protein-pull down assay | Human | 83-326 | In vitro translation | Human | Full-length | Not specified | 17088214
TRPV1 |  | SNAPIN | Fusion protein-pull down assay | Rat | 1-414 | E. coli | Human | Full-length | In vitro translation | 15066994

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC6 |  | SNAPIN | Co-immunoprecipitation | Rat brain cortex |  |  |  |  |  | 17684020
TRPM7 |  | SNAPIN | Co-immunoprecipitation | Rat brain synaptic vesicle |  |  |  |  |  | 17088214
TRPV1 |  | SNAPIN | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 15066994

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM7 |  | SNAPIN | Fusion protein-pull down assay | Human | 87-326 | Human | 43-68 |  |  | 17088214

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.