# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPML1 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (33)
- Biological Process (3)
- Disease (1)

Name: Mucolipin-1 (TRPML1)
Official Symbol: MCOLN1 provided by HGNC
Function:
Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 57192 | Q9GZU1 |  | Q9GZU1 | MINT-1409368 | 121441 | Q9GZU1 | 501 | hsa:57192 | 605248
Mouse | 94178 | Q99J21 |  |  |  | 220458 | Q99J21 |  | mmu:94178 |
Rat | 288371 | D3ZRF9 |  |  |  | 252540 |  |  | rno:288371 |

PPI pairs:

ALG-2/PDCD6 | Cdc42 | ERIGC3 | HOP/STI1 | HSC70 | HSP40/DNAJB1 | HSP40/DNAJC14 | HSP90
LAPTM4a | LAPTM4b | LAPTM5 | NDKA | NovelProtein/NP9 | PEA-15 | PEX16 | PIP5K1A
PKA | Peroxisomal membrane protein 2 | RFP/TRIM27 | Rac1 | Rac2 | RhoG | SEC13 | SEC31A
SLC35E1 | STOML1 | TMEM163 | TPC1 | TPC2 | TRPML2 | TRPML3 | Unnamed protein (BAE)
YIF1B

Biological Process:

- GO:0006816 : calcium ion transport
- GO:0006879 : cellular iron ion homeostasis
- GO:0033572 : transferrin transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:605248 : Mucolipidosis IV (OMIM:252650 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPML1 |  | ALG-2/PDCD6 | Affinity purification-mass spectrometry | Human | 1-66 |  |  | HeLa lysates |  | 19864416
TRPML1 |  | Cdc42 | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | ERIGC3 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Mouse | Heart | cDNA library |  | 23418601
TRPML1 |  | HOP/STI1 | Inference |  |  |  |  |  | Prediction | 19117012
TRPML1 |  | HSC70 | Yeast two-hybrid | Human | 94-298 | Human | Brain | cDNA library |  | 19117012
TRPML1 |  | HSP40/DNAJB1 | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | HSP40/DNAJC14 | Yeast two-hybrid | Human | 94-298 | Human | Brain | cDNA library |  | 19117012
TRPML1 |  | HSP90 | Inference |  |  |  |  |  | Prediction | 19117012
TRPML1 |  | LAPTM4a | Yeast two-hybrid | Human | Full-length | Human | adult brain | cDNA library |  | 21224396
TRPML1 |  | LAPTM4b | Yeast two-hybrid | Human | Full-length | Human | adult brain | cDNA library |  | 21224396
TRPML1 |  | LAPTM5 | Speculation |  |  |  |  |  | Prediction | 21224396
TRPML1 |  | NDKA | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | NovelProtein/NP9 | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | PEA-15 | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | Peroxisomal membrane protein 2 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Mouse | Heart | cDNA library |  | 23418601
TRPML1 |  | PEX16 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Mouse | Heart | cDNA library |  | 23418601
TRPML1 |  | PIP5K1A | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Mouse | Heart | cDNA library |  | 23418601
TRPML1 |  | PKA | Inference |  |  |  |  |  | Prediction | 17988215
TRPML1 |  | Rac1 | Speculation |  |  |  |  |  | Prediction | 23418601
TRPML1 |  | Rac2 | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | RFP/TRIM27 | Yeast two-hybrid | Human | Full-length | Human | Fetal brain | cDNA library |  | 16169070
TRPML1 |  | RhoG | Speculation |  |  |  |  |  | Prediction | 23418601
TRPML1 |  | SEC13 | Affinity purification-mass spectrometry | Human | 1-66 |  |  | HeLa lysates |  | 19864416
TRPML1 |  | SEC31A | Affinity purification-mass spectrometry | Human | 1-66 |  |  | HeLa lysates |  | 19864416
TRPML1 |  | SLC35E1 | Yeast two-hybrid | Human | Full-length | Human | Fetal brain | cDNA library |  | 16169070
TRPML1 |  | STOML1 | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | RAW264.7 macrophages | Lysates |  | 23418601
TRPML1 |  | TMEM163 | Yeast two-hybrid | Mouse | Full-length | Mouse | Kidney | cDNA library |  | 25130899
TRPML1 |  | TPC1 | Inference |  |  |  |  |  | Prediction | 21540176
TRPML1 |  | TPC2 | Inference |  |  |  |  |  | Prediction | 21540176
TRPML1 |  | TRPML2 | Inference |  |  |  |  |  | Prediction | 16606612
TRPML1 |  | TRPML3 | Inference |  |  |  |  |  | Prediction | 16606612
TRPML1 |  | Unnamed protein (BAE) | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Mouse | Heart | cDNA library |  | 23418601
TRPML1 |  | YIF1B | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Mouse | Heart | cDNA library |  | 23418601

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPML1 |  | ALG-2/PDCD6 | Fusion protein-pull down assay | Human | 1-66 | E. coli | Human | Full-length | HeLa lysates | 19864416
TRPML1 |  | Cdc42 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | ERIGC3 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | HSC70 | Fusion protein-pull down assay | Human | 94-298 | In vitro translation | Human | Full-length | In vitro translation | 19117012
TRPML1 |  | HSP40/DNAJC14 | Fusion protein-pull down assay | Human | 94-298 | In vitro translation | Human | Full-length | In vitro translation | 19117012
TRPML1 |  | LAPTM4a | Yeast two-hybrid | Human | Full-length | Yeast | Human | 1-651 | Yeast | 21224396
TRPML1 |  | LAPTM4b | Yeast two-hybrid | Human | Full-length | Yeast | Human | 79-141 | Yeast | 21224396
TRPML1 |  | LAPTM5 | Yeast two-hybrid | Human | Full-length | Yeast | Human | Not specified | Yeast | 21224396
TRPML1 |  | NDKA | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | NovelProtein/NP9 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | PEA-15 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | Peroxisomal membrane protein 2 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | PEX16 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | Rac1 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | Rac2 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | SEC31A | Fusion protein-pull down assay | Human | 1-66 | E. coli | Human | Full-length | HeLa lysates | 19864416
TRPML1 |  | Unnamed protein (BAE) | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601
TRPML1 |  | YIF1B | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPML1 |  | ALG-2/PDCD6 | Co-immunofluorescence staining | ARPE-19 |  |  |  |  |  | 19864416
TRPML1 |  | Cdc42 | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | Cdc42 | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | ERIGC3 | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | HOP/STI1 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML1 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Human | Full-length | 19117012
TRPML1 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML1 |  | HSP40/DNAJC14 | Co-immunofluorescence staining |  | CHO | Human | Full-length | Human | Full-length | 19117012
TRPML1 |  | HSP40/DNAJC14 | Co-immunoprecipitation |  | CHO | Human | Full-length | Human | Full-length | 19117012
TRPML1 |  | HSP40/DNAJC14 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML1 |  | HSP90 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML1 |  | LAPTM4a | Co-immunofluorescence staining |  | ARPE-19 | Human | Full-length | Human | Full-length | 21224396
TRPML1 |  | LAPTM4a | Co-immunoprecipitation |  | HeLa | Human | Full-length | Human | Full-length | 21224396
TRPML1 |  | LAPTM4b | Co-immunofluorescence staining |  | ARPE-19 | Human | Full-length | Human | Full-length | 21224396
TRPML1 |  | LAPTM4b | Co-immunoprecipitation |  | HeLa | Human | Full-length | Human | Full-length | 21224396
TRPML1 |  | LAPTM5 | Co-immunofluorescence staining |  | ARPE-19 | Human | Full-length | Human | Full-length | 21224396
TRPML1 |  | LAPTM5 | Co-immunoprecipitation |  | HeLa | Human | Full-length | Human | Full-length | 21224396
TRPML1 |  | NovelProtein/NP9 | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | NovelProtein/NP9 | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | PEA-15 | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | PEX16 | Split-Ubiquitin Yeast two-hybrid |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | PIP5K1A | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | Rac1 | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | Rac2 | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | RhoG | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | STOML1 | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | STOML1 | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | STOML1 | Co-immunoprecipitation |  | RAW264.7 macrophage | Mouse | Full-length | Not used |  | 23418601
TRPML1 |  | TMEM163 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 25130899
TRPML1 |  | TMEM163 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Mouse | Full-length | 25130899
TRPML1 |  | TPC1 | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC1 | Co-immunofluorescence staining |  | HeLa | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC1 | Co-immunofluorescence staining |  | SK-BR-3 | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC1 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC2 | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC2 | Co-immunofluorescence staining |  | HeLa | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC2 | Co-immunofluorescence staining |  | SK-BR-3 | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TPC2 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML1 |  | TRPML2 | Co-immunofluorescence staining | HEK293 |  |  |  |  |  | 19557826
TRPML1 |  | TRPML2 | Co-immunofluorescence staining |  | CHO | Human | Full-length | Human | Full-length | 19885840
TRPML1 |  | TRPML2 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 19557826
TRPML1 |  | TRPML2 | Co-immunoprecipitation |  | CHO | Human | Full-length | Human | Full-length | 19885840
TRPML1 |  | TRPML2 | Co-immunoprecipitation |  | HEK293 | Human | Full-length (mutation in the pore region) | Human | Full-length (mutation in the pore region) | 20736310
TRPML1 |  | TRPML2 | Fluorescence resonance energy transfer |  | HEK293 | Human | Full-length | Human | Full-length | 16606612
TRPML1 |  | TRPML3 | Co-immunofluorescence staining | HEK293 |  |  |  |  |  | 19557826
TRPML1 |  | TRPML3 | Co-immunofluorescence staining |  | CHO | Human | Full-length | Human | Full-length | 19885840
TRPML1 |  | TRPML3 | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Human | Full-length | 19522758
TRPML1 |  | TRPML3 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 19557826
TRPML1 |  | TRPML3 | Co-immunoprecipitation |  | CHO | Human | Full-length | Human | Full-length | 19885840
TRPML1 |  | TRPML3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length (mutation in the pore region) | Human | Full-length (mutation in the pore region) | 20736310
TRPML1 |  | TRPML3 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151
TRPML1 |  | TRPML3 | Fluorescence resonance energy transfer |  | HEK293 | Human | Full-length | Mouse | Full-length | 16606612
TRPML1 |  | Unnamed protein (BAE) | Co-immunofluorescence staining |  | RAW264.7 macrophage | Mouse | Full-length | Mouse | Full-length | 23418601
TRPML1 |  | YIF1B | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPML1 |  | ALG-2/PDCD6 | Fusion protein-pull down assay | Human | 37-49 | Human | Not determined |  |  | 19864416
TRPML1 |  | SEC31A | Fusion protein-pull down assay | Human | 37-49 | Human | Not determined |  |  | 19864416

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPML1 |  | PKA | In vitro PTM assay | Phosphorylation (Ser-557) |  |  | 17988215
TRPML1 |  | PKA | In vivo PTM assay | Phosphorylation (Ser-559) |  |  | 17988215
TRPML1 |  | PKA | Patch clamp |  |  | Inhibition | 17988215
TRPML1 |  | TRPML2 | Patch clamp |  |  | New channel creation | 19885840
TRPML1 |  | TRPML3 | Patch clamp |  |  | New channel creation | 19885840

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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