# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Rac1 / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (49)
- Disease (0)

Name: Ras-related C3 botulinum toxin substrate 1 (Rac1)
Official Symbol: RAC1 provided by HGNC
Function:
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 5879 | P63000 | DIP-29260N | P63000 | MINT-4999291 | 111817 | P63000 |  | hsa:5879 | 602048
Mouse | 19353 | P63001 | DIP-31545N | P63001 | MINT-1602774 | 202556 | P63001 |  | mmu:19353 |
Rat | 363875 | Q6RUV5 | DIP-37114N | Q6RUV5 | MINT-4577150 | 264467 | Q6RUV5 |  | rno:363875 |

PPI pairs:

TRPM2 | TRPML1

Biological Process:

- GO:0030041 : actin filament polymerization
- GO:0048532 : anatomical structure arrangement
- GO:0097190 : apoptotic signaling pathway
- GO:0002093 : auditory receptor cell morphogenesis
- GO:0007411 : axon guidance
- GO:0045453 : bone resorption
- GO:0048870 : cell motility
- GO:0008283 : cell proliferation
- GO:0045216 : cell-cell junction organization
- GO:0007160 : cell-matrix adhesion
- GO:0021799 : cerebral cortex radially oriented cell migration
- GO:0090103 : cochlea morphogenesis
- GO:0048813 : dendrite morphogenesis
- GO:0071542 : dopaminergic neuron differentiation
- GO:0021831 : embryonic olfactory bulb interneuron precursor migration
- GO:0043652 : engulfment of apoptotic cell
- GO:0003382 : epithelial cell morphogenesis
- GO:0038095 : Fc-epsilon receptor signaling pathway
- GO:0038096 : Fc-gamma receptor signaling pathway involved in phagocytosis
- GO:0006972 : hyperosmotic response
- GO:0006954 : inflammatory response
- GO:0045087 : innate immune response
- GO:0035556 : intracellular signal transduction
- GO:0030032 : lamellipodium assembly
- GO:0051668 : localization within membrane
- GO:0002551 : mast cell chemotaxis
- GO:0032707 : negative regulation of interleukin-23 production
- GO:0048261 : negative regulation of receptor-mediated endocytosis
- GO:0048011 : neurotrophin TRK receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0030838 : positive regulation of actin filament polymerization
- GO:0043065 : positive regulation of apoptotic process
- GO:0045740 : positive regulation of DNA replication
- GO:0010592 : positive regulation of lamellipodium assembly
- GO:0043552 : positive regulation of phosphatidylinositol 3-kinase activity
- GO:0001934 : positive regulation of protein phosphorylation
- GO:0035025 : positive regulation of Rho protein signal transduction
- GO:0030334 : regulation of cell migration
- GO:0050690 : regulation of defense response to virus by virus
- GO:0010310 : regulation of hydrogen peroxide metabolic process
- GO:0060263 : regulation of respiratory burst
- GO:0097178 : ruffle assembly
- GO:0031529 : ruffle organization
- GO:0071526 : semaphorin-plexin signaling pathway
- GO:0007264 : small GTPase mediated signal transduction
- GO:0034446 : substrate adhesion-dependent cell spreading
- GO:0031295 : T cell costimulation
- GO:0016032 : viral process
- GO:0060071 : Wnt signaling pathway, planar cell polarity pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM2 |  | Rac1 | Inference |  |  |  |  |  | Prediction | 25295536
TRPML1 |  | Rac1 | Speculation |  |  |  |  |  | Prediction | 23418601

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPML1 |  | Rac1 | Split-Ubiquitin Yeast two-hybrid | Mouse | Full-length | Yeast | Mouse | Full-length | Yeast | 23418601

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM2 |  | Rac1 | Co-immunofluorescence staining |  | Mouse kidney section | Human | Full-length | Human | Full-length | 25295536
TRPM2 |  | Rac1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 25295536
TRPML1 |  | Rac1 | Co-immunoprecipitation |  | HeLa | Mouse | Full-length | Mouse | Full-length | 23418601

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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