# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPM2 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (6)
- Biological Process (2)
- Disease (0)

Name: Transient receptor potential cation channel subfamily M member 2 (TRPM2)
Official Symbol: TRPM2 provided by HGNC
Function:
Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 7226 | O94759 |  |  |  | 113077 | O94759 | 494 | hsa:7226 | 603749
Mouse | 28240 | Q91YD4 |  |  |  | 205838 | Q91YD4 |  | mmu:28240 |
Rat | 294329 | Q5G856 |  |  |  | 254675 | Q5G856 |  | rno:294329 |

PPI pairs:

Calmodulin | EFHC1 | PKCơ | PTPL1 | Rac1 | UBC

Biological Process:

- GO:0033194 : response to hydroperoxide
- GO:0035725 : sodium ion transmembrane transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM2 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 16461353
TRPM2 |  | EFHC1 | Inference |  |  |  |  |  | Prediction | 22226147
TRPM2 |  | PKCơ | Inference |  |  |  |  |  | Prediction | 24337049
TRPM2 |  | PTPL1 | Inference |  |  |  |  |  | Prediction | 17251321
TRPM2 |  | Rac1 | Inference |  |  |  |  |  | Prediction | 25295536
TRPM2 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | HEK293T | Cell lystaes |  | 21890473
TRPM2 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | ?HCT116 | Cell lystaes |  | 21906983

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM2 |  | Calmodulin | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Not specified | Full-length | 16461353
TRPM2 |  | EFHC1 | Co-immunofluorescence staining |  | HEK293 | Human | Full-length | Mouse | 319-1376 | 22226147
TRPM2 |  | EFHC1 | Co-immunoprecipitation | Mouse brain microsome extracts |  |  |  |  |  | 22226147
TRPM2 |  | EFHC1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Mouse | 319-1376 | 22226147
TRPM2 |  | PKCơ | Co-immunoprecipitation |  | Human pulmonary arterial endothelial cell | Human | 1-846 | Not used |  | 24337049
TRPM2 |  | PTPL1 | Co-immunoprecipitation | Human monocytic U937-ecoR cell |  |  |  |  |  | 17251321
TRPM2 |  | PTPL1 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 17251321
TRPM2 |  | Rac1 | Co-immunofluorescence staining |  | Mouse kidney section | Human | Full-length | Human | Full-length | 25295536
TRPM2 |  | Rac1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 25295536

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM2 |  | Calmodulin | Co-immunoprecipitation | Human | 1-730 | Not specified | Not determined |  |  | 16461353
TRPM2 |  | Calmodulin | Co-immunoprecipitation | Human | 406-416 | Not specified | Not determined |  |  | 16461353
TRPM2 |  | Calmodulin | Co-immunoprecipitation | Human | 1060-1503 | Not specified | Not determined |  |  | 16461353
TRPM2 |  | EFHC1 | Co-immunoprecipitation | Human | 1-2214 | Mouse | Not determined |  |  | 22226147
TRPM2 |  | EFHC1 | Co-immunoprecipitation | Human | 3169-4509 | Mouse | Not determined |  |  | 22226147
TRPM2 |  | PTPL1 | Fusion protein-pull down assay | Human | Not determined | Human | 1099-1199 |  |  | 17251321
TRPM2 |  | PTPL1 | Fusion protein-pull down assay | Human | Not determined | Human | 1888-1990 |  |  | 17251321

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM2 |  | Calmodulin | Calcium measurement |  |  | Activation | 16461353
TRPM2 |  | EFHC1 | Patch clamp |  |  | Activation | 22226147
TRPM2 |  | PKCơ | Calcium measurement |  |  | Activation | 24337049
TRPM2 |  | PKCơ | In vivo PTM assay | Phosphorylation (Ser-29) |  |  | 24337049
TRPM2 |  | PTPL1 | Calcium measurement |  |  | Inhibition | 17251321
TRPM2 |  | PTPL1 | In vivo PTM assay | Dephosphorylation |  |  | 17251321

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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