# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# NHERF1 / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (24)
- Disease (1)

Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF1)
Official Symbol: SLC9A3R1 provided by HGNC
Function:
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 9368 | O14745 | DIP-29092N | O14745 | MINT-4998796 | 114769 | O14745 |  | hsa:9368 | 604990
Mouse | 26941 | P70441 | DIP-38078N | P70441 | MINT-144615 | 205077 | P70441 |  | mmu:26941 |
Rat | 59114 | Q9JJ19 | DIP-35760N | Q9JJ19 | MINT-4568572 | 859506 | Q9JJ19 |  | rno:59114 |

PPI pairs:

TRPC4 | TRPC5

Biological Process:

- GO:0030036 : actin cytoskeleton organization
- GO:0007191 : adenylate cyclase-activating dopamine receptor signaling pathway
- GO:0006915 : apoptotic process
- GO:0032782 : bile acid secretion
- GO:0030643 : cellular phosphate ion homeostasis
- GO:0034613 : cellular protein localization
- GO:0034635 : glutathione transport
- GO:0030033 : microvillus assembly
- GO:0030336 : negative regulation of cell migration
- GO:0008285 : negative regulation of cell proliferation
- GO:0070373 : negative regulation of ERK1 and ERK2 cascade
- GO:0014067 : negative regulation of phosphatidylinositol 3-kinase cascade
- GO:0010642 : negative regulation of platelet-derived growth factor receptor signaling pathway
- GO:0051898 : negative regulation of protein kinase B signaling cascade
- GO:0010766 : negative regulation of sodium ion transport
- GO:0032416 : negative regulation of sodium
- GO:0060158 : phospholipase C-activating dopamine receptor signaling pathway
- GO:0006461 : protein complex assembly
- GO:0044062 : regulation of excretion
- GO:0045859 : regulation of protein kinase activity
- GO:0032415 : regulation of sodium
- GO:0097291 : renal phosphate ion absorption
- GO:0003096 : renal sodium ion transport
- GO:0016055 : Wnt receptor signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:604990 : Nephrolithiasis/osteoporosis, hypophosphatemic, 2 (OMIM:612287 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC4 |  | NHERF1 | Inference |  |  |  |  |  | Prediction | 10980202
TRPC4 |  | NHERF1 | Yeast two-hybrid | Human | 686-977 | Human | Brain | cDNA library |  | 18048348
TRPC5 |  | NHERF1 | Inference |  |  |  |  |  | Prediction | 10980202

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 1-102 | E. coli | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 144-247 | E. coli | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | 1-107 | E. coli | 10980202
TRPC5 |  | NHERF1 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | 1-107 | E. coli | 10980202

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | NHERF1 | Co-immunofluorescence staining |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 18048348
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 12154080
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | NHERF1 | Co-immunoprecipitation | Human fetal brain astrocytes |  |  |  |  |  | 15540229
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 10980202
TRPC4 |  | NHERF1 | Co-immunoprecipitation | Mouse brain tissue |  |  |  |  |  | 10980202

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | NHERF1 | Co-immunoprecipitation | Human | 975-977 (in ơ varint) | Human | 130-328 |  |  | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | 975-977 (in ơ varint) | Human | 1-102 |  |  | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | 975-977 (in ơ varint) | Human | 144-247 |  |  | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Mouse | 688-974 | Mouse | 1-107 |  |  | 10980202
TRPC5 |  | NHERF1 | Fusion protein-pull down assay | Mouse | 648-975 | Mouse | 1-107 |  |  | 10980202

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC4 |  | NHERF1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 12154080

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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