# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# STIM1 / TRP channels

- Basic Information
- External Database
- PPI pairs (7)
- Biological Process (4)
- Disease (1)

Name: Stromal interaction molecule 1 (STIM1)
Official Symbol: STIM1 provided by HGNC
Function:
Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 6786 | Q13586 | DIP-31121N | Q13586 | UNK-5391628 | 112662 | Q13586 |  | hsa:6786 | 605921
Mouse | 20866 | P70302 | DIP-48770N |  | MINT-4135875 | 203538 | P70302 |  | mmu:20866 |
Rat | 361618 | P84903 |  |  | UNK-5383801 | 262820 | P84903 |  | rno:361618 |

PPI pairs:

TRPA1 | TRPC1 | TRPC2 | TRPC3 | TRPC4 | TRPC5 | TRPC6

Biological Process:

- GO:0032237 : activation of store-operated calcium channel activity
- GO:0007596 : blood coagulation
- GO:0006816 : calcium ion transport
- GO:0005513 : detection of calcium ion

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:605921 : Immune dysfunction, with T-cell inactivation due to calcium entry defect 2 (OMIM:612783 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPA1 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 23994313
TRPC1 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 16906149
TRPC2 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 16906149
TRPC3 |  | STIM1 | Speculation |  |  |  |  |  | Prediction | 22640869
TRPC4 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 16906149
TRPC5 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 16906149
TRPC6 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 19260825

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC1 |  | STIM1 | Fusion protein-pull down assay | Not specified | 1-347 | E. coli | Not used |  | HSG cell lysates | 17224452

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPA1 |  | STIM1 | Co-immunoprecipitation | MEG-01 |  |  |  |  |  | 23994313
TRPC1 |  | STIM1 | Co-immunofluorescence staining |  | HEK293 | Human | Full-length | Human | Full-length | 16906149
TRPC1 |  | STIM1 | Co-immunofluorescence staining | Mouse pulmonary arterial smooth muscle cell |  |  |  |  |  | 23034388
TRPC1 |  | STIM1 | Co-immunofluorescence staining |  | HSG cell | Human | Full-length | Not specified | Full-length | 17224452
TRPC1 |  | STIM1 | Co-immunofluorescence staining | HSG cell |  |  |  |  |  | 19897728
TRPC1 |  | STIM1 | Co-immunofluorescence staining | Human saphenous vein vascular smooth muscle cell |  |  |  |  |  | 18802022
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 19228695
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 16870612
TRPC1 |  | STIM1 | Co-immunoprecipitation | Mouse pulmonary arterial smooth muscle cell |  |  |  |  |  | 19332490
TRPC1 |  | STIM1 | Co-immunoprecipitation | Rat brain tissue |  |  |  |  |  | 16906149
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 16906149
TRPC1 |  | STIM1 | Co-immunoprecipitation | Mouse pulmonary arterial smooth muscle cell |  |  |  |  |  | 23034388
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18249094
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Human | Full-length | 24464579
TRPC1 |  | STIM1 | Co-immunoprecipitation | Mouse bone marrow derived macrophages |  |  |  |  |  | 25013939
TRPC1 |  | STIM1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 18326500
TRPC1 |  | STIM1 | Co-immunoprecipitation | HSG cell |  |  |  |  |  | 18430726
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Not used |  | Not specified | Full-length | 17224452
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | A7r5 | Not used |  | Not specified | Full-length | 17224452
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18644792
TRPC1 |  | STIM1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 23395841
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human atrial myocyte |  |  |  |  |  | 23686296
TRPC1 |  | STIM1 | Co-immunoprecipitation | HSG cell |  |  |  |  |  | 19897728
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | HEK293T | Rat | Full-length | Rat | Full-length | 22201561
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human saphenous vein vascular smooth muscle cell |  |  |  |  |  | 18802022
TRPC1 |  | STIM1 | Co-immunoprecipitation |  | Human saphenous vein vascular smooth muscle cell | Human | Full-length | Not specified | Full-length | 18802022
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 17892531
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human parathyroid tissue |  |  |  |  |  | 20194530
TRPC1 |  | STIM1 | Co-immunoprecipitation | SH-SY5Y |  |  |  |  |  | 22446186
TRPC1 |  | STIM1 | Co-immunoprecipitation | HSG cell |  |  |  |  |  | 20488539
TRPC1 |  | STIM1 | Co-immunoprecipitation | Rat normal small intestine cell line IEC-6 |  |  |  |  |  | 22592407
TRPC1 |  | STIM1 | Co-immunoprecipitation | Rat normal small intestine cell line IEC-6 (IEC-Cdx2L1 stable cell) |  |  |  |  |  | 22592407
TRPC1 |  | STIM1 | Co-immunoprecipitation | Caco-2 cell |  |  |  |  |  | 22592407
TRPC2 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 16906149
TRPC3 |  | STIM1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 22640869
TRPC3 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 23068106
TRPC3 |  | STIM1 | Co-immunoprecipitation |  |  | Not specified | Full-length (mutant) | Human | Full-length | 24464579
TRPC4 |  | STIM1 | Co-immunoprecipitation | Human glomerular mesangial cell |  |  |  |  |  | 19307462
TRPC4 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 16906149
TRPC4 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Human | Full-length | 24464579
TRPC4 |  | STIM1 | Co-immunoprecipitation |  | Mouse lung endothelial cell | Mouse | Full-length | Human | Full-length | 22210847
TRPC5 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 16906149
TRPC5 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Human | Full-length | 24464579
TRPC5 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 17486119
TRPC5 |  | STIM1 | Co-immunoprecipitation |  | HEK293T | Rat | Full-length | Rat | Full-length | 22201561
TRPC6 |  | STIM1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 19260825
TRPC6 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 23068106
TRPC6 |  | STIM1 | Co-immunoprecipitation |  |  | Not specified | Full-length (mutant) | Human | Full-length | 24464579

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | STIM1 | Co-immunoprecipitation | Human | Not determined | Human | 251-535 |  |  | 16906149
TRPC1 |  | STIM1 | Co-immunoprecipitation | Rat | Not determined | Rat | 1798-1887 |  |  | 22201561
TRPC1 |  | STIM1 | Fusion protein-pull down assay | Human | Not determined | Human | 251-535 |  |  | 16906149
TRPC1 |  | STIM1 | Fusion protein-pull down assay | Human | 1-347 | Human | Not determined |  |  | 17224452
TRPC2 |  | STIM1 | Co-immunoprecipitation | Mouse | Not determined | Human | 251-535 |  |  | 16906149
TRPC4 |  | STIM1 | Co-immunoprecipitation | Mouse | Not determined | Human | 251-535 |  |  | 16906149
TRPC5 |  | STIM1 | Co-immunoprecipitation | Rat | Not determined | Rat | 1798-1887 |  |  | 22201561

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC1 |  | STIM1 | Patch clamp |  |  | Activation | 16906149

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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