# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPV2 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (9)
- Biological Process (5)
- Disease (0)

Name: Transient receptor potential cation channel subfamily V member 2 (TRPV2)
Official Symbol: TRPV2 provided by HGNC
Function:
Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 51393 | Q9Y5S1 |  |  |  | 119520 | Q9Y5S1 | 508 | hsa:51393 | 606676
Mouse | 22368 | Q9WTR1 |  |  |  | 204537 | Q9WTR1 |  | mmu:22368 |
Rat | 29465 | Q9WUD2 |  |  |  | 248108 | Q9WUD2 |  | rno:29465 |

PPI pairs:

ACBD3 | AKAP5/AKAP150 | Calmodulin | PKA | PKARIIơ | RGA/RAG1AP1 | SYNE1 | TRPV1
UBC

Biological Process:

- GO:0045773 : positive regulation of axon extension
- GO:0090280 : positive regulation of calcium ion import
- GO:0009408 : response to heat
- GO:0009266 : response to temperature stimulus
- GO:0007600 : sensory perception

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV2 |  | ACBD3 | Inference |  |  |  |  |  | Prediction | 15249591
TRPV2 |  | AKAP5/AKAP150 | Speculation |  |  |  |  |  | Prediction | 18701070
TRPV2 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 20686800
TRPV2 |  | PKA | Inference |  |  |  |  |  | Prediction | 15249591
TRPV2 |  | PKARIIơ | Inference |  |  |  |  |  | Prediction | 15249591
TRPV2 |  | RGA/RAG1AP1 | Yeast two-hybrid | Rat | N-terminus | Rat | Brain | cDNA library |  | 14991772
TRPV2 |  | SYNE1 | Yeast two-hybrid | Mouse | 1-167 | Human | Heart | cDNA library |  | 16875688
TRPV2 |  | TRPV1 | Affinity purification-mass spectrometry | Not used |  | Mouse | Dorsal root ganglion tissue | Lysates |  | 24036102
TRPV2 |  | TRPV1 | Inference |  |  |  |  |  | Prediction | 15713749
TRPV2 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | HEK293T | Cell lystaes |  | 21890473
TRPV2 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | ?HCT116 | Cell lystaes |  | 21906983

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV2 |  | Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined | Not specified | E. coli | 20926660
TRPV2 |  | SYNE1 | Fusion protein-pull down assay | Not used |  | Rat heart lysates | Human | 1-476 (in GSRP-56 Variant) | E. coli | 16875688

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV2 |  | ACBD3 | Co-immunoprecipitation | RBL-2H3 |  |  |  |  |  | 15249591
TRPV2 |  | AKAP5/AKAP150 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 18701070
TRPV2 |  | PKARIIơ | Co-immunoprecipitation | RBL-2H3 |  |  |  |  |  | 15249591
TRPV2 |  | RGA/RAG1AP1 | Co-immunofluorescence staining |  | HEK293 | Rat | Full-length | Mouse | Full-length | 14991772
TRPV2 |  | RGA/RAG1AP1 | Co-immunoprecipitation |  | RBL-2H3 | Not used |  | Mouse | Full-length | 14991772
TRPV2 |  | RGA/RAG1AP1 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Mouse | Full-length | 14991772
TRPV2 |  | RGA/RAG1AP1 | Co-immunoprecipitation | RBL-2H3 |  |  |  |  |  | 15547947
TRPV2 |  | RGA/RAG1AP1 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Human | Full-length | 15547947
TRPV2 |  | TRPV1 | Co-immunofluorescence staining | Rat dorsal root ganglion tissue |  |  |  |  |  | 16115206
TRPV2 |  | TRPV1 | Co-immunofluorescence staining |  | HEK293 | Rat | Full-length | Rat | Full-length | 16237318
TRPV2 |  | TRPV1 | Co-immunofluorescence staining | Rat dorsal root ganglion section |  |  |  |  |  | 16237318
TRPV2 |  | TRPV1 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Rat | Full-length | 16115206
TRPV2 |  | TRPV1 | Co-immunoprecipitation | Rat brain cortical lysates |  |  |  |  |  | 16115206
TRPV2 |  | TRPV1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 16237318
TRPV2 |  | TRPV1 | Co-immunoprecipitation |  | Rat dorsal root ganglion derived cell line F11 | Rat | Full-length | Rat | Full-length | 16237318
TRPV2 |  | TRPV1 | Co-immunoprecipitation | Rat dorsal root ganglion lysates |  |  |  |  |  | 16237318
TRPV2 |  | TRPV1 | Co-immunoprecipitation | Rat spinal cord lysates |  |  |  |  |  | 16237318
TRPV2 |  | TRPV1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Rat | Full-length | 15713749

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV2 |  | Calmodulin | Fluorescence probe labeling | Human | 654-683 | Not specified | Not determined |  | 191 nM | 20686800
TRPV2 |  | Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined |  |  | 20926660
TRPV2 |  | SYNE1 | Fusion protein-pull down assay | Rat | Not determined | Human | 1-258 (in GSRP-56 Variant) |  |  | 16875688
TRPV2 |  | SYNE1 | Fusion protein-pull down assay | Rat | Not determined | Human | 1-258 |  |  | 16875688

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV2 |  | PKA | In vitro PTM assay | Phosphorylation |  |  | 15249591
TRPV2 |  | RGA/RAG1AP1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 15547947

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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