# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# IP3R2 / TRP channels

- Basic Information
- External Database
- PPI pairs (5)
- Biological Process (10)
- Disease (0)

Name: Inositol 1,4,5-trisphosphate receptor type 2 (IP3R2)
Official Symbol: ITPR2 provided by HGNC
Function:
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 3709 | Q14571 | DIP-42028N | Q14571 | MINT-157881 | 109914 | Q14571 |  | hsa:3709 | 600144
Mouse | 16439 | Q9Z329 |  |  | UNK-5562109 | 200848 | Q9Z329 |  | mmu:16439 |
Rat | 81678 | P29995 |  |  | UNK-6030828 | 249575 | P29995 |  | rno:81678 |

PPI pairs:

TRPC1 | TRPC2 | TRPC3 | TRPC4 | TRPC6

Biological Process:

- GO:0007202 : activation of phospholipase C activity
- GO:0071320 : cellular response to cAMP
- GO:0006112 : energy reserve metabolic process
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0050796 : regulation of insulin secretion
- GO:0001666 : response to hypoxia
- GO:0044281 : small molecule metabolic process

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 10970773
TRPC2 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 15329338
TRPC3 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC4 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 11163362
TRPC6 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 18191041

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC4 |  | IP3R2 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Rat | 2544-2701 | E. coli | 11163362
TRPC4 |  | IP3R2 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2544-2701 | Yeast | 11163362

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 11336651
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18068335
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 16870612
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18191041
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Rat astrocyte |  |  |  |  |  | 16905188
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18249094
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 15121806
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 12196544
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 23228564
TRPC1 |  | IP3R2 | Co-immunoprecipitation | MEG-01 |  |  |  |  |  | 23228564
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 10970773
TRPC1 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 17681754
TRPC2 |  | IP3R2 | Co-immunofluorescence staining | Mouse splenic erythroblast |  |  |  |  |  | 15329338
TRPC2 |  | IP3R2 | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Rat | Full-length | 15329338
TRPC2 |  | IP3R2 | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Rat | Full-length | 22088247
TRPC3 |  | IP3R2 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 11524429
TRPC3 |  | IP3R2 | Co-immunoprecipitation | Sheep uterine arterial endothelial cell |  |  |  |  |  | 16899571
TRPC6 |  | IP3R2 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18191041

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC2 |  | IP3R2 | Co-immunoprecipitation | Mouse | 1001-1036 | Rat | Not determined |  |  | 15329338
TRPC3 |  | IP3R2 | Fusion protein-pull down assay | Mouse | 725-848 | Not specified | 675-695 |  |  | 11290752
TRPC4 |  | IP3R2 | Fusion protein-pull down assay | Human | 785-868 | Rat | 2556-2713 |  |  | 11163362

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC1 |  | IP3R2 | Calcium measurement |  |  | Activation | 12196544
TRPC1 |  | IP3R2 | Patch clamp |  |  | Activation | 12196544

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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