# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Bkca / TRP channels

- Basic Information
- External Database
- PPI pairs (5)
- Biological Process (11)
- Disease (1)

Name: Calcium-activated potassium channel subunit alpha-1 (Bkca)
Official Symbol: KCNMA1 provided by HGNC
Function:
Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 3778 | Q12791 | DIP-29729N | Q12791 | MINT-4825316 | 109979 | Q12791 |  | hsa:3778 | 600150
Mouse | 16531 | Q08460 | DIP-42413N | Q08460 | MINT-1203219 | 200913 | Q08460 |  | mmu:16531 |
Rat | 83731 | Q62976 | DIP-40510N | Q62976 | MINT-1203282 | 249823 | Q62976 |  | rno:83731 |

PPI pairs:

TRPC1 | TRPC3 | TRPC6 | TRPV1 | TRPV4

Biological Process:

- GO:0007596 : blood coagulation
- GO:0030007 : cellular potassium ion homeostasis
- GO:0045794 : negative regulation of cell volume
- GO:0043065 : positive regulation of apoptotic process
- GO:0042391 : regulation of membrane potential
- GO:0051592 : response to calcium ion
- GO:0034465 : response to carbon monoxide
- GO:0001666 : response to hypoxia
- GO:0006970 : response to osmotic stress
- GO:0060083 : smooth muscle contraction involved in micturition
- GO:0007268 : synaptic transmission

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:600150 : Generalized epilepsy and paroxysmal dyskinesia (OMIM:609446 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | BKca | Inference |  |  |  |  |  | Prediction | 19168436
TRPC3 |  | BKca | Inference |  |  |  |  |  | Prediction | 19052171
TRPC6 |  | BKca | Inference |  |  |  |  |  | Prediction | 19052171
TRPV1 |  | BKca | Inference |  |  |  |  |  | Prediction | 24147119
TRPV4 |  | Bkca | Inference |  |  |  |  |  | Prediction | 25139746

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC3 |  | BKca | Fusion protein-pull down assay | Not used |  | Mouse podocyte lysates | Chicken | Full-length | E. coli | 19052171
TRPC6 |  | BKca | Fusion protein-pull down assay | Not used |  | Mouse podocyte lysates | Chicken | Full-length | E. coli | 19052171

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | BKca | Co-immunofluorescence staining | Rat aortic vascular smooth muscle cell |  |  |  |  |  | 19168436
TRPC1 |  | BKca | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 19168436
TRPC1 |  | BKca | Co-immunoprecipitation | Rat vascular smooth muscle cell |  |  |  |  |  | 19168436
TRPC1 |  | Bkca | Co-immunoprecipitation | Porcine coronary artery |  |  |  |  |  | 25139746
TRPC3 |  | BKca | Co-immunofluorescence staining |  | HEK293T | Not specified | Full-length | Not specified | Full-length | 19052171
TRPC3 |  | BKca | Co-immunoprecipitation | Mouse podocyte lysates |  |  |  |  |  | 19052171
TRPC3 |  | BKca | Co-immunoprecipitation |  | HEK293T | Not specified | Full-length | Not specified | Full-length | 19052171
TRPC6 |  | BKca | Co-immunofluorescence staining |  | HEK293T | Not specified | Full-length | Not specified | Full-length | 19052171
TRPC6 |  | BKca | Co-immunoprecipitation | Mouse podocyte lysates |  |  |  |  |  | 19052171
TRPC6 |  | BKca | Co-immunoprecipitation |  | HEK293T | Not specified | Full-length | Not specified | Full-length | 19052171
TRPV1 |  | BKca | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Human | Full-length | 24147119
TRPV1 |  | BKca | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 24147119
TRPV1 |  | BKca | Co-immunoprecipitation | Rat dorsal root ganglion neuron |  |  |  |  |  | 24147119
TRPV4 |  | Bkca | Co-immunoprecipitation | Porcine coronary artery |  |  |  |  |  | 25139746

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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