# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPC4 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (38)
- Biological Process (4)
- Disease (0)

Name: Short transient receptor potential channel 4 (TRPC4)
Official Symbol: TRPC4 provided by HGNC
Function:
Form a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 7223 | Q9UBN4 | DIP-35695N | Q9UBN4 | MINT-157838 | 113074 | Q9UBN4 | 489 | hsa:7223 | 603651
Mouse | 22066 | Q9QUQ5 | DIP-40848N |  | MINT-144626 | 204330 | Q9QUQ5 |  | mmu:22066 |
Rat | 84494 | O35119 |  |  | MINT-5022099 | 249993 | O35119 |  | rno:84494 |

PPI pairs:

80K-H | Calmodulin | Cav1.2 | Caveolin-1 | D2R | FKBP5 | FKBP52/FKBP4 | FYN
GJA1 | Gơi2 | Homer-3 | IP3R1 | IP3R2 | IP3R3 | LYN | MxA
NHERF1 | NHERF2 | PLCƣ1 | Protein 4.1 | RNF24 | SESTD1 | SHANK | STIM1
Src | TARBP2 | TRPC1 | TRPC3 | TRPC5 | TRPC6 | TRPP1 | VASP
VE-cadherin | ZO-1 | ơ1-syntrophin | ơII-spectrin | Ƣ-catenin | ƢV-spectrin

Biological Process:

- GO:0007411 : axon guidance
- GO:0070509 : calcium ion import
- GO:0014051 : gamma-aminobutyric acid secretion
- GO:0051924 : regulation of calcium ion transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC4 |  | 80K-H | Speculation |  |  |  |  |  | Prediction | 19801576
TRPC4 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC4 |  | Cav1.2 | Inference |  |  |  |  |  | Prediction | 21672930
TRPC4 |  | Caveolin-1 | Inference |  |  |  |  |  | Prediction | 17416589
TRPC4 |  | D2R | Inference |  |  |  |  |  | Prediction | 18261457
TRPC4 |  | FKBP5 | Inference |  |  |  |  |  | Prediction | 23375350
TRPC4 |  | FKBP52/FKBP4 | Inference |  |  |  |  |  | Prediction | 15199065
TRPC4 |  | FYN | Inference |  |  |  |  |  | Prediction | 16144838
TRPC4 |  | GJA1 | Inference |  |  |  |  |  | Prediction | 15540229
TRPC4 |  | Gơi2 | Inference |  |  |  |  |  | Prediction | 22457348
TRPC4 |  | Ƣ-catenin | Inference |  |  |  |  |  | Prediction | 19996314
TRPC4 |  | ƢV-spectrin | Yeast two-hybrid | Human | 686-977 | Human | Brain | cDNA library |  | 18048348
TRPC4 |  | Homer-3 | Inference |  |  |  |  |  | Prediction | 14505576
TRPC4 |  | IP3R1 | Inference |  |  |  |  |  | Prediction | 11163362
TRPC4 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 11163362
TRPC4 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC4 |  | LYN | Inference |  |  |  |  |  | Prediction | 16144838
TRPC4 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897
TRPC4 |  | NHERF1 | Inference |  |  |  |  |  | Prediction | 10980202
TRPC4 |  | NHERF1 | Yeast two-hybrid | Human | 686-977 | Human | Brain | cDNA library |  | 18048348
TRPC4 |  | NHERF2 | Inference |  |  |  |  |  | Prediction | 15590898
TRPC4 |  | ơ1-syntrophin | Inference |  |  |  |  |  | Prediction | 19812031
TRPC4 |  | ơII-spectrin | Yeast two-hybrid | Human | 686-977 | Human | Brain | cDNA library |  | 18048348
TRPC4 |  | PLCƣ1 | Inference |  |  |  |  |  | Prediction | 12437926
TRPC4 |  | PLCƢ1 | Inference |  |  |  |  |  | Prediction | 10980202
TRPC4 |  | Protein 4.1 | Inference |  |  |  |  |  | Prediction | 16254212
TRPC4 |  | RNF24 | Inference |  |  |  |  |  | Prediction | 17850865
TRPC4 |  | SESTD1 | Yeast two-hybrid | Mouse | 615-974 | Human | Aortic | cDNA library |  | 20164195
TRPC4 |  | SHANK | Inference |  |  |  |  |  | Prediction | 25049082
TRPC4 |  | SRC | Inference |  |  |  |  |  | Prediction | 16144838
TRPC4 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 16906149
TRPC4 |  | TARBP2 | Yeast two-hybrid | Mouse | Not specified | Mouse | Brain | cDNA library |  | 24563462
TRPC4 |  | TRPC1 | Inference |  |  |  |  |  | Prediction | 11301024
TRPC4 |  | TRPC3 | Inference |  |  |  |  |  | Prediction | 16537542
TRPC4 |  | TRPC5 | Inference |  |  |  |  |  | Prediction | 12032305
TRPC4 |  | TRPC6 | Inference |  |  |  |  |  | Prediction | 12857742
TRPC4 |  | TRPP1 | Inference |  |  |  |  |  | Prediction | 18256307
TRPC4 |  | VASP | Inference |  |  |  |  |  | Prediction | 17913834
TRPC4 |  | VE-cadherin | Inference |  |  |  |  |  | Prediction | 19996314
TRPC4 |  | ZO-1 | Inference |  |  |  |  |  | Prediction | 15540229

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Not used |  | Bovine adrenal cortex lysates | Xenopus | Full-length | In vitro translation | 11311128
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-320 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Not specified | Not specified | Not specified | 11290752
TRPC4 |  | Caveolin-1 | Fusion protein-pull down assay | Not used |  | Mouse lung endothelium cell lysates | Not specified | Full-length | E. coli | 17416589
TRPC4 |  | D2R | Fusion protein-pull down assay | Human | 621-893 | E. coli | Human | 131-151 | E. coli | 18261457
TRPC4 |  | FKBP52/FKBP4 | Co-immunoprecipitation | Bovine | Full-length | Sf9 lysates | Human | Full-length | Sf9 lysates | 15199065
TRPC4 |  | Gơi2 | Fusion protein-pull down assay | Mouse | Full | E. coli | Human | Full | E. coli | 22457348
TRPC4 |  | ƢV-spectrin | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 3232-3674 | E. coli | 18048348
TRPC4 |  | IP3R1 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2520-2670 | Yeast | 11163362
TRPC4 |  | IP3R2 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Rat | 2544-2701 | E. coli | 11163362
TRPC4 |  | IP3R2 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2544-2701 | Yeast | 11163362
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Human | 669-698 | E. coli | 11290752
TRPC4 |  | IP3R3 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2556-2713 | Yeast | 11163362
TRPC4 |  | MxA | Fusion protein-pull down assay | Human | 3-183 | In vitro translation | Human | 572-662 | E. coli | 15757897
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 1-102 | E. coli | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 144-247 | E. coli | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | 1-107 | E. coli | 10980202
TRPC4 |  | ơ1-syntrophin | Fusion protein-pull down assay | Not used |  | C57BL/10 mouse muscle cell lysates | Mouse | 75-170 | E. coli | 19812031
TRPC4 |  | ơII-spectrin | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 1998-2472 | E. coli | 18048348
TRPC4 |  | PLCƣ1 | Yeast two-hybrid | Human | 1-330 | Yeast | Rat | 750-1290 | Yeast | 12437926
TRPC4 |  | RNF24 | Fusion protein-pull down assay | Not specified | 3-183 | In vitro translation | Human | 98-148 | E. coli | 17850865
TRPC4 |  | SRC | Fusion protein-pull down assay | Mouse | 626-974 | In vitro translation | Chicken | Full-length | E. coli | 16407161
TRPC4 |  | TARBP2 | Fusion protein-pull down assay | Mouse | 625-974 | E. coli | Mouse | Full-length | HEK293 lysates | 24563462

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | 80K-H | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Human | Full-length | 19801576
TRPC4 |  | Cav1.2 | Co-immunoprecipitation | Chick embryonic heart lysates |  |  |  |  |  | 21672930
TRPC4 |  | Caveolin-1 | Co-immunofluorescence staining | Mouse lung endothelium cell |  |  |  |  |  | 17416589
TRPC4 |  | Caveolin-1 | Co-immunoprecipitation | Mouse lung endothelium cell |  |  |  |  |  | 17416589
TRPC4 |  | FKBP5 | Co-immunoprecipitation | Rat pumonary aortic endothelial cell |  |  |  |  |  | 23375350
TRPC4 |  | FKBP52/FKBP4 | Co-immunoprecipitation | Rat cerebral cortex |  |  |  |  |  | 15199065
TRPC4 |  | FKBP52/FKBP4 | Co-immunoprecipitation | Rat pumonary aortic endothelial cell |  |  |  |  |  | 23375350
TRPC4 |  | FYN | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 18048348
TRPC4 |  | FYN | Co-immunoprecipitation | COS-7 |  |  |  |  |  | 16144838
TRPC4 |  | FYN | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | GJA1 | Co-immunofluorescence staining | Human fetal brain astrocytes |  |  |  |  |  | 15540229
TRPC4 |  | GJA1 | Co-immunoprecipitation | Human fetal brain astrocytes |  |  |  |  |  | 15540229
TRPC4 |  | Gơi2 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 24011658
TRPC4 |  | Gơi2 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 22457348
TRPC4 |  | Gơi2 | Co-immunoprecipitation | Rat brain extract |  |  |  |  |  | 22457348
TRPC4 |  | Ƣ-catenin | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Not specified | Full-length | 19996314
TRPC4 |  | Ƣ-catenin | Co-immunoprecipitation |  | Human microvascular endpthelial cell-1 | Mouse | Full-length | Not specified | Full-length | 19996314
TRPC4 |  | Ƣ-catenin | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Not specified | Full-length | 19996314
TRPC4 |  | Homer-3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC4 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC4 |  | LYN | Co-immunoprecipitation | COS-7 |  |  |  |  |  | 16144838
TRPC4 |  | LYN | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | MxA | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15757897
TRPC4 |  | NHERF1 | Co-immunofluorescence staining |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 18048348
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 12154080
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | NHERF1 | Co-immunoprecipitation | Human fetal brain astrocytes |  |  |  |  |  | 15540229
TRPC4 |  | NHERF1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 10980202
TRPC4 |  | NHERF1 | Co-immunoprecipitation | Mouse brain tissue |  |  |  |  |  | 10980202
TRPC4 |  | NHERF2 | Co-immunofluorescence staining | Rat kidney tissue |  |  |  |  |  | 15590898
TRPC4 |  | NHERF2 | Co-immunoprecipitation | Rat kidney tissue |  |  |  |  |  | 15590898
TRPC4 |  | ơ1-syntrophin | Co-immunoprecipitation | C57BL/10 mouse muscle cell |  |  |  |  |  | 19812031
TRPC4 |  | ơII-spectrin | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 18048348
TRPC4 |  | PLCƢ1 | Co-immunofluorescence staining |  | Neonatal rat ventricular myocytes | Not used |  | Mouse | Full-length | 25049082
TRPC4 |  | PLCƢ1 | Co-immunoprecipitation |  | Neonatal rat ventricular myocytes | Human | Full-length | Mouse | Full-length | 25049082
TRPC4 |  | PLCƢ1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 10980202
TRPC4 |  | PLCƢ1 | Co-immunoprecipitation | Mouse brain tissue |  |  |  |  |  | 10980202
TRPC4 |  | Protein 4.1 | Co-immunoprecipitation | Rat pumonary aortic endothelial cell |  |  |  |  |  | 16254212
TRPC4 |  | Protein 4.1 | Co-immunoprecipitation | Rat pumonary aortic endothelial cell |  |  |  |  |  | 22534489
TRPC4 |  | SESTD1 | Co-immunoprecipitation |  | HEK293-HM1 stable cell line | Mouse | Full-length | Human | Full-length | 20164195
TRPC4 |  | SHANK | Co-immunoprecipitation |  | Neonatal rat ventricular myocytes | Human | Full-length | Not used |  | 25049082
TRPC4 |  | SRC | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not specifed | Full-length | 16144838
TRPC4 |  | STIM1 | Co-immunoprecipitation | Human glomerular mesangial cell |  |  |  |  |  | 19307462
TRPC4 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 16906149
TRPC4 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Human | Full-length | 24464579
TRPC4 |  | STIM1 | Co-immunoprecipitation |  | Mouse lung endothelial cell | Mouse | Full-length | Human | Full-length | 22210847
TRPC4 |  | TARBP2 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 24563462
TRPC4 |  | TRPC1 | Co-immunofluorescence staining | BAE-1 |  |  |  |  |  | 16818374
TRPC4 |  | TRPC1 | Co-immunofluorescence staining | Human glomerular mesangial cell |  |  |  |  |  | 16418302
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 11301024
TRPC4 |  | TRPC1 | Co-immunoprecipitation | BAE-1 |  |  |  |  |  | 16818374
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Human glomerular mesangial cell |  |  |  |  |  | 19307462
TRPC4 |  | TRPC1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 12032305
TRPC4 |  | TRPC1 | Co-immunoprecipitation | C57BL/10 mouse muscle cell |  |  |  |  |  | 19812031
TRPC4 |  | TRPC1 | Co-immunoprecipitation |  | Sf9 | Bovine | Full-length | Human | Full-length | 12377790
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Rat brain synaptome |  |  |  |  |  | 12377790
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 16270640
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Mouse lung endothelium cell |  |  |  |  |  | 17416589
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Human glomerular mesangial cell |  |  |  |  |  | 16418302
TRPC4 |  | TRPC1 | Co-immunoprecipitation |  | Mouse lung endothelial cell | Mouse | Full-length | Mouse | Full-length | 22210847
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 12857742
TRPC4 |  | TRPC1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 17892531
TRPC4 |  | TRPC1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 12032305
TRPC4 |  | TRPC1 | Fluorescence resonance energy transfer | Rat pumonary aortic endothelial cell |  |  |  |  |  | 22534489
TRPC4 |  | TRPC3 | Co-immunofluorescence staining | Porcine primary aortic endothelial cell |  |  |  |  |  | 16537542
TRPC4 |  | TRPC3 | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 12857742
TRPC4 |  | TRPC3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 16537542
TRPC4 |  | TRPC3 | Co-immunoprecipitation | Porcine primary aortic endothelial cell |  |  |  |  |  | 16537542
TRPC4 |  | TRPC3 | Co-immunoprecipitation | TRPC3 dnTRPC4 DTG mouse heart lysataes |  |  |  |  |  | 20351294
TRPC4 |  | TRPC5 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 12032305
TRPC4 |  | TRPC5 | Co-immunoprecipitation |  | Sf9 | Bovine | Full-length | Mouse | Full-length | 12377790
TRPC4 |  | TRPC5 | Co-immunoprecipitation | Rat brain synaptome |  |  |  |  |  | 12377790
TRPC4 |  | TRPC5 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 16270640
TRPC4 |  | TRPC5 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 12032305
TRPC4 |  | TRPC5 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 17624425
TRPC4 |  | TRPC6 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not specifed | Full-length | 17486119
TRPC4 |  | TRPC6 | Co-immunoprecipitation | Rat brain microsome |  |  |  |  |  | 12857742
TRPC4 |  | TRPP1 | Co-immunoprecipitation |  | Human glomerular mesangial cell | Bovine | Full-length | Not used |  | 18256307
TRPC4 |  | VASP | Co-immunofluorescence staining | Human glomerular mesangial cell |  |  |  |  |  | 17913834
TRPC4 |  | VASP | Co-immunoprecipitation | Human glomerular mesangial cell |  |  |  |  |  | 17913834
TRPC4 |  | VE-cadherin | Co-immunoprecipitation |  | Human microvascular endpthelial cell-1 | Mouse | Full-length | Mouse | Full-length | 19996314
TRPC4 |  | VE-cadherin | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 19996314
TRPC4 |  | ZO-1 | Co-immunofluorescence staining | Human fetal brain astrocytes |  |  |  |  |  | 15540229
TRPC4 |  | ZO-1 | Co-immunoprecipitation | Human fetal brain astrocytes |  |  |  |  |  | 15540229

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 694-718 | Bovine | Not determined |  | 94 nM | 11311128
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 829-853 | Bovine | Not determined |  | 138 nM | 11311128
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 708-728 | Bovine | Not determined |  | 189 nM | 11311128
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 695-724 | Not specified | Not determined |  | 200 nM | 11290752
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 787-812 | Not specified | Not determined |  | 600 nM | 11290752
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 786-848 | Not specified | Not determined |  |  | 11311128
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 688-759 | Not specified | Not determined |  |  | 11311128
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 695-724 | Not specified | Not determined |  |  | 15104175
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 789-812 | Not specified | Not determined |  |  | 11290752
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 695-724 | Not specified | Not determined |  |  | 11290752
TRPC4 |  | Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 61-101 |  |  | 17416589
TRPC4 |  | Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 135-178 |  |  | 17416589
TRPC4 |  | D2R | Yeast two-hybrid | Human | 712-731 | Human | 131-151 |  |  | 18261457
TRPC4 |  | Gơi2 | Co-immunoprecipitation | Mouse | 700-728 | Human | Not determined |  |  | 22457348
TRPC4 |  | ƢV-spectrin | Fusion protein-pull down assay | Human | 686-977 | Human | 3232-3674 |  |  | 18048348
TRPC4 |  | ƢV-spectrin | Yeast two-hybrid | Human | 686-977 | Human | 3232-3674 |  |  | 18048348
TRPC4 |  | IP3R2 | Fusion protein-pull down assay | Human | 785-868 | Rat | 2556-2713 |  |  | 11163362
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 |  |  | 15104175
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 781-864 | Human | 669-698 |  |  | 11290752
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 |  |  | 11290752
TRPC4 |  | MxA | Fusion protein-pull down assay | Human | 3-183 | Human | 572-662 |  |  | 15757897
TRPC4 |  | NHERF1 | Co-immunoprecipitation | Human | 975-977 (in ơ varint) | Human | 130-328 |  |  | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | 975-977 (in ơ varint) | Human | 1-102 |  |  | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Human | 975-977 (in ơ varint) | Human | 144-247 |  |  | 12154080
TRPC4 |  | NHERF1 | Fusion protein-pull down assay | Mouse | 688-974 | Mouse | 1-107 |  |  | 10980202
TRPC4 |  | ơ1-syntrophin | Fusion protein-pull down assay | Mouse | Not determined | Mouse | 75-170 |  |  | 19812031
TRPC4 |  | ơII-spectrin | Fusion protein-pull down assay | Human | 730-758 | Human | 2202-2313 |  |  | 18048348
TRPC4 |  | ơII-spectrin | Yeast two-hybrid | Human | 686-859 | Human | 1998-2472 |  |  | 18048348
TRPC4 |  | PLCƣ1 | Yeast two-hybrid | Human | 1-330 | Rat | 780-920 |  |  | 12437926
TRPC4 |  | SESTD1 | Fusion protein-pull down assay | Mouse | 700-728 | Human | 193-406 |  |  | 20164195
TRPC4 |  | STIM1 | Co-immunoprecipitation | Mouse | Not determined | Human | 251-535 |  |  | 16906149
TRPC4 |  | TARBP2 | Co-immunoprecipitation | Mouse | 625-974 | Mouse | 209-234 |  |  | 24563462
TRPC4 |  | TRPC5 | Fluorescence resonance energy transfer | Mouse | 1-292 | Mouse | 1-308 |  |  | 17624425
TRPC4 |  | TRPC6 | Co-immunoprecipitation | Mouse | 1-304 | Mouse | 715-930 |  |  | 16916799
TRPC4 |  | ZO-1 | Fusion protein-pull down assay | Human | 890-892 | Not specified | First PDZ domain |  |  | 15540229

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC4 |  | Gơi2 | Patch clamp |  |  | Activation | 22457348
TRPC4 |  | Ƣ-catenin | Calcium measurement |  |  | Activation | 19996314
TRPC4 |  | NHERF1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 12154080
TRPC4 |  | ơII-spectrin | Cell surface biotinylation |  | Increase in plasma membrane level |  | 18048348
TRPC4 |  | Protein 4.1 | Calcium measurement |  |  | Activation | 16254212
TRPC4 |  | Protein 4.1 | Patch clamp |  |  | Activation | 16254212
TRPC4 |  | RNF24 | Cell surface biotinylation |  | Decrease in plasma membrane level |  | 17850865
TRPC4 |  | TRPC1 | Patch clamp |  |  | New channel creation | 11301024
TRPC4 |  | TRPC1 | Patch clamp |  |  | New channel creation | 22144671

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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