# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPM7 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (13)
- Biological Process (4)
- Disease (1)

Name: Transient receptor potential cation channel subfamily M member 7 (TRPM7)
Official Symbol: TRPM7 provided by HGNC
Function:
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 54822 | Q96QT4 | DIP-47486N |  | MINT-2815126 | 120177 | Q96QT4 | 499 | hsa:54822 | 605692
Mouse | 58800 | Q923J1 | DIP-43725N |  | MINT-2524821 | 208438 | Q923J1 |  | mmu:58800 |
Rat | 679906 | Q925B3 | DIP-43727N |  | MINT-2778884 | 594845 | Q925B3 |  | rno:679906 |

PPI pairs:

Annexin 1 | Myosin-HIIA | PLCƢ3 | PLCƣ1 | PLCƣ2 | PLCƤ1 | RACK1 | SNAPIN
Synapsin1 | Synaptotagmin 1 | TRPM6 | UBC | ƣ-actin

Biological Process:

- GO:0031032 : actomyosin structure organization
- GO:0016340 : calcium-dependent cell-matrix adhesion
- GO:0008219 : cell death
- GO:0046777 : protein autophosphorylation

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:605692 : Amyotrophic lateral sclerosis-parkinsonism/dementia complex 1, susceptibility to (OMIM:105500 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM7 |  | Annexin 1 | Inference |  |  |  |  |  | Prediction | 15485879
TRPM7 |  | Ƣ-actin | Inference |  |  |  |  |  | Prediction | 16407977
TRPM7 |  | Myosin-HIIA | Inference |  |  |  |  |  | Prediction | 16407977
TRPM7 |  | PLCƣ1 | Inference |  |  |  |  |  | Prediction | 11941371
TRPM7 |  | PLCƢ1 | Yeast two-hybrid | Not used as a bait |  | Rat | Brain | cDNA library |  | 11161216
TRPM7 |  | PLCƣ2 | Inference |  |  |  |  |  | Prediction | 22759789
TRPM7 |  | PLCƢ2 | Inference |  |  |  |  |  | Prediction | 11941371
TRPM7 |  | PLCƢ3 | Inference |  |  |  |  |  | Prediction | 11941371
TRPM7 |  | PLCƤ1 | Inference |  |  |  |  |  | Prediction | 22180838
TRPM7 |  | RacK1 | Inference |  |  |  |  |  | Prediction | 18258429
TRPM7 |  | SNAPIN | Yeast two-hybrid | Human | 87-326 | Human | Brain | cDNA library |  | 17088214
TRPM7 |  | Synapsin1 | Inference |  |  |  |  |  | Prediction | 17088214
TRPM7 |  | Synaptotagmin 1 | Inference |  |  |  |  |  | Prediction | 17088214
TRPM7 |  | TRPM6 | Inference |  |  |  |  |  | Prediction | 14976260
TRPM7 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | HEK293T | Cell lystaes |  | 21906983

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM7 |  | PLCƢ1 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371
TRPM7 |  | PLCƢ1 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM7 |  | PLCƢ1 | Yeast two-hybrid | Rat | 1532-1862 | Yeast | Rat | 643-1216 | Yeast | 11941371
TRPM7 |  | PLCƢ2 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371
TRPM7 |  | PLCƢ2 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM7 |  | PLCƢ3 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371
TRPM7 |  | PLCƢ3 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM7 |  | PLCƤ1 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM7 |  | RacK1 | Fusion protein-pull down assay | Mouse | ơ-kinase domain | E. coli | Mouse | Full-length | In vitro translation | 18258429
TRPM7 |  | SNAPIN | Fusion protein-pull down assay | Human | 83-326 | In vitro translation | Human | Full-length | Not specified | 17088214

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM7 |  | Annexin 1 | Co-immunofluorescence staining |  | HEK293 | Human | ChaK1 domain | Human | Not determined | 24939696
TRPM7 |  | Annexin 1 | Co-immunoprecipitation |  | HEK293 | Human | ChaK1 domain | Human | Not determined | 24939696
TRPM7 |  | Ƣ-actin | Co-immunofluorescence staining |  | N1E-115 neuroblastoma cell | Mouse | Full-length | Not used |  | 16407977
TRPM7 |  | Ƣ-actin | Co-immunoprecipitation |  | N1E-115 neuroblastoma cell | Mouse | Full-length | Not used |  | 16407977
TRPM7 |  | Myosin-HIIA | Co-immunoprecipitation |  | N1E-115 neuroblastoma cell | Mouse | Full-length | Not used |  | 16407977
TRPM7 |  | Myosin-HIIA | Co-immunoprecipitation | PC12 |  |  |  |  |  | 16407977
TRPM7 |  | PLCƣ2 | Co-immunoprecipitation |  | DT40 | Mouse | Full-length | Not used |  | 22759789
TRPM7 |  | PLCƢ2 | Co-immunoprecipitation |  | HEK293T | Rat | Full-length | Rat | Full-length | 11941371
TRPM7 |  | SNAPIN | Co-immunoprecipitation | Rat brain synaptic vesicle |  |  |  |  |  | 17088214
TRPM7 |  | Synapsin1 | Co-immunofluorescence staining | Rat SCG sympathetic neuron |  |  |  |  |  | 17088214
TRPM7 |  | Synapsin1 | Co-immunoprecipitation | Rat brain synaptic vesicle |  |  |  |  |  | 17088214
TRPM7 |  | Synaptotagmin 1 | Co-immunoprecipitation | Rat brain synaptic vesicle |  |  |  |  |  | 17088214
TRPM7 |  | TRPM6 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 14976260

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM7 |  | Annexin 1 | In vitro PTM assay | Human | ChaK1 domain | Human | Not determined |  |  | 15485879
TRPM7 |  | Annexin 1 | In vitro PTM assay | Human | ChaK1 domain | Bovine | Not determined |  |  | 15485879
TRPM7 |  | Myosin-HIIA | Co-immunoprecipitation | Mouse | 1158-1864 | Mouse | 1795-1960 |  |  | 16407977
TRPM7 |  | PLCƢ1 | Yeast two-hybrid | Rat | 1532-1862 | Rat | 643-797 |  |  | 11941371
TRPM7 |  | RacK1 | Fusion protein-pull down assay | Mouse | ơ-kinase domain | Mouse | Not determined |  |  | 18258429
TRPM7 |  | SNAPIN | Fusion protein-pull down assay | Human | 87-326 | Human | 43-68 |  |  | 17088214
TRPM7 |  | TRPM6 | Fusion protein-pull down assay | Mouse | 1548-1863 | Mouse | 1711-2028 |  |  | 19228120

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM7 |  | PLCƢ1 | Patch clamp |  |  | Inhibition | 11941371
TRPM7 |  | PLCƢ2 | Patch clamp |  |  | Inhibition | 11941371
TRPM7 |  | PLCƢ3 | Patch clamp |  |  | Inhibition | 11941371
TRPM7 |  | RacK1 | Patch clamp |  |  | Inhibition | 18258429
TRPM7 |  | TRPM6 | Patch clamp |  |  | New channel creation | 24385424

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
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