# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# SYNE1 / TRP channels

- Basic Information
- External Database
- PPI pairs (1)
- Biological Process (5)
- Disease (2)

Name: Nesprin-1 (SYNE1)
Official Symbol: SYNE1 provided by HGNC
Function:
Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. May be involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. May be required for centrosome migration to the apical cell surface during early ciliogenesis.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 23345 | Q8NF91 | DIP-44163N | Q8NF91 | MINT-6489007 | 116928 | Q8NF91 |  | hsa:23345 | 608441
Mouse | 64009 | Q6ZWR6 |  |  |  | 211015 | Q6ZWR6 |  | mmu:64009 |
Rat | 499010 | Q8VHJ9 |  |  |  | 270319 | Q8VHJ9 |  | rno:499010 |

PPI pairs:

TRPV2

Biological Process:

- GO:0008219 : cell death
- GO:0090286 : cytoskeletal anchoring at nuclear membrane
- GO:0007030 : Golgi organization
- GO:0042692 : muscle cell differentiation
- GO:0090292 : nuclear matrix anchoring at nuclear membrane

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:608441 : Spinocerebellar ataxia, autosomal recessive 8 (OMIM:610743 )
- OMIM:608441 : Emery-Dreifuss muscular dystrophy 4, autosomal dominant (OMIM:612998 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV2 |  | SYNE1 | Yeast two-hybrid | Mouse | 1-167 | Human | Heart | cDNA library |  | 16875688

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV2 |  | SYNE1 | Fusion protein-pull down assay | Not used |  | Rat heart lysates | Human | 1-476 (in GSRP-56 Variant) | E. coli | 16875688

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV2 |  | SYNE1 | Fusion protein-pull down assay | Rat | Not determined | Human | 1-258 (in GSRP-56 Variant) |  |  | 16875688
TRPV2 |  | SYNE1 | Fusion protein-pull down assay | Rat | Not determined | Human | 1-258 |  |  | 16875688

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.