# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Orai1 / TRP channels

- Basic Information
- External Database
- PPI pairs (4)
- Biological Process (3)
- Disease (1)

Name: Calcium release-activated calcium channel protein 1 (Orai1)
Official Symbol: ORAI1 provided by HGNC
Function:
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 84876 | Q96D31 | DIP-46289N | Q96D31 | MINT-6780078 | 124321 | Q96D31 |  | hsa:84876 | 610277
Mouse | 109305 | Q8BWG9 |  |  |  | 224640 | Q8BWG9 |  | mmu:109305 |
Rat | 304496 | Q5M848 |  |  |  | 257881 | Q5M848 |  | rno:304496 |

PPI pairs:

TRPA1 | TRPC1 | TRPC3 | TRPC6

Biological Process:

- GO:0007596 : blood coagulation
- GO:0051928 : positive regulation of calcium ion transport
- GO:0002115 : store-operated calcium entry

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:610277 : Immune dysfunction with T-cell inactivation due to calcium entry defect 1 (OMIM:612782 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPA1 |  | Orai1 | Inference |  |  |  |  |  | Prediction | 23994313
TRPC1 |  | Orai1 | Inference |  |  |  |  |  | Prediction | 18326500
TRPC3 |  | Orai1 | Inference |  |  |  |  |  | Prediction | 17360584
TRPC6 |  | Orai1 | Inference |  |  |  |  |  | Prediction | 17360584

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC3 |  | Orai1 | Fusion protein-pull down assay | Human | 1-340 | In vitro translation | Human | Full-length | E. coli | 17360584
TRPC3 |  | Orai1 | Fusion protein-pull down assay | Human | 676-848 | In vitro translation | Human | Full-length | E. coli | 17360584
TRPC6 |  | Orai1 | Fusion protein-pull down assay | Mouse | 1-396 | In vitro translation | Human | Full-length | E. coli | 17360584
TRPC6 |  | Orai1 | Fusion protein-pull down assay | Mouse | 732-930 | In vitro translation | Human | Full-length | E. coli | 17360584

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPA1 |  | Orai1 | Co-immunoprecipitation | MEG-01 |  |  |  |  |  | 23994313
TRPC1 |  | Orai1 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Human | Full-length | 23770672
TRPC1 |  | Orai1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 18326500
TRPC1 |  | Orai1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 18644792
TRPC1 |  | Orai1 | Co-immunoprecipitation | Human atrial myocyte |  |  |  |  |  | 23686296
TRPC1 |  | Orai1 | Co-immunoprecipitation | Human parathyroid tissue |  |  |  |  |  | 20194530
TRPC3 |  | Orai1 | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Human | Full-length | 17360584
TRPC3 |  | Orai1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 20022948
TRPC6 |  | Orai1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 19260825
TRPC6 |  | Orai1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 17360584

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC3 |  | Orai1 | Patch clamp |  |  | Activation | 18287061

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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