# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# PACS-1 / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (6)
- Disease (0)

Name: Phosphofurin acidic cluster sorting protein 1 (PACS-1)
Official Symbol: PACS1 provided by HGNC
Function:
Coat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 55690 | Q6VY07 | DIP-42824N | Q6VY07 | MINT-3372090 | 120816 | Q6VY07 |  | hsa:55690 | 607492
Mouse | 107975 | Q8K212 | DIP-37699N | Q8K212 | UNK-5520352 | 223732 | Q8K212 |  | mmu:107975 |
Rat | 171444 | O88588 | DIP-42823N |  | MINT-3372040 | 251254 | O88588 |  | rno:171444 |

PPI pairs:

TRPP1 | TRPV1 | TRPV4

Biological Process:

- GO:0032092 : positive regulation of protein binding
- GO:0000042 : protein targeting to Golgi
- GO:0072661 : protein targeting to plasma membrane
- GO:0050690 : regulation of defense response to virus by virus
- GO:0016032 : viral reproduction
- GO:0019048 : virus-host interaction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPP1 |  | PACS-1 | Inference |  |  |  |  |  | Prediction | 15692563
TRPV1 |  | PACS-1 | Inference |  |  |  |  |  | Prediction | 23159249
TRPV4 |  | PACS-1 | Inference |  |  |  |  |  | Prediction | 15692563

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPP1 |  | PACS-1 | Fusion protein-pull down assay | Human | Full-length | HEK293T lysates | Human | Full-length | Not specified | 15692563
TRPP1 |  | PACS-1 | Fusion protein-pull down assay | Not used |  | Mouse kidney tissue lysates | Human | Full-length | Not specified | 15692563

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | PACS-1 | Co-immunoprecipitation | COS M6 kidney cell |  |  |  |  |  | 15692563
TRPV1 |  | PACS-1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 23159249
TRPV4 |  | PACS-1 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15692563

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | PACS-1 | Fluorescence probe labeling |  |  |  |  |  | 14.8 ƬM | 15692563
TRPP1 |  | PACS-1 | Fusion protein-pull down assay | Human | 741-871 | Human | 115-255 |  |  | 15692563
TRPV4 |  | PACS-1 | Co-immunoprecipitation | Human | 1-486 | Human | Not determined |  |  | 15692563

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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