# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPM8 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (5)
- Biological Process (5)
- Disease (0)

Name: Transient receptor potential cation channel subfamily M member 8 (TRPM8)
Official Symbol: TRPM8 provided by HGNC
Function:
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 79054 | Q7Z2W7 |  |  |  | 122512 | Q7Z2W7 | 500 | hsa:79054 | 606678
Mouse | 171382 | Q8R4D5 |  |  |  | 228590 | Q8R4D5 |  | mmu:171382 |
Rat | 171384 | Q8R455 |  |  |  | 251219 | Q8R455 |  | rno:171384 |

PPI pairs:

5-HT1B | AKAP5/AKAP150 | Gơq | OPRM1 | PIRT

Biological Process:

- GO:0006874 : cellular calcium ion homeostasis
- GO:0051289 : protein homotetramerization
- GO:0070207 : protein homotrimerization
- GO:0009409 : response to cold
- GO:0050955 : thermoception

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM8 |  | 5-HT1B | Inference |  |  |  |  |  | Prediction | 24269608
TRPM8 |  | AKAP5/AKAP150 | Speculation |  |  |  |  |  | Prediction | 18701070
TRPM8 |  | Gơq | Inference |  |  |  |  |  | Prediction | 22460725
TRPM8 |  | OPRM1 | Inference |  |  |  |  |  | Prediction | 23911290
TRPM8 |  | PIRT | Inference |  |  |  |  |  | Prediction | 18455988

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM8 |  | Gơq | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | Full-length | HEK293 lysates | 22750945
TRPM8 |  | Gơq | Fusion protein-pull down assay | Human | Full-length | E. coli | Human | Full-length | HEK293 lysates | 22750945

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM8 |  | 5-HT1B | Co-immunofluorescence staining | Rat dorsal root ganglion neuron |  |  |  |  |  | 24269608
TRPM8 |  | 5-HT1B | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 24269608
TRPM8 |  | AKAP5/AKAP150 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Not used |  | 18701070
TRPM8 |  | Gơq | Co-immunofluorescence staining |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 22460725
TRPM8 |  | Gơq | Co-immunoprecipitation | Mouse dorsal root ganglion cell |  |  |  |  |  | 22750945
TRPM8 |  | Gơq | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Not used |  | 23508958
TRPM8 |  | Gơq | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 22460725
TRPM8 |  | OPRM1 | Co-immunofluorescence staining | Rat dorsal root ganglion neuron |  |  |  |  |  | 23911290
TRPM8 |  | OPRM1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 23911290
TRPM8 |  | PIRT | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Mouse | Full-length | 18455988

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM8 |  | Gơq | Fusion protein-pull down assay | Human | 1-691 | Human | Not determined |  |  | 22750945
TRPM8 |  | Gơq | Fusion protein-pull down assay | Human | 980-1104 | Human | Not determined |  |  | 22750945
TRPM8 |  | Gơq | Fusion protein-pull down assay | Human | Not determined | Human | 222-245 |  |  | 22750945
TRPM8 |  | PIRT | Co-immunoprecipitation | Not specified | Not determined | Mouse | 110-135 |  |  | 18455988

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM8 |  | 5-HT1B | Calcium measurement |  |  | Activation | 24269608
TRPM8 |  | Gơq | Patch clamp |  |  | Inhibition | 22750945
TRPM8 |  | OPRM1 | Cell surface biotinylation |  | Internalization from membrane by ligand |  | 23911290

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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