# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# CD2AP / TRP channels

- Basic Information
- External Database
- PPI pairs (1)
- Biological Process (5)
- Disease (1)

Name: CD2-associated protein (CD2AP)
Official Symbol: CD2AP provided by HGNC
Function:
Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 23607 | Q9Y5K6 | DIP-31807N | Q9Y5K6 | MINT-93491 | 117140 | Q9Y5K6 |  | hsa:23607 | 604241
Mouse | 12488 | Q9JLQ0 | DIP-41585N | Q9JLQ0 | MINT-255809 | 198584 | Q9JLQ0 |  | mmu:12488 |
Rat | 316258 | Q80ZE7 |  |  |  | 261303 | Q80ZE7 |  | rno:316258 |

PPI pairs:

TRPP1

Biological Process:

- GO:0051301 : cell division
- GO:0007067 : mitosis
- GO:0006461 : protein complex assembly
- GO:0007165 : signal transduction
- GO:0006930 : substrate-dependent cell migration, cell extension

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:604241 : Glomerulosclerosis, focal segmental, 3 (OMIM:607832 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPP1 |  | CD2AP | Yeast two-hybrid | Mouse | 680-968 | Mouse | Embryonic | cDNA library |  | 10913159

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPP1 |  | CD2AP | Fusion protein-pull down assay | Mouse | 747-968 | E. coli | Mouse | 331-637 | E. coli | 10913159

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | CD2AP | Co-immunofluorescence staining | M-1 cell |  |  |  |  |  | 10913159
TRPP1 |  | CD2AP | Co-immunofluorescence staining |  | LLC-PK1 | Human | Full-length | Mouse | Full-length | 10913159
TRPP1 |  | CD2AP | Co-immunoprecipitation | M-1 cell |  |  |  |  |  | 10913159

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | CD2AP | Fusion protein-pull down assay | Mouse | 747-968 | Mouse | 331-637 |  |  | 10913159

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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