# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# KIF3A / TRP channels

- Basic Information
- External Database
- PPI pairs (1)
- Biological Process (12)
- Disease (0)

Name: Kinesin-like protein KIF3A (KIF3A)
Official Symbol: KIF3A provided by HGNC
Function:
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 11127 | Q9Y496 | DIP-33237N | Q9Y496 | MINT-3086349 | 116300 | Q9Y496 |  | hsa:11127 | 604683
Mouse | 16568 | P28741 | DIP-46318N | P28741 | UNK-5375320 | 200941 | P28741 |  | mmu:16568 |
Rat | 84392 | Q9WV62 |  |  | UNK-5559667 | 249935 | Q9WV62 |  | rno:84392 |

PPI pairs:

TRPP1

Biological Process:

- GO:0009952 : anterior/posterior pattern specification
- GO:0019886 : antigen processing and presentation of exogenous peptide antigen via MHC class II
- GO:0007596 : blood coagulation
- GO:0042384 : cilium assembly
- GO:0007368 : determination of left/right symmetry
- GO:0021904 : dorsal/ventral neural tube patterning
- GO:0007507 : heart development
- GO:0001701 : in utero embryonic development
- GO:0001822 : kidney development
- GO:0006996 : organelle organization
- GO:0072383 : plus-end-directed vesicle transport along microtubule
- GO:0007224 : smoothened signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPP1 |  | KIF3A | Inference |  |  |  |  |  | Prediction | 16950792

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPP1 |  | KIF3A | Fusion protein-pull down assay | Human | 682-968 | E. coli | Human | Full-length | In vitro translation | 16950792
TRPP1 |  | KIF3A | Yeast two-hybrid | Human | 682-968 | Yeast | Human | Full-length | Yeast | 16950792

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | KIF3A | Co-immunofluorescence staining | Mouse Inner medullary collecting duct cell |  |  |  |  |  | 16950792
TRPP1 |  | KIF3A | Co-immunofluorescence staining | MDCK |  |  |  |  |  | 16950792
TRPP1 |  | KIF3A | Co-immunofluorescence staining | LLC-PK1 |  |  |  |  |  | 16950792
TRPP1 |  | KIF3A | Co-immunoprecipitation | MDCK |  |  |  |  |  | 17283055

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | KIF3A | Fusion protein-pull down assay | Human | 682-968 | Human | 403-702 |  |  | 17008358
TRPP1 |  | KIF3A | Yeast two-hybrid | Human | 872-927 | Human | 403-702 |  |  | 16950792

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPP1 |  | KIF3A | Patch clamp |  |  | Activation | 16950792

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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