# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPM6 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (6)
- Biological Process (1)
- Disease (1)

Name: Transient receptor potential cation channel subfamily M member 6 (TRPM6)
Official Symbol: TRPM6 provided by HGNC
Function:
Essential ion channel and serine/threonine-protein kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Isoforms of the type M6-kinase lack the ion channel region.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 140803 | Q9BX84 | DIP-47487N |  | MINT-6699468 | 126713 | Q9BX84 | 498 | hsa:140803 | 607009
Mouse | 225997 | Q8CIR4 |  |  |  | 230456 | Q8CIR4 |  | mmu:225997 |
Rat | 293874 | A7L641 |  |  |  | 254495 | A7L641 |  | rno:293874 |

PPI pairs:

PLCƢ3 | PLCƣ1 | PLCƣ2 | RACK1 | REA | TRPM7

Biological Process:

- GO:0009636 : response to toxin

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:607009 : Hypomagnesemia 1, intestinal (OMIM:602014 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM6 |  | PLCƢ1 | Inference |  |  |  |  |  | Prediction | 22180838
TRPM6 |  | PLCƢ2 | Inference |  |  |  |  |  | Prediction | 22180838
TRPM6 |  | PLCƢ3 | Inference |  |  |  |  |  | Prediction | 22180838
TRPM6 |  | RacK1 | Yeast two-hybrid | Human | 1075-2022 | Mouse | Kidney | cDNA library |  | 18258429
TRPM6 |  | REA | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | Kidney | Tissue lysates |  | 19329436
TRPM6 |  | TRPM7 | Inference |  |  |  |  |  | Prediction | 14976260

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM6 |  | PLCƢ1 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM6 |  | PLCƢ2 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM6 |  | PLCƢ3 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838
TRPM6 |  | RacK1 | Yeast two-hybrid | Human | 1075-2022 | Yeast | Mouse | Full-length | Yeast | 18258429
TRPM6 |  | REA | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 19329436

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM6 |  | RacK1 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 18258429
TRPM6 |  | RacK1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not specified | Not specified | 18258429
TRPM6 |  | REA | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Mouse | Full-length | 19329436
TRPM6 |  | TRPM7 | Fluorescence resonance energy transfer |  | HEK293 | Human | Full-length | Mouse | Full-length | 14976260

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM6 |  | RacK1 | Fusion protein-pull down assay | Mouse | 1857-1885 | Mouse | Not determined |  |  | 18258429
TRPM6 |  | REA | Fusion protein-pull down assay | Mouse | 1857-1885 | Mouse | Not determined |  |  | 19329436
TRPM6 |  | TRPM7 | Fusion protein-pull down assay | Mouse | 1711-2028 | Mouse | 1548-1863 |  |  | 19228120

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM6 |  | RacK1 | Patch clamp |  |  | Inhibition | 18258429
TRPM6 |  | REA | Patch clamp |  |  | Inhibition | 19329436
TRPM6 |  | TRPM7 | Patch clamp |  |  | Activation | 14976260
TRPM6 |  | TRPM7 | Patch clamp |  |  | New channel creation | 24385424

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
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