# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Epo-R / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (5)
- Disease (1)

Name: Erythropoietin receptor (Epo-R)
Official Symbol: EPOR provided by HGNC
Function:
Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 2057 | P19235 | DIP-5732N | P19235 | MINT-273225 | 108371 | P19235 |  | hsa:2057 | 133171
Mouse | 13857 | P14753 | DIP-657N | P14753 | MINT-1348473 | 199488 | P14753 |  | mmu:13857 |
Rat | 24336 | Q07303 |  |  | MINT-4596642 | 246513 | Q07303 |  | rno:24336 |

PPI pairs:

TRPC2 | TRPC3 | TRPC6

Biological Process:

- GO:0007420 : brain development
- GO:0046697 : decidualization
- GO:0007204 : elevation of cytosolic calcium ion concentration
- GO:0009790 : embryo development
- GO:0007507 : heart development

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:133171 : Erythrocytosis, familial, 1 (OMIM:133100 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC2 |  | Epo-R | Inference |  |  |  |  |  | Prediction | 12167663
TRPC2 |  | Epo-R | Inference |  |  |  |  |  | Prediction | 15329338
TRPC3 |  | Epo-R | Inference |  |  |  |  |  | Prediction | 18276585
TRPC6 |  | Epo-R | Inference |  |  |  |  |  | Prediction | 19074769

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC2 |  | Epo-R | Co-immunofluorescence staining |  | CHO | Mouse | Full-length | Mouse | Full-length | 12167663
TRPC2 |  | Epo-R | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Mouse | Full-length | 15329338
TRPC2 |  | Epo-R | Co-immunoprecipitation | Mouse erythroblasts |  |  |  |  |  | 15329338
TRPC2 |  | Epo-R | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Mouse | Full-length | 22088247
TRPC3 |  | Epo-R | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 19074769
TRPC3 |  | Epo-R | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 21757714
TRPC3 |  | Epo-R | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 18276585
TRPC3 |  | Epo-R | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 19074769
TRPC3 |  | Epo-R | Co-immunoprecipitation | TF-1 |  |  |  |  |  | 19074769
TRPC6 |  | Epo-R | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 19074769

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | Epo-R | Co-immunoprecipitation | Human | 671-746 | Human | Not determined |  |  | 19074769

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC2 |  | Epo-R | Calcium measurement |  |  | Activation | 12167663
TRPC3 |  | Epo-R | Calcium measurement |  |  | Activation | 18276585
TRPC3 |  | Epo-R | Patch clamp |  |  | Activation | 18276585

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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