# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# MxA / TRP channels

- Basic Information
- External Database
- PPI pairs (6)
- Biological Process (6)
- Disease (0)

Name: Interferon-induced GTP-binding protein Mx1 (MxA)
Official Symbol: MX1 provided by HGNC
Function:
Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 4599 | P20591 | DIP-35694N | P20591 |  | 110684 | P20591 |  | hsa:4599 | 147150
Mouse | 17857 | P09922 |  |  |  | 201628 |  |  | mmu:17857 |
Rat | 24575 | P18588 |  |  |  | 246722 | P18588 |  | rno:24575 |

PPI pairs:

TRPC1 | TRPC3 | TRPC4 | TRPC5 | TRPC6 | TRPC7

Biological Process:

- GO:0051607 : defense response to virus
- GO:0006184 : GTP catabolic process
- GO:0006917 : induction of apoptosis
- GO:0045071 : negative regulation of viral genome replication
- GO:0009615 : response to virus
- GO:0060337 : type I interferon-mediated signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897
TRPC3 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897
TRPC4 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897
TRPC5 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897
TRPC6 |  | MxA | Yeast two-hybrid | Human | 3-259 | Human | Fetal brain | cDNA library |  | 15757897
TRPC7 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC3 |  | MxA | Fusion protein-pull down assay | Human | 1-201 | In vitro translation | Human | 572-662 | E. coli | 15757897
TRPC4 |  | MxA | Fusion protein-pull down assay | Human | 3-183 | In vitro translation | Human | 572-662 | E. coli | 15757897
TRPC5 |  | MxA | Fusion protein-pull down assay | Human | 1-183 | In vitro translation | Human | 572-662 | E. coli | 15757897
TRPC6 |  | MxA | Fusion protein-pull down assay | Human | 95-243 | In vitro translation | Human | 572-662 | E. coli | 15757897
TRPC7 |  | MxA | Fusion protein-pull down assay | Human | 3-195 | In vitro translation | Human | 572-662 | E. coli | 15757897

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | MxA | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15757897
TRPC3 |  | MxA | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15757897
TRPC4 |  | MxA | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15757897
TRPC6 |  | MxA | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15757897

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | MxA | Fusion protein-pull down assay | Human | 1-201 | Human | 572-662 |  |  | 15757897
TRPC4 |  | MxA | Fusion protein-pull down assay | Human | 3-183 | Human | 572-662 |  |  | 15757897
TRPC5 |  | MxA | Fusion protein-pull down assay | Human | 1-183 | Human | 572-662 |  |  | 15757897
TRPC6 |  | MxA | Fusion protein-pull down assay | Human | 95-243 | Human | 572-662 |  |  | 15757897
TRPC6 |  | MxA | Yeast two-hybrid | Human | 3-259 | Human | 542-662 |  |  | 15757897
TRPC7 |  | MxA | Fusion protein-pull down assay | Human | 3-195 | Human | 572-662 |  |  | 15757897

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC6 |  | MxA | Calcium measurement |  |  | Activation | 15757897

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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