# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Calmodulin / TRP channels

- Basic Information
- External Database
- PPI pairs (16)
- Biological Process (19)
- Disease (0)

Name: Calmodulin (Calmodulin)
Official Symbol: CALM1 provided by HGNC
Function:
Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 805 | P62158 | DIP-31794N | P62158 | MINT-4999725 | 107256 | P62158 |  | hsa:805 | 114180
Mouse | 12313 | P62204 | DIP-31550N | P62204 | MINT-1855685 | 198454 | P62204 |  | mmu:12313 |
Rat | 24242 | P62161 | DIP-29528N | P62161 | MINT-268601 | 246428 | P62161 |  | rno:24242 |

PPI pairs:

TRPC1 | TRPC2 | TRPC3 | TRPC4 | TRPC5 | TRPC6 | TRPC7 | TRPM2
TRPM3 | TRPM4 | TRPV1 | TRPV2 | TRPV3 | TRPV4 | TRPV5 | TRPV6

Biological Process:

- GO:0007202 : activation of phospholipase C activity
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0007186 : G-protein coupled receptor signaling pathway
- GO:0006006 : glucose metabolic process
- GO:0005980 : glycogen catabolic process
- GO:0006936 : muscle contraction
- GO:0060315 : negative regulation of ryanodine-sensitive calcium-release channel activity
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0046209 : nitric oxide metabolic process
- GO:0030168 : platelet activation
- GO:0002576 : platelet degranulation
- GO:0060316 : positive regulation of ryanodine-sensitive calcium-release channel activity
- GO:0032465 : regulation of cytokinesis
- GO:0050999 : regulation of nitric-oxide synthase activity
- GO:0010880 : regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
- GO:0051592 : response to calcium ion
- GO:0044281 : small molecule metabolic process
- GO:0007268 : synaptic transmission

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11983166
TRPC2 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC3 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11248050
TRPC4 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC5 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC6 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC7 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPM2 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 16461353
TRPM3 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 22451665
TRPM4 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 15590641
TRPV1 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 12808128
TRPV2 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 20686800
TRPV3 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 19864432
TRPV4 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 12724311
TRPV5 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 15123711
TRPV6 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11248124

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC1 |  | Calmodulin | Fusion protein-pull down assay | Human | 649-793 | E. coli | Not specified | Full-length | Not specified | 11983166
TRPC1 |  | Calmodulin | Fusion protein-pull down assay | Human | 1-341 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Not specified | Full-length | In vitro translation | 16153633
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-619 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC3 |  | Calmodulin | Fusion protein-pull down assay | Human | 1-340 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC3 |  | Calmodulin | Fusion protein-pull down assay | Human | 742-795 | E. coli | Rat | Full-length | In vitro translation | 11248050
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Not used |  | Bovine adrenal cortex lysates | Xenopus | Full-length | In vitro translation | 11311128
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-320 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Not specified | Not specified | Not specified | 11290752
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-331 | In vitro translation | Not specified | Not specified | Not specified | 15987684
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 701-807 | In vitro translation | Not specified | Not specified | Not specified | 15987684
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 762-975 | In vitro translation | Not specified | Not specified | Not specified | 15987684
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-321 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC6 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 828-883 | E. coli | Not specified | Full-length | In vitro translation | 12379180
TRPC6 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-396 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPM3 |  | Calmodulin | Fluorescence probe labeling | Human | 35-124 | E. coli | Not specified | Not specified | Not specified | 22451665
TRPM3 |  | Calmodulin | Fluorescence probe labeling | Human | 291-382 | E. coli | Not specified | Not specified | Not specified | 22451665
TRPV1 |  | Calmodulin | Fast performance liquid chromatography | Rat | 759-802 | E. coli | Human | Full-length | E. coli | 23109716
TRPV1 |  | Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | E. coli | Mouse | Full-length | E. coli | 23119017
TRPV1 |  | Calmodulin | Fusion protein-pull down assay | Rat | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 14699077
TRPV1 |  | Calmodulin | Fusion protein-pull down assay | Rat | C-terminus | E. coli | Bovine | Full-length | Not specified | 12808128
TRPV1 |  | Calmodulin | X-ray diffraction-structural analysis | Rat | 784-798 | E. coli | Human | Full-length | E. coli | 23109716
TRPV2 |  | Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined | Not specified | E. coli | 20926660
TRPV3 |  | Calmodulin | Fusion protein-pull down assay | Mouse | ARD domain | Not specified | Not determined | Not specified | In vitro translation | 20926660
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Not specified | Not specified | In vitro translation | 22492541
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | 718-871 | E. coli | Not used |  | HEK293T lysates | 21837266
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | C-terminus | E. coli | Not specified | Full-length | Not specified | 12724311
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-327 | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 578-730 | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Human | 584-725 | In vitro translation | Bovine | Full-length | E. coli | 11248124

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | Calmodulin | Co-immunoprecipitation | Mouse skeletal myotube lysates |  |  |  |  |  | 18215135
TRPC6 |  | Calmodulin | Co-immunoprecipitation | PC12D |  |  |  |  |  | 15994335
TRPM2 |  | Calmodulin | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Not specified | Full-length | 16461353
TRPV1 |  | Calmodulin | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Rat | Full-length | 12808128
TRPV6 |  | Calmodulin | Co-immunoprecipitation |  | Xenopus oocyte | Rabbit | Full-length | Not specified | Full-length | 15123711
TRPV6 |  | Calmodulin | Fluorescence resonance energy transfer |  | HEK293 | Rat | Full-length | Not specified | Full-length | 16959851

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | Calmodulin | Fluorescence probe labeling | Human | 720-749 | Not specified | Not determined |  | 30 nM | 11290752
TRPC1 |  | Calmodulin | Fusion protein-pull down assay | Human | 719-751 | Not specified | Not determined |  |  | 11983166
TRPC1 |  | Calmodulin | Fusion protein-pull down assay | Human | 758-793 | Not specified | Not determined |  |  | 11983166
TRPC1 |  | Calmodulin | Fusion protein-pull down assay | Human | 719-751 | Not specified | Not determined |  |  | 11290752
TRPC2 |  | Calmodulin | Fluorescence probe labeling | Mouse | 905-934 | Not specified | Not determined |  | 10 nM | 11290752
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 104-195 | Not specified | Not determined |  |  | 12601176
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 901-936 | Not specified | Not determined |  |  | 11290752
TRPC3 |  | Calmodulin | Fluorescence probe labeling | Human | 764-793 | Bovine | Not determined |  | 100 nM | 11248050
TRPC3 |  | Calmodulin | Fluorescence probe labeling | Human | 764-793 | Not specified | Not determined |  | 100 nM | 11290752
TRPC3 |  | Calmodulin | Fusion protein-pull down assay | Human | 761-795 | Not specified | Not determined |  |  | 11290752
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 694-718 | Bovine | Not determined |  | 94 nM | 11311128
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 829-853 | Bovine | Not determined |  | 138 nM | 11311128
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 708-728 | Bovine | Not determined |  | 189 nM | 11311128
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 695-724 | Not specified | Not determined |  | 200 nM | 11290752
TRPC4 |  | Calmodulin | Fluorescence probe labeling | Mouse | 787-812 | Not specified | Not determined |  | 600 nM | 11290752
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 786-848 | Not specified | Not determined |  |  | 11311128
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 688-759 | Not specified | Not determined |  |  | 11311128
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 695-724 | Not specified | Not determined |  |  | 15104175
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 789-812 | Not specified | Not determined |  |  | 11290752
TRPC4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 695-724 | Not specified | Not determined |  |  | 11290752
TRPC5 |  | Calmodulin | Fluorescence probe labeling | Mouse | 702-731 | Not specified | Not determined |  | 220 nM | 11290752
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 701-733 | Bovine | Not determined |  |  | 15987684
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 828-854 | Bovine | Not determined |  |  | 15987684
TRPC5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 701-733 | Not specified | Not determined |  |  | 11290752
TRPC6 |  | Calmodulin | Fluorescence probe labeling |  |  |  |  |  | 320 nM | 19932145
TRPC6 |  | Calmodulin | Fluorescence probe labeling | Mouse | 843-872 | Not specified | Not determined |  | 290 nM | 11290752
TRPC6 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 838-872 | Not specified | Not determined |  |  | 11290752
TRPC7 |  | Calmodulin | Fluorescence probe labeling | Mouse | 769-798 | Not specified | Not determined |  | 19 nM | 11290752
TRPC7 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 766-798 | Not specified | Not determined |  |  | 11290752
TRPM2 |  | Calmodulin | Co-immunoprecipitation | Human | 1-730 | Not specified | Not determined |  |  | 16461353
TRPM2 |  | Calmodulin | Co-immunoprecipitation | Human | 406-416 | Not specified | Not determined |  |  | 16461353
TRPM2 |  | Calmodulin | Co-immunoprecipitation | Human | 1060-1503 | Not specified | Not determined |  |  | 16461353
TRPM3 |  | Calmodulin | Fluorescence probe labeling | Human | 291-382 | Not specified | Not determined |  |  | 22451665
TRPM3 |  | Calmodulin | Fluorescence probe labeling | Human | 35-124 | Not specified | Not determined |  |  | 22451665
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 5-25 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 186-212 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 1076-1098 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 1146-1167 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 1095-1151 | Not specified | Not determined |  |  | 15590641
TRPV1 |  | Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | Mouse | Not determined |  |  | 23119017
TRPV1 |  | Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV1 |  | Calmodulin | Fusion protein-pull down assay | Rat | 189-222 | Not specified | Not determined |  |  | 14699077
TRPV1 |  | Calmodulin | Fusion protein-pull down assay | Human | 101-364 | Not specified | Not determined |  |  | 19864432
TRPV1 |  | Calmodulin | Fusion protein-pull down assay | Rat | 767-801 | Bovine | Not determined |  |  | 12808128
TRPV1 |  | Calmodulin | X-ray diffraction-structural analysis | Rat | 784-798 | Human | 11-145 |  |  | 23109716
TRPV2 |  | Calmodulin | Fluorescence probe labeling | Human | 654-683 | Not specified | Not determined |  | 191 nM | 20686800
TRPV2 |  | Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined |  |  | 20926660
TRPV3 |  | Calmodulin | Fusion protein-pull down assay | Mouse | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV3 |  | Calmodulin | Fusion protein-pull down assay | Human | 115-367 | Not specified | Not determined |  |  | 19864432
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Chicken | 132-383 | Not specified | Not determined |  |  | 19864432
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | 718-871 | Human | Not determined |  |  | 21837266
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | 812-831 | Not specified | Not determined |  |  | 12724311
TRPV5 |  | Calmodulin | Fluorescence probe labeling | Human | 587-616 | Not specified | Not determined |  | 306 nM | 20686800
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Human | 696-729 | Not specified | Not determined |  |  | 21576356
TRPV5 |  | Calmodulin | Native-gel electrophoresis | Human | 696-729 | Not specified | Not determined |  |  | 21576356
TRPV6 |  | Calmodulin | Fluorescence probe labeling |  |  |  |  | 1:1 | 43 nM | 12765696
TRPV6 |  | Calmodulin | Fluorescence probe labeling |  |  |  |  |  | 65 nM | 11248124
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | 327-577 | Not specified | Not determined |  |  | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | 88-97 | Not specified | Not determined |  |  | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | 643-656 | Not specified | Not determined |  |  | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Human | 691-711 | Bovine | Not determined |  |  | 11248124

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC1 |  | Calmodulin | Calcium measurement |  |  | Inhibition | 11983166
TRPC1 |  | Calmodulin | Patch clamp |  |  | Inhibition | 11983166
TRPC3 |  | Calmodulin | Patch clamp |  |  | Inhibition | 11248050
TRPC6 |  | Calmodulin | Calcium measurement |  |  | Activation | 12379180
TRPC6 |  | Calmodulin | Patch clamp |  |  | Activation | 12379180
TRPM2 |  | Calmodulin | Calcium measurement |  |  | Activation | 16461353
TRPM4 |  | Calmodulin | Patch clamp |  |  | Activation | 15590641
TRPV1 |  | Calmodulin | Patch clamp |  |  | Inhibition | 14699077
TRPV1 |  | Calmodulin | Patch clamp |  |  | Sensitization | 23109716
TRPV1 |  | Calmodulin | Patch clamp |  |  | Inhibition | 12808128
TRPV4 |  | Calmodulin | Patch clamp |  |  | Activation | 12724311
TRPV5 |  | Calmodulin | Calcium measurement |  |  | Inhibition | 21576356
TRPV5 |  | Calmodulin | Patch clamp |  |  | Inhibition | 21576356
TRPV6 |  | Calmodulin | Calcium measurement |  |  | Inhibition | 16959851
TRPV6 |  | Calmodulin | Patch clamp |  |  | Inhibition | 15123711
TRPV6 |  | Calmodulin | Patch clamp |  |  | Inhibition | 11248124

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.