# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# IP3R1 / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (11)
- Disease (1)

Name: Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1)
Official Symbol: ITPR1 provided by HGNC
Function:
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 3708 | Q14643 | DIP-29714N | Q14643 | MINT-4991320 | 109913 | Q14643 |  | hsa:3708 | 147265
Mouse | 16438 | P11881 | DIP-32243N | P11881 | MINT-4099099 | 200847 | P11881 |  | mmu:16438 |
Rat | 25262 | P29994 | DIP-29715N |  | MINT-5026585 | 247302 | P29994 |  | rno:25262 |

PPI pairs:

TRPC3 | TRPC4 | TRPP1

Biological Process:

- GO:0007202 : activation of phospholipase C activity
- GO:0006112 : energy reserve metabolic process
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0070059 : intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0050796 : regulation of insulin secretion
- GO:0051209 : release of sequestered calcium ion into cytosol
- GO:0001666 : response to hypoxia
- GO:0044281 : small molecule metabolic process

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:147265 : Spinocerebellar ataxia 15 (OMIM:606658 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC3 |  | IP3R1 | Affinity purification-mass spectrometry | Not used as a bait |  | Rat | Brain | Crude membranes |  | 18205297
TRPC3 |  | IP3R1 | Inference |  |  |  |  |  | Prediction | 10518223
TRPC4 |  | IP3R1 | Inference |  |  |  |  |  | Prediction | 11163362
TRPP1 |  | IP3R1 | Affinity purification-mass spectrometry | Mouse | Not specified | Mouse | Vascular smooth muscle | MOVAS lysates |  | 19879844
TRPP1 |  | IP3R1 | Inference |  |  |  |  |  | Prediction | 16223735

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC4 |  | IP3R1 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2520-2670 | Yeast | 11163362

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | IP3R1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 11524429
TRPC3 |  | IP3R1 | Co-immunoprecipitation | Rat cerebral arterial lysates |  |  |  |  |  | 20378853
TRPC3 |  | IP3R1 | Co-immunoprecipitation | Rat mesenteric artery lysates |  |  |  |  |  | 23045459
TRPC3 |  | IP3R1 | Co-immunoprecipitation | Rat brain lysates |  |  |  |  |  | 18205297
TRPC3 |  | IP3R1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Rat | Full-length | 10518223
TRPC3 |  | IP3R1 | Co-immunoprecipitation |  | MDCK | Not specified | Full-length | Not used |  | 15623527
TRPC3 |  | IP3R1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 20022948
TRPP1 |  | IP3R1 | Co-immunofluorescence staining |  | LLC-PK1 | Human | Full-length | Not used |  | 20375013
TRPP1 |  | IP3R1 | Co-immunoprecipitation |  | COS-7 | Human | Full-length | Not used |  | 16223735
TRPP1 |  | IP3R1 | Co-immunoprecipitation |  | Xenopus oocyte | Human | 1-741 | Not used |  | 16223735
TRPP1 |  | IP3R1 | Co-immunoprecipitation |  | Xenopus oocyte | Human | Full-length | Not used |  | 16223735
TRPP1 |  | IP3R1 | Co-immunoprecipitation |  | LLC-PK1 | Human | Full-length | Not used |  | 20375013

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | IP3R1 | Co-immunoprecipitation | Human | Not determined | Rat | IP3 binding domain |  |  | 10518223
TRPC3 |  | IP3R1 | Fusion protein-pull down assay | Mouse | 725-848 | Not specified | 654-688 |  |  | 11290752
TRPP1 |  | IP3R1 | Fusion protein-pull down assay | Human | Not determined | Human | 1-345 |  |  | 20375013

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC3 |  | IP3R1 | Calcium measurement |  |  | Activation | 10518223
TRPC3 |  | IP3R1 | Patch clamp |  |  | Activation | 10518223

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.