# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# D2R / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (73)
- Disease (1)

Name: D(2) dopamine receptor (D2R)
Official Symbol: DRD2 provided by HGNC
Function:
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 1813 | P14416 | DIP-5977N | P14416 | MINT-201447 | 108147 | P14416 |  | hsa:1813 | 126450
Mouse | 13489 | P61168 |  |  | MINT-5313868 | 199306 | P61168 |  | mmu:13489 |
Rat | 24318 | P61169 | DIP-30883N | P61169 | MINT-4792453 | 246498 | P61169 |  | rno:24318 |

PPI pairs:

TRPC1 | TRPC4 | TRPC5

Biological Process:

- GO:0032147 : activation of protein kinase activity
- GO:0021984 : adenohypophysis development
- GO:0007195 : adenylate cyclase-inhibiting dopamine receptor signaling pathway
- GO:0007628 : adult walking behavior
- GO:0050482 : arachidonic acid secretion
- GO:0031223 : auditory behavior
- GO:0007409 : axonogenesis
- GO:0048148 : behavioral response to cocaine
- GO:0048149 : behavioral response to ethanol
- GO:0048755 : branching morphogenesis of a nerve
- GO:0021853 : cerebral cortex GABAergic interneuron migration
- GO:0032922 : circadian regulation of gene expression
- GO:0042417 : dopamine metabolic process
- GO:0051482 : elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
- GO:0007631 : feeding behavior
- GO:0002031 : G-protein coupled receptor internalization
- GO:0007625 : grooming behavior
- GO:0007243 : intracellular protein kinase cascade
- GO:0007616 : long-term memory
- GO:0007194 : negative regulation of adenylate cyclase activity
- GO:0045776 : negative regulation of blood pressure
- GO:0030336 : negative regulation of cell migration
- GO:0008285 : negative regulation of cell proliferation
- GO:0042321 : negative regulation of circadian sleep/wake cycle, sleep
- GO:0060160 : negative regulation of dopamine receptor signaling pathway
- GO:0046676 : negative regulation of insulin secretion
- GO:0051898 : negative regulation of protein kinase B signaling cascade
- GO:0050709 : negative regulation of protein secretion
- GO:0051967 : negative regulation of synaptic transmission, glutamatergic
- GO:1901386 : negative regulation of voltage-gated calcium channel activity
- GO:0001976 : neurological system process involved in regulation of systemic arterial blood pressure
- GO:0007270 : neuron-neuron synaptic transmission
- GO:0030432 : peristalsis
- GO:0046488 : phosphatidylinositol metabolic process
- GO:0060158 : phospholipase C-activating dopamine receptor signaling pathway
- GO:0043473 : pigmentation
- GO:0051586 : positive regulation of dopamine uptake involved in synaptic transmission
- GO:0070374 : positive regulation of ERK1 and ERK2 cascade
- GO:0045745 : positive regulation of G-protein coupled receptor protein signaling pathway
- GO:0060124 : positive regulation of growth hormone secretion
- GO:0040018 : positive regulation of multicellular organism growth
- GO:0002052 : positive regulation of neuroblast proliferation
- GO:0002092 : positive regulation of receptor internalization
- GO:0035815 : positive regulation of renal sodium excretion
- GO:0045944 : positive regulation of transcription from RNA polymerase II promoter
- GO:0035810 : positive regulation of urine volume
- GO:0060134 : prepulse inhibition
- GO:0008104 : protein localization
- GO:0051481 : reduction of cytosolic calcium ion concentration
- GO:0030814 : regulation of cAMP metabolic process
- GO:0014059 : regulation of dopamine secretion
- GO:0002027 : regulation of heart rate
- GO:0048169 : regulation of long-term neuronal synaptic plasticity
- GO:0043666 : regulation of phosphoprotein phosphatase activity
- GO:0043266 : regulation of potassium ion transport
- GO:0002028 : regulation of sodium ion transport
- GO:0032228 : regulation of synaptic transmission, GABAergic
- GO:0051209 : release of sequestered calcium ion into cytosol
- GO:0001975 : response to amphetamine
- GO:0048678 : response to axon injury
- GO:0042493 : response to drug
- GO:0034776 : response to histamine
- GO:0001666 : response to hypoxia
- GO:0010039 : response to iron ion
- GO:0043278 : response to morphine
- GO:0035094 : response to nicotine
- GO:0009636 : response to toxin
- GO:0007608 : sensory perception of smell
- GO:0007416 : synapse assembly
- GO:0001963 : synaptic transmission, dopaminergic
- GO:0001659 : temperature homeostasis
- GO:0008542 : visual learning
- GO:0016055 : Wnt receptor signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:126450 : Dystonia, myoclonic (OMIM:159900 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | D2R | Yeast two-hybrid | Not used as a bait |  | Human | Fetal brain | cDNA library |  | 18261457
TRPC4 |  | D2R | Inference |  |  |  |  |  | Prediction | 18261457
TRPC5 |  | D2R | Inference |  |  |  |  |  | Prediction | 18261457

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC1 |  | D2R | Fusion protein-pull down assay | Human | 638-759 | E. coli | Human | 131-151 | E. coli | 18261457
TRPC4 |  | D2R | Fusion protein-pull down assay | Human | 621-893 | E. coli | Human | 131-151 | E. coli | 18261457
TRPC5 |  | D2R | Fusion protein-pull down assay | Human | 619-973 | E. coli | Human | 131-151 | E. coli | 18261457

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | D2R | Co-immunofluorescence staining | Monkey prefrontal cortex |  |  |  |  |  | 18261457
TRPC1 |  | D2R | Co-immunoprecipitation | Rat striatal tissue |  |  |  |  |  | 18261457
TRPC1 |  | D2R | Co-immunoprecipitation | Rat cortex tissue |  |  |  |  |  | 18261457

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | D2R | Yeast two-hybrid | Human | 726-745 | Human | 131-151 |  |  | 18261457
TRPC4 |  | D2R | Yeast two-hybrid | Human | 712-731 | Human | 131-151 |  |  | 18261457
TRPC5 |  | D2R | Yeast two-hybrid | Human | 719-738 | Human | 131-151 |  |  | 18261457

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC1 |  | D2R | Cell surface biotinylation |  | Increase in plasma membrane level |  | 18261457

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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