# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPV5 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (17)
- Biological Process (1)
- Disease (0)

Name: Transient receptor potential cation channel subfamily V member 5 (TRPV5)
Official Symbol: TRPV5 provided by HGNC
Function:
Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 56302 | Q9NQA5 | DIP-43887N | Q9NQA5 | MINT-1470011 | 121137 | Q9NQA5 | 511 | hsa:56302 | 606679
Mouse | 194352 | P69744 | DIP-34874N | P69744 |  | 228781 | P69744 |  | mmu:194352 |
Rat | 116469 | Q9JIP0 |  |  | MINT-5159302 | 250447 | Q9JIP0 |  | rno:116469 |

PPI pairs:

80K-H | BSPRY | Calbindin-D28K | Calmodulin | Cyclophilin B | EWSR1 | FKBP52/FKBP4 | Galectin-1
Klotho | NHERF2 | NHERF4 | Nipsnap1 | Rab11a | S100A10 | TGM2 | TRPML3
TRPV6

Biological Process:

- GO:0051262 : protein tetramerization

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV5 |  | 80K-H | Inference |  |  |  |  |  | Prediction | 15100231
TRPV5 |  | BSPRY | Inference |  |  |  |  |  | Prediction | 16380433
TRPV5 |  | Calbindin-D28K | Inference |  |  |  |  |  | Prediction | 16763551
TRPV5 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 15123711
TRPV5 |  | Cyclophilin B | Inference |  |  |  |  |  | Prediction | 18445599
TRPV5 |  | EWSR1 | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified |  | 16189514
TRPV5 |  | FKBP52/FKBP4 | Inference |  |  |  |  |  | Prediction | 16352746
TRPV5 |  | Galectin-1 | Inference |  |  |  |  |  | Prediction | 18606998
TRPV5 |  | Klotho | Inference |  |  |  |  |  | Prediction | 16239475
TRPV5 |  | NHERF2 | Inference |  |  |  |  |  | Prediction | 15665527
TRPV5 |  | NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 16565876
TRPV5 |  | Nipsnap1 | Inference |  |  |  |  |  | Prediction | 18392847
TRPV5 |  | Rab11a | Inference |  |  |  |  |  | Prediction | 16354700
TRPV5 |  | S100A10 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 12660155
TRPV5 |  | TGM2 | Inference |  |  |  |  |  | Prediction | 21952826
TRPV5 |  | TRPML3 | Inference |  |  |  |  |  | Prediction | 23469151
TRPV5 |  | TRPV6 | Inference |  |  |  |  |  | Prediction | 12574114

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV5 |  | 80K-H | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 15100231
TRPV5 |  | BSPRY | Fusion protein-pull down assay | Mouse | 591-595 | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 16380433
TRPV5 |  | BSPRY | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 16380433
TRPV5 |  | Calbindin-D28K | Fusion protein-pull down assay | Mouse | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551
TRPV5 |  | Calbindin-D28K | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-327 | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 578-730 | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV5 |  | FKBP52/FKBP4 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 16352746
TRPV5 |  | NHERF2 | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 15665527
TRPV5 |  | NHERF2 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Not specified | Full-length | In vitro translation | 16565876
TRPV5 |  | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876
TRPV5 |  | Rab11a | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 16354700
TRPV5 |  | S100A10 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 12660155
TRPV5 |  | TGM2 | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Guinea pig | Not specified | Guinea pig liver | 21952826

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | 80K-H | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 15100231
TRPV5 |  | 80K-H | Co-immunoprecipitation |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 15100231
TRPV5 |  | BSPRY | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16380433
TRPV5 |  | BSPRY | Co-immunoprecipitation |  | MDCK | Mouse | Full-length | Mouse | Full-length | 16380433
TRPV5 |  | Calbindin-D28K | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16763551
TRPV5 |  | Calbindin-D28K | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not specified | Full-length | 16763551
TRPV5 |  | FKBP52/FKBP4 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16352746
TRPV5 |  | FKBP52/FKBP4 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 16352746
TRPV5 |  | Galectin-1 | Co-immunoprecipitation |  | HEK293 | Rabbit | Full-length | Not used |  | 18606998
TRPV5 |  | Klotho | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16239475
TRPV5 |  | Klotho | Co-immunoprecipitation |  | mIMCD-3 | Human | Full-length | Mouse | Full-length | 23747339
TRPV5 |  | NHERF4 | Co-immunofluorescence staining |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16565876
TRPV5 |  | NHERF4 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16565876
TRPV5 |  | NHERF4 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876
TRPV5 |  | Nipsnap1 | Co-immunoprecipitation |  | HEK293 | Rabbit | Full-length | Not specified | Full-length | 18392847
TRPV5 |  | Rab11a | Co-immunofluorescence staining |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700
TRPV5 |  | Rab11a | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16354700
TRPV5 |  | Rab11a | Co-immunoprecipitation |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700
TRPV5 |  | S100A10 | Co-immunofluorescence staining | Rat duodenum tissue |  |  |  |  |  | 12660155
TRPV5 |  | TRPML3 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Not specified | Full-length | 23469151
TRPV5 |  | TRPV6 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 12574114
TRPV5 |  | TRPV6 | Co-immunoprecipitation | Human myeloid leukemia K562 cell |  |  |  |  |  | 19295174
TRPV5 |  | TRPV6 | Co-immunoprecipitation |  | Xenopus oocyte | Rat | Full-length | Mouse | Full-length | 12574114
TRPV5 |  | TRPV6 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749
TRPV5 |  | TRPV6 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Not specified | Full-length | 23469151
TRPV5 |  | TRPV6 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | 80K-H | Fusion protein-pull down assay | Mouse | 598-608 | Mouse | Not determined |  |  | 15100231
TRPV5 |  | Calmodulin | Fluorescence probe labeling | Human | 587-616 | Not specified | Not determined |  | 306 nM | 20686800
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV5 |  | Calmodulin | Fusion protein-pull down assay | Human | 696-729 | Not specified | Not determined |  |  | 21576356
TRPV5 |  | Calmodulin | Native-gel electrophoresis | Human | 696-729 | Not specified | Not determined |  |  | 21576356
TRPV5 |  | NHERF2 | Fusion protein-pull down assay | Mouse | C-terminus | Mouse | Not determined |  |  | 15665527
TRPV5 |  | NHERF4 | Fusion protein-pull down assay | Mouse | 579-617 | Mouse | Not determined |  |  | 16565876
TRPV5 |  | Rab11a | Fusion protein-pull down assay | Mouse | 596-600 | Mouse | Not determined |  |  | 16354700
TRPV5 |  | S100A10 | Fusion protein-pull down assay | Mouse | 591-595 | Mouse | Not determined |  |  | 12660155
TRPV5 |  | TRPML3 | Patch clamp |  |  |  |  | 1:1 |  | 23469151

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV5 |  | BSPRY | Calcium measurement |  |  | Inhibition | 16380433
TRPV5 |  | Calbindin-D28K | Patch clamp |  |  | Activation | 16763551
TRPV5 |  | Calmodulin | Calcium measurement |  |  | Inhibition | 21576356
TRPV5 |  | Calmodulin | Patch clamp |  |  | Inhibition | 21576356
TRPV5 |  | Cyclophilin B | Calcium measurement |  |  | Activation | 18445599
TRPV5 |  | FKBP52/FKBP4 | Calcium measurement |  |  | Inhibition | 16352746
TRPV5 |  | FKBP52/FKBP4 | Patch clamp |  |  | Inhibition | 16352746
TRPV5 |  | Klotho | Calcium measurement |  |  | Activation | 16239475
TRPV5 |  | Klotho | Calcium measurement |  |  | Activation | 18495742
TRPV5 |  | Klotho | Cell surface biotinylation |  | Increase in plasma membrane level |  | 16239475
TRPV5 |  | Klotho | Cell surface biotinylation |  | Increase in plasma membrane level |  | 18606998
TRPV5 |  | Klotho | In vitro PTM assay | Glycosylation (Asn-358) |  |  | 16239475
TRPV5 |  | Klotho | Patch clamp |  |  | Activation | 16239475
TRPV5 |  | Rab11a | Calcium measurement |  |  | Activation | 16354700
TRPV5 |  | Rab11a | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 16354700
TRPV5 |  | S100A10 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 12660155
TRPV5 |  | S100A10 | Patch clamp |  |  | Activation | 12660155
TRPV5 |  | TGM2 | Calcium measurement |  |  | Inhibition | 21952826
TRPV5 |  | TGM2 | Cell surface biotinylation |  | Decrease in plasma membrane level |  | 21952826
TRPV5 |  | TGM2 | Patch clamp |  |  | Inhibition | 21952826
TRPV5 |  | TRPML3 | Patch clamp |  |  | New channel creation | 23469151
TRPV5 |  | TRPV6 | Patch clamp |  |  | New channel creation | 12574114

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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