# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# HSC70 / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (14)
- Disease (0)

Name: Heat shock cognate 71 kDa protein (HSC70)
Official Symbol: HSPA8 provided by HGNC
Function:
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 3312 | P11142 | DIP-32874N | P11142 | MINT-4998609 | 109544 | P11142 |  | hsa:3312 | 600816
Mouse | 15481 | P63017 | DIP-32353N | P63017 | MINT-189032 | 200428 | P63017 |  | mmu:15481 |
Rat | 24468 | P63018 | DIP-33262N | P63018 | MINT-2514077 | 246629 | P63018 |  | rno:24468 |

PPI pairs:

TRPML1 | TRPML2 | TRPML3

Biological Process:

- GO:0016044 : cellular membrane organization
- GO:0051085 : chaperone mediated protein folding requiring cofactor
- GO:0010467 : gene expression
- GO:0016071 : mRNA metabolic process
- GO:0006397 : mRNA processing
- GO:0045892 : negative regulation of transcription, DNA-dependent
- GO:0007269 : neurotransmitter secretion
- GO:0006892 : post-Golgi vesicle-mediated transport
- GO:0006457 : protein folding
- GO:0051726 : regulation of cell cycle
- GO:0006986 : response to unfolded protein
- GO:0008380 : RNA splicing
- GO:0006351 : transcription, DNA-dependent
- GO:0019048 : virus-host interaction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPML1 |  | HSC70 | Yeast two-hybrid | Human | 94-298 | Human | Brain | cDNA library |  | 19117012
TRPML2 |  | HSC70 | Inference |  |  |  |  |  | Prediction | 19117012
TRPML3 |  | HSC70 | Inference |  |  |  |  |  | Prediction | 19117012

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPML1 |  | HSC70 | Fusion protein-pull down assay | Human | 94-298 | In vitro translation | Human | Full-length | In vitro translation | 19117012

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPML1 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Human | Full-length | 19117012
TRPML1 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML2 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML3 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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