# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Junctate / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (18)
- Disease (0)

Name: Aspartyl/asparaginyl beta-hydroxylase (Junctate)
Official Symbol: ASPH provided by HGNC
Function:
Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.Isoform 8: membrane-bound Ca(2+)-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 444 | Q12797 |  | Q12797 | UNK-5841786 | 106936 | Q12797 |  | hsa:444 | 600582
Mouse | 65973 | Q8BSY0 |  |  | MINT-4112262 | 211167 | Q8BSY0 |  | mmu:65973 |
Rat | 312981 |  |  |  |  | 260287 |  |  |  |

PPI pairs:

TRPC2 | TRPC3 | TRPC5

Biological Process:

- GO:0032237 : activation of store-operated calcium channel activity
- GO:0070588 : calcium ion transmembrane transport
- GO:0071277 : cellular response to calcium ion
- GO:0060325 : face morphogenesis
- GO:0035108 : limb morphogenesis
- GO:0006936 : muscle contraction
- GO:0008285 : negative regulation of cell proliferation
- GO:0060315 : negative regulation of ryanodine-sensitive calcium-release channel activity
- GO:0060021 : palate development
- GO:0007389 : pattern specification process
- GO:0042264 : peptidyl-aspartic acid hydroxylation
- GO:0010524 : positive regulation of calcium ion transport into cytosol
- GO:0090316 : positive regulation of intracellular protein transport
- GO:0060316 : positive regulation of ryanodine-sensitive calcium-release channel activity
- GO:0045893 : positive regulation of transcription, DNA-dependent
- GO:0031585 : regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
- GO:0010880 : regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
- GO:0033198 : response to ATP

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC2 |  | Junctate | Inference |  |  |  |  |  | Prediction | 16153633
TRPC3 |  | Junctate | Inference |  |  |  |  |  | Prediction | 15302852
TRPC5 |  | Junctate | Inference |  |  |  |  |  | Prediction | 16153633

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC2 |  | Junctate | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Mouse | Full-length | HEK293 lysates | 16153633
TRPC3 |  | Junctate | Fusion protein-pull down assay | Bovine | Full-length | HEK293 lysates | Not specified | 1-85 | Not specified | 15302852
TRPC5 |  | Junctate | Fusion protein-pull down assay | Not specified | Full-length | HEK293 lysates | Mouse | Full-length | In vitro translation | 16153633

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC2 |  | Junctate | Fusion protein-pull down assay | Mouse | 925-1172 | Mouse | 1-25 (in isoform Junctin) |  |  | 16153633

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC3 |  | Junctate | Patch clamp |  |  | Activation | 15302852

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.