# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# REA / TRP channels

- Basic Information
- External Database
- PPI pairs (1)
- Biological Process (7)
- Disease (0)

Name: Prohibitin-2 (REA)
Official Symbol: PHB2 provided by HGNC
Function:
Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 11331 | Q99623 | DIP-31151N | Q99623 | MINT-142400 | 116459 | Q99623 |  | hsa:11331 | 610704
Mouse | 12034 | O35129 | DIP-31925N | O35129 | MINT-1841450 | 198310 | O35129 |  | mmu:12034 |
Rat | 114766 | Q5XIH7 | DIP-37276N | Q5XIH7 | MINT-4594704 | 250411 | Q5XIH7 |  | rno:114766 |

PPI pairs:

TRPM6

Biological Process:

- GO:0060749 : mammary gland alveolus development
- GO:0060744 : mammary gland branching involved in thelarche
- GO:0033147 : negative regulation of intracellular estrogen receptor signaling pathway
- GO:0033600 : negative regulation of mammary gland epithelial cell proliferation
- GO:0045892 : negative regulation of transcription, DNA-dependent
- GO:0060762 : regulation of branching involved in mammary gland duct morphogenesis
- GO:0006351 : transcription, DNA-dependent

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM6 |  | REA | Affinity purification-mass spectrometry | Mouse | Full-length | Mouse | Kidney | Tissue lysates |  | 19329436

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM6 |  | REA | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 19329436

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM6 |  | REA | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Mouse | Full-length | 19329436

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM6 |  | REA | Fusion protein-pull down assay | Mouse | 1857-1885 | Mouse | Not determined |  |  | 19329436

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM6 |  | REA | Patch clamp |  |  | Inhibition | 19329436

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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