# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# I-MFA / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (9)
- Disease (0)

Name: MyoD family inhibitor (I-MFA)
Official Symbol: MDFI provided by HGNC
Function:
Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 4188 | Q99750 | DIP-34420N | Q99750 | MINT-190174 | 110353 | Q99750 |  | hsa:4188 | 604971
Mouse | 17240 | P70331 | DIP-41709N |  | MINT-1519234 | 201368 | P70331 |  | mmu:17240 |
Rat | 501097 | B0BN88 |  |  |  | 272404 | B0BN88 |  | rno:501097 |

PPI pairs:

TRPC1 | TRPV6

Biological Process:

- GO:0007257 : activation of JUN kinase activity
- GO:0042994 : cytoplasmic sequestering of transcription factor
- GO:0009950 : dorsal/ventral axis specification
- GO:0009790 : embryo development
- GO:0048704 : embryonic skeletal system morphogenesis
- GO:0043392 : negative regulation of DNA binding
- GO:0000122 : negative regulation of transcription from RNA polymerase II promoter
- GO:0030178 : negative regulation of Wnt receptor signaling pathway
- GO:0060707 : trophoblast giant cell differentiation

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | I-MFA | Yeast two-hybrid | Human | 639-750 | Mouse | Embryonic | cDNA library |  | 14530267
TRPC1 |  | I-MFA | Yeast two-hybrid | Human | 639-750 | Human | Fetal kidney | cDNA library |  | 14530267
TRPV6 |  | I-MFA | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified |  | 16189514

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC1 |  | I-MFA | Fusion protein-pull down assay | Human | 639-750 | E. coli | Human | Full-length | E. coli | 14530267

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | I-MFA | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 14530267
TRPC1 |  | I-MFA | Co-immunoprecipitation | Rat liver tissue |  |  |  |  |  | 14530267
TRPC1 |  | I-MFA | Co-immunoprecipitation | NALM-6 |  |  |  |  |  | 14530267
TRPC1 |  | I-MFA | Co-immunoprecipitation | Rat kidney tissue |  |  |  |  |  | 14530267
TRPC1 |  | I-MFA | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 23770672

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC1 |  | I-MFA | Patch clamp |  |  | Inhibition | 14530267
TRPC1 |  | I-MFA | Patch clamp |  |  | Desensitization | 23770672

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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