# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPML3 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (8)
- Biological Process (2)
- Disease (0)

Name: Mucolipin-3 (TRPML3)
Official Symbol: MCOLN3 provided by HGNC
Function:
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 55283 | Q8TDD5 |  |  |  | 120571 | Q8TDD5 | 503 | hsa:55283 | 607400
Mouse | 171166 | Q8R4F0 |  |  |  | 228482 | Q8R4F0 |  | mmu:171166 |
Rat | 308022 | Q6AY44 | DIP-39439N | Q6AY44 |  | 258885 | Q6AY44 |  | rno:308022 |

PPI pairs:

GATE16 | HSC70 | TPC1 | TPC2 | TRPML1 | TRPML2 | TRPV5 | TRPV6

Biological Process:

- GO:0042491 : auditory receptor cell differentiation
- GO:0007626 : locomotory behavior

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPML3 |  | GATE16 | Yeast two-hybrid | Human | 1-312 | Mouse | Brain | cDNA library |  | 24269818
TRPML3 |  | HSC70 | Inference |  |  |  |  |  | Prediction | 19117012
TRPML3 |  | TPC1 | Inference |  |  |  |  |  | Prediction | 21540176
TRPML3 |  | TPC2 | Inference |  |  |  |  |  | Prediction | 21540176
TRPML3 |  | TRPML1 | Inference |  |  |  |  |  | Prediction | 16606612
TRPML3 |  | TRPML2 | Inference |  |  |  |  |  | Prediction | 16606612
TRPML3 |  | TRPV5 | Inference |  |  |  |  |  | Prediction | 23469151
TRPML3 |  | TRPV6 | Inference |  |  |  |  |  | Prediction | 23469151

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPML3 |  | GATE16 | Fusion protein-pull down assay | Human | Full-length | HEK293T lysates | Mouse | Full-length | E. coli | 24269818

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPML3 |  | GATE16 | Co-immunofluorescence staining |  | HeLa | Human | Full-length | Mouse | Full-length | 24269818
TRPML3 |  | GATE16 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Mouse | Full-length | 24269818
TRPML3 |  | HSC70 | Co-immunoprecipitation |  | CHO | Human | Full-length | Not used |  | 19117012
TRPML3 |  | TPC1 | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC1 | Co-immunofluorescence staining |  | HeLa | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC1 | Co-immunofluorescence staining |  | SK-BR-3 | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC1 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC2 | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC2 | Co-immunofluorescence staining |  | HeLa | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC2 | Co-immunofluorescence staining |  | SK-BR-3 | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TPC2 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 21540176
TRPML3 |  | TRPML1 | Co-immunofluorescence staining | HEK293 |  |  |  |  |  | 19557826
TRPML3 |  | TRPML1 | Co-immunofluorescence staining |  | CHO | Human | Full-length | Human | Full-length | 19885840
TRPML3 |  | TRPML1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not specified | Full-length | 19522758
TRPML3 |  | TRPML1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 19557826
TRPML3 |  | TRPML1 | Co-immunoprecipitation |  | CHO | Human | Full-length | Human | Full-length | 19885840
TRPML3 |  | TRPML1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length (mutation in the pore region) | Human | Full-length (mutation in the pore region) | 20736310
TRPML3 |  | TRPML1 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151
TRPML3 |  | TRPML1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 16606612
TRPML3 |  | TRPML2 | Co-immunofluorescence staining | HEK293 |  |  |  |  |  | 19557826
TRPML3 |  | TRPML2 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 19557826
TRPML3 |  | TRPML2 | Co-immunoprecipitation |  | HEK293 | Human | Full-length (mutation in the pore region) | Human | Full-length (mutation in the pore region) | 20736310
TRPML3 |  | TRPML2 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151
TRPML3 |  | TRPML2 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 16606612
TRPML3 |  | TRPV5 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Mouse | Full-length | 23469151
TRPML3 |  | TRPV6 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPML3 |  | GATE16 | Co-immunoprecipitation | Human | 1-312 | Mouse | Not determined |  |  | 24269818
TRPML3 |  | GATE16 | Fusion protein-pull down assay | Human | 1-312 | Mouse | Not determined |  |  | 24269818
TRPML3 |  | TRPV5 | Patch clamp |  |  |  |  | 1:1 |  | 23469151

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPML3 |  | TRPML1 | Co-immunofluorescence staining |  | Increase in lysosomal membrane level |  | 16606612
TRPML3 |  | TRPML1 | Patch clamp |  |  | New channel creation | 19885840
TRPML3 |  | TRPML2 | Co-immunofluorescence staining |  | Increase in lysosomal membrane level |  | 16606612
TRPML3 |  | TRPV5 | Patch clamp |  |  | New channel creation | 23469151

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.