# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPC7 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (12)
- Biological Process (3)
- Disease (0)

Name: Short transient receptor potential channel 7 (TRPC7)
Official Symbol: TRPC7 provided by HGNC
Function:
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 57113 | Q9HCX4 | DIP-35697N | Q9HCX4 |  | 121378 | Q9HCX4 | 492 | hsa:57113 |
Mouse | 26946 | Q9WVC5 |  |  |  | 205082 | Q9WVC5 |  | mmu:26946 |
Rat | 282822 | Q6TPJ4 |  |  |  | 251859 | Q6TPJ4 |  | rno:282822 |

PPI pairs:

Calmodulin | Cav1.2 | FKBP12 | IP3R3 | MxA | PKG1 | RNF24 | TRPC1
TRPC3 | TRPC5 | TRPC6 | TRPP1

Biological Process:

- GO:0007411 : axon guidance
- GO:0006828 : manganese ion transport
- GO:0030168 : platelet activation

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC7 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC7 |  | Cav1.2 | Inference |  |  |  |  |  | Prediction | 21672930
TRPC7 |  | FKBP12 | Inference |  |  |  |  |  | Prediction | 15199065
TRPC7 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC7 |  | MxA | Inference |  |  |  |  |  | Prediction | 15757897
TRPC7 |  | PKG1 | Inference |  |  |  |  |  | Prediction | 21402151
TRPC7 |  | RNF24 | Inference |  |  |  |  |  | Prediction | 17850865
TRPC7 |  | TRPC1 | Inference |  |  |  |  |  | Prediction | 15972814
TRPC7 |  | TRPC3 | Inference |  |  |  |  |  | Prediction | 12032305
TRPC7 |  | TRPC5 | Inference |  |  |  |  |  | Prediction | 18356201
TRPC7 |  | TRPC6 | Inference |  |  |  |  |  | Prediction | 12032305
TRPC7 |  | TRPP1 | Inference |  |  |  |  |  | Prediction | 19812035

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC7 |  | FKBP12 | Co-immunoprecipitation | Mouse | Full-length | Sf9 lysates | Human | Full-length | Sf9 lysates | 15199065
TRPC7 |  | MxA | Fusion protein-pull down assay | Human | 3-195 | In vitro translation | Human | 572-662 | E. coli | 15757897
TRPC7 |  | RNF24 | Fusion protein-pull down assay | Not specified | 3-195 | In vitro translation | Human | 98-148 | E. coli | 17850865

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC7 |  | Cav1.2 | Co-immunoprecipitation | Chick embryonic heart lysates |  |  |  |  |  | 21672930
TRPC7 |  | FKBP12 | Co-immunoprecipitation | Rat cerebral cortex |  |  |  |  |  | 15199065
TRPC7 |  | PKG1 | Co-immunoprecipitation |  | COS-7 | Mouse | Full-length | Human | Full-length | 21402151
TRPC7 |  | TRPC1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 15972814
TRPC7 |  | TRPC3 | Co-immunoprecipitation |  | Sf9 | Mouse | Full-length | Human | Full-length | 12377790
TRPC7 |  | TRPC3 | Co-immunoprecipitation | Rat brain synaptome |  |  |  |  |  | 12377790
TRPC7 |  | TRPC3 | Co-immunoprecipitation |  | CHO | Mouse | 1-494 | Human | Full-length | 15537648
TRPC7 |  | TRPC3 | Co-immunoprecipitation | Rat ventricular myocytes |  |  |  |  |  | 18502908
TRPC7 |  | TRPC3 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 12032305
TRPC7 |  | TRPC5 | Co-immunoprecipitation | Rabbit portal vein |  |  |  |  |  | 18356201
TRPC7 |  | TRPC6 | Co-immunoprecipitation |  | Sf9 | Mouse | Full-length | Mouse | Full-length | 12377790
TRPC7 |  | TRPC6 | Co-immunoprecipitation | Rat brain synaptome |  |  |  |  |  | 12377790
TRPC7 |  | TRPC6 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 12032305
TRPC7 |  | TRPP1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | 1-697 | 19812035

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC7 |  | Calmodulin | Fluorescence probe labeling | Mouse | 769-798 | Not specified | Not determined |  | 19 nM | 11290752
TRPC7 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 766-798 | Not specified | Not determined |  |  | 11290752
TRPC7 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 766-798 | Human | 669-698 |  |  | 11290752
TRPC7 |  | MxA | Fusion protein-pull down assay | Human | 3-195 | Human | 572-662 |  |  | 15757897
TRPC7 |  | PKG1 | Co-immunoprecipitation | Mouse | 35-200 | Human | 105-360 |  |  | 21402151
TRPC7 |  | PKG1 | Co-immunoprecipitation | Mouse | 35-200 | Human | 1-360 |  |  | 21402151

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC7 |  | PKG1 | In vitro PTM assay | Phosphorylation |  |  | 21402151
TRPC7 |  | TRPP1 | Calcium measurement |  |  | Activation | 19812035

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.