# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Homer-1 / TRP channels

- Basic Information
- External Database
- PPI pairs (4)
- Biological Process (8)
- Disease (0)

Name: Homer protein homolog 1 (Homer-1)
Official Symbol: HOMER1 provided by HGNC
Function:
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 9456 | Q86YM7 | DIP-42866N | Q86YM7 | MINT-1481241 | 114845 | Q86YM7 |  | hsa:9456 | 604798
Mouse | 26556 | Q9Z2Y3 | DIP-31580N | Q9Z2Y3 | MINT-3370083 | 205014 | Q9Z2Y3 |  | mmu:26556 |
Rat | 29546 | Q9Z214 | DIP-44898N | Q9Z214 | MINT-1891037 | 248180 | Q9Z214 |  | rno:29546 |

PPI pairs:

TRPC1 | TRPC2 | TRPC3 | TRPC5

Biological Process:

- GO:0007206 : phospholipase C-activating G-protein coupled glutamate receptor signaling pathway
- GO:0090279 : regulation of calcium ion import
- GO:2001257 : regulation of cation channel activity
- GO:2001256 : regulation of store-operated calcium entry
- GO:0051592 : response to calcium ion
- GO:0003009 : skeletal muscle contraction
- GO:0048741 : skeletal muscle fiber development
- GO:0007268 : synaptic transmission

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | Homer-1 | Inference |  |  |  |  |  | Prediction | 14505576
TRPC2 |  | Homer-1 | Inference |  |  |  |  |  | Prediction | 14505576
TRPC3 |  | Homer-1 | Inference |  |  |  |  |  | Prediction | 16887806
TRPC5 |  | Homer-1 | Inference |  |  |  |  |  | Prediction | 14505576

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC3 |  | Homer-1 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | Full-length | E. coli | 16887806

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | Homer-1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 14505576
TRPC1 |  | Homer-1 | Co-immunoprecipitation | Rat brain hippocampus |  |  |  |  |  | 14505576
TRPC1 |  | Homer-1 | Co-immunoprecipitation | Rat astrocyte |  |  |  |  |  | 16905188
TRPC1 |  | Homer-1 | Co-immunoprecipitation | Human platelets |  |  |  |  |  | 22506990
TRPC2 |  | Homer-1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC2 |  | Homer-1 | Co-immunoprecipitation | Rat vomeronasal organ lysates |  |  |  |  |  | 20492691
TRPC5 |  | Homer-1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC3 |  | Homer-1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 16887806
TRPC3 |  | Homer-1 | Patch clamp |  |  | Activation | 16887806

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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