# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# EGFR / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (31)
- Disease (3)

Name: Epidermal growth factor receptor (EGFR)
Official Symbol: EGFR provided by HGNC
Function:
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo-and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 1956 | P00533 | DIP-405N DIP-5764N | P00533 | MINT-206389 | 108276 | P00533 |  | hsa:1956 | 131550
Mouse | 13649 | Q01279 | DIP-5763N |  | MINT-143143 | 199402 | Q01279 |  | mmu:13649 |
Rat | 24329 | Q9QX70 | DIP-2658N | Q9QX70 | MINT-1509350 | 246506 | Q9QX70 |  | rno:24329 |

PPI pairs:

TRPP1 | TRPV1 | TRPV3

Biological Process:

- GO:0043006 : activation of phospholipase A2 activity by calcium-mediated signaling
- GO:0007202 : activation of phospholipase C activity
- GO:0007411 : axon guidance
- GO:0008283 : cell proliferation
- GO:0016337 : cell-cell adhesion
- GO:0021795 : cerebral cortex cell migration
- GO:0048546 : digestive tract morphogenesis
- GO:0001892 : embryonic placenta development
- GO:0001942 : hair follicle development
- GO:0060571 : morphogenesis of an epithelial fold
- GO:0043066 : negative regulation of apoptotic process
- GO:0042059 : negative regulation of epidermal growth factor receptor signaling pathway
- GO:0042177 : negative regulation of protein catabolic process
- GO:0001503 : ossification
- GO:0035413 : positive regulation of catenin import into nucleus
- GO:0030335 : positive regulation of cell migration
- GO:0031659 : positive regulation of cyclin-dependent protein kinase activity involved in G1/S
- GO:0045739 : positive regulation of DNA repair
- GO:0045740 : positive regulation of DNA replication
- GO:0050679 : positive regulation of epithelial cell proliferation
- GO:0048146 : positive regulation of fibroblast proliferation
- GO:0043406 : positive regulation of MAP kinase activity
- GO:0045429 : positive regulation of nitric oxide biosynthetic process
- GO:0051897 : positive regulation of protein kinase B signaling cascade
- GO:0046777 : protein autophosphorylation
- GO:0051205 : protein insertion into membrane
- GO:0050999 : regulation of nitric-oxide synthase activity
- GO:0050730 : regulation of peptidyl-tyrosine phosphorylation
- GO:0006950 : response to stress
- GO:0070141 : response to UV-A
- GO:0007435 : salivary gland morphogenesis

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:131550 : Nonsmall cell lung cancer, response to tyrosine kinase inhibitor in (OMIM:211980 )
- OMIM:131550 : Adenocarcinoma of lung, response to tyrosine kinase inhibitor in (OMIM:211980 )
- OMIM:131550 : Nonsmall cell lung cancer, susceptibility to (OMIM:211980 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPP1 |  | EGFR | Inference |  |  |  |  |  | Prediction | 16135816
TRPV1 |  | EGFR | Inference |  |  |  |  |  | Prediction | 19155296
TRPV3 |  | EGFR | Inference |  |  |  |  |  | Prediction | 20403327

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV1 |  | EGFR | Fusion protein-pull down assay | Not specified | 680-838 | HEK293 lysates | Not used |  | A431 Human epithelial carcinoma cell line | 19155296

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | EGFR | Co-immunofluorescence staining | LLC-PK1 |  |  |  |  |  | 16135816
TRPP1 |  | EGFR | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 16135816
TRPV1 |  | EGFR | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Not used |  | 19155296
TRPV1 |  | EGFR | Co-immunoprecipitation | HaCaT |  |  |  |  |  | 19155296
TRPV3 |  | EGFR | Co-immunoprecipitation | TRPV3-YFP transgenic mouse skin tissue |  |  |  |  |  | 20403327

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | EGFR | Co-immunoprecipitation | Human | 1-871 | Human | Not determined |  |  | 16135816

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV3 |  | EGFR | Calcium measurement |  |  | Activation | 20403327

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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