# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# IP3R3 / TRP channels

- Basic Information
- External Database
- PPI pairs (9)
- Biological Process (16)
- Disease (0)

Name: Inositol 1,4,5-trisphosphate receptor type 3 (IP3R3)
Official Symbol: ITPR3 provided by HGNC
Function:
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 3710 | Q14573 | DIP-33130N | Q14573 | MINT-4991384 | 109915 | Q14573 |  | hsa:3710 | 147267
Mouse | 16440 | P70227 |  |  | UNK-5382532 | 200849 | P70227 |  | mmu:16440 |
Rat | 25679 | Q63269 |  |  | UNK-5464541 | 247708 | Q63269 |  | rno:25679 |

PPI pairs:

TRPC1 | TRPC2 | TRPC3 | TRPC4 | TRPC5 | TRPC6 | TRPC7 | TRPP1
TRPV4

Biological Process:

- GO:0007202 : activation of phospholipase C activity
- GO:0060402 : calcium ion transport into cytosol
- GO:0006112 : energy reserve metabolic process
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0007186 : G-protein coupled receptor signaling pathway
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0051291 : protein heterooligomerization
- GO:0051260 : protein homooligomerization
- GO:0050796 : regulation of insulin secretion
- GO:0051592 : response to calcium ion
- GO:0050913 : sensory perception of bitter taste
- GO:0050916 : sensory perception of sweet taste
- GO:0050917 : sensory perception of umami taste
- GO:0044281 : small molecule metabolic process

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 10766822
TRPC2 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC3 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 10611319
TRPC4 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC5 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC6 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 10611319
TRPC7 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPP1 |  | IP3R3 | Affinity purification-mass spectrometry | Mouse | Not specified | Mouse | Vascular smooth muscle | MOVAS lysates |  | 19879844
TRPV4 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 18378772

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC3 |  | IP3R3 | Fusion protein-pull down assay | Human | 676-848 | In vitro translation | Human | 638-926 | E. coli | 10611319
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Human | 669-698 | E. coli | 11290752
TRPC4 |  | IP3R3 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2556-2713 | Yeast | 11163362

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | IP3R3 | Co-immunofluorescence staining | Human keratinocyte |  |  |  |  |  | 15654973
TRPC1 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 14505576
TRPC1 |  | IP3R3 | Co-immunoprecipitation |  | HSG cell | Human | Full-length | Not used |  | 10766822
TRPC1 |  | IP3R3 | Co-immunoprecipitation |  | HSG cell | Human | Full-length | Not used |  | 10980191
TRPC1 |  | IP3R3 | Co-immunoprecipitation | Human keratinocyte |  |  |  |  |  | 15654973
TRPC2 |  | IP3R3 | Co-immunofluorescence staining | Rat vomeronasal organ |  |  |  |  |  | 12472899
TRPC2 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC2 |  | IP3R3 | Co-immunoprecipitation | Rat vomeronasal organ |  |  |  |  |  | 12472899
TRPC3 |  | IP3R3 | Co-immunofluorescence staining |  | HEK293 | Human | Full-length | Not used |  | 11524429
TRPC3 |  | IP3R3 | Co-immunofluorescence staining | Mouse pancreatic acini |  |  |  |  |  | 16887806
TRPC3 |  | IP3R3 | Co-immunofluorescence staining | Mouse parotid acini |  |  |  |  |  | 16887806
TRPC3 |  | IP3R3 | Co-immunofluorescence staining | Mouse submandibular gland duct |  |  |  |  |  | 16887806
TRPC3 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 14505576
TRPC3 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 11524429
TRPC3 |  | IP3R3 | Co-immunoprecipitation | Mouse brain tissue |  |  |  |  |  | 16887806
TRPC3 |  | IP3R3 | Co-immunoprecipitation | Mouse pancreatic tissue |  |  |  |  |  | 16887806
TRPC3 |  | IP3R3 | Co-immunoprecipitation | Mouse submandibular gland lysates |  |  |  |  |  | 16887806
TRPC3 |  | IP3R3 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 10611319
TRPC3 |  | IP3R3 | Co-immunoprecipitation |  | MDCK | Not specified | Full-length | Not used |  | 15623527
TRPC3 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 10983988
TRPC4 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC5 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC6 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 14505576
TRPC6 |  | IP3R3 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Human | Full-length | 10611319
TRPP1 |  | IP3R3 | Co-immunofluorescence staining |  | LLC-PK1 | Human | Full-length | Not used |  | 20375013
TRPP1 |  | IP3R3 | Co-immunoprecipitation |  | LLC-PK1 | Human | Full-length | Not used |  | 20375013
TRPV4 |  | IP3R3 | Co-immunoprecipitation |  | HeLa | Human | Full-length | Rat | Full-length | 18378772
TRPV4 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Rat | Full-length | 18826956

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | IP3R3 | Co-immunoprecipitation | Human | 645-648 | Human | Not determined |  |  | 14505576
TRPC1 |  | IP3R3 | Co-immunoprecipitation | Human | 781-789 | Human | Not determined |  |  | 19052258
TRPC1 |  | IP3R3 | Fusion protein-pull down assay | Human | 719-751 | Human | 669-698 |  |  | 11290752
TRPC2 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 901-936 | Human | 669-698 |  |  | 11290752
TRPC3 |  | IP3R3 | Co-immunoprecipitation | Human | 775-787 | Rat | Not determined |  |  | 18276585
TRPC3 |  | IP3R3 | Fusion protein-pull down assay | Human | 777-797 | Human | 669-698 |  |  | 10611319
TRPC3 |  | IP3R3 | Fusion protein-pull down assay | Human | 777-797 | Human | 751-821 |  |  | 10611319
TRPC3 |  | IP3R3 | Fusion protein-pull down assay | Human | 761-795 | Human | 669-698 |  |  | 11248050
TRPC3 |  | IP3R3 | Fusion protein-pull down assay | Human | 761-795 | Human | 669-698 |  |  | 11290752
TRPC3 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 725-848 | Human | 681-698 |  |  | 11290752
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 |  |  | 15104175
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 781-864 | Human | 669-698 |  |  | 11290752
TRPC4 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 |  |  | 11290752
TRPC5 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 701-733 | Human | 669-698 |  |  | 11290752
TRPC6 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 838-872 | Human | 669-698 |  |  | 11290752
TRPC7 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 766-798 | Human | 669-698 |  |  | 11290752
TRPV4 |  | IP3R3 | Co-immunoprecipitation | Human | 812-831 | Rat | Not determined |  |  | 18826956

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC3 |  | IP3R3 | Calcium measurement |  |  | Activation | 10611319
TRPC3 |  | IP3R3 | Calcium measurement |  |  | Inhibition | 10611319
TRPC3 |  | IP3R3 | Patch clamp |  |  | Activation | 11248050
TRPV4 |  | IP3R3 | Calcium measurement |  |  | Activation | 18378772
TRPV4 |  | IP3R3 | Calcium measurement |  |  | Activation | 18826956
TRPV4 |  | IP3R3 | Patch clamp |  |  | Activation | 18378772
TRPV4 |  | IP3R3 | Patch clamp |  |  | Activation | 18826956

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.