# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPC2 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (13)
- Biological Process (8)
- Disease (0)

Name: Short transient receptor potential channel 2 (TRPC2)
Official Symbol: Trpc2 provided by HGNC
Function:
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. May also be activated by intracellular calcium store depletion.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human |  |  |  |  |  |  |  |  |  |
Mouse | 22064 | Q9R244 |  |  |  | 204328 | Q9R244 | 487 | mmu:22064 |
Rat | 64573 | Q9R283 |  |  |  | 249153 | Q9R283 |  | rno:64573 |

PPI pairs:

Calmodulin | Caspase-4/Caspase-11 | Enkurin | Epo-R | Homer-1 | IP3R2 | IP3R3 | Junctate
PLCƣ1 | RTP1 | STIM1 | Src | TRPC6

Biological Process:

- GO:0007340 : acrosome reaction
- GO:0007204 : elevation of cytosolic calcium ion concentration
- GO:0008050 : female courtship behavior
- GO:0002121 : inter-male aggressive behavior
- GO:0048047 : mating behavior, sex discrimination
- GO:0019236 : response to pheromone
- GO:0000012 : single strand break repair
- GO:0002124 : territorial aggressive behavior

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC2 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11290752
TRPC2 |  | Caspase-4/Caspase-11 | Speculation |  |  |  |  |  | Prediction | 24630989
TRPC2 |  | Enkurin | Yeast two-hybrid | Mouse | 34-526 | Mouse | Testis | cDNA library |  | 15385169
TRPC2 |  | Epo-R | Inference |  |  |  |  |  | Prediction | 12167663
TRPC2 |  | Epo-R | Inference |  |  |  |  |  | Prediction | 15329338
TRPC2 |  | Homer-1 | Inference |  |  |  |  |  | Prediction | 14505576
TRPC2 |  | IP3R2 | Inference |  |  |  |  |  | Prediction | 15329338
TRPC2 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 11290752
TRPC2 |  | Junctate | Inference |  |  |  |  |  | Prediction | 16153633
TRPC2 |  | PLCƣ1 | Inference |  |  |  |  |  | Prediction | 15329338
TRPC2 |  | RTP1 | Inference |  |  |  |  |  | Prediction | 20492691
TRPC2 |  | SRC | Inference |  |  |  |  |  | Prediction | 16407161
TRPC2 |  | STIM1 | Inference |  |  |  |  |  | Prediction | 16906149
TRPC2 |  | TRPC6 | Inference |  |  |  |  |  | Prediction | 14699131

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Not specified | Full-length | In vitro translation | 16153633
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 1-619 | In vitro translation | Not specified | Full-length | E. coli | 12601176
TRPC2 |  | Junctate | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Mouse | Full-length | HEK293 lysates | 16153633
TRPC2 |  | SRC | Fusion protein-pull down assay | Mouse | 1-616 | In vitro translation | Chicken | Full-length | E. coli | 16407161

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC2 |  | Caspase-4/Caspase-11 | Co-immunoprecipitation |  | HEK293T | Not specified | Full-length | Not specified | Full-length | 24630989
TRPC2 |  | Epo-R | Co-immunofluorescence staining |  | CHO | Mouse | Full-length | Mouse | Full-length | 12167663
TRPC2 |  | Epo-R | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Mouse | Full-length | 15329338
TRPC2 |  | Epo-R | Co-immunoprecipitation | Mouse erythroblasts |  |  |  |  |  | 15329338
TRPC2 |  | Epo-R | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Mouse | Full-length | 22088247
TRPC2 |  | Homer-1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC2 |  | Homer-1 | Co-immunoprecipitation | Rat vomeronasal organ lysates |  |  |  |  |  | 20492691
TRPC2 |  | IP3R2 | Co-immunofluorescence staining | Mouse splenic erythroblast |  |  |  |  |  | 15329338
TRPC2 |  | IP3R2 | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Rat | Full-length | 15329338
TRPC2 |  | IP3R2 | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Rat | Full-length | 22088247
TRPC2 |  | IP3R3 | Co-immunofluorescence staining | Rat vomeronasal organ |  |  |  |  |  | 12472899
TRPC2 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 14505576
TRPC2 |  | IP3R3 | Co-immunoprecipitation | Rat vomeronasal organ |  |  |  |  |  | 12472899
TRPC2 |  | PLCƣ1 | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Rat | Full-length | 15329338
TRPC2 |  | PLCƣ1 | Co-immunoprecipitation | Mouse erythroblasts |  |  |  |  |  | 15329338
TRPC2 |  | PLCƣ1 | Co-immunoprecipitation |  | CHO-S | Mouse | Full-length | Rat | Full-length | 22088247
TRPC2 |  | RTP1 | Co-immunofluorescence staining | Rat vomeronasal organ section |  |  |  |  |  | 20492691
TRPC2 |  | RTP1 | Co-immunoprecipitation | Rat vomeronasal organ lysates |  |  |  |  |  | 20492691
TRPC2 |  | STIM1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 16906149
TRPC2 |  | TRPC6 | Co-immunoprecipitation |  | CHO | Mouse | Full-length | Mouse | Full-length | 14699131
TRPC2 |  | TRPC6 | Co-immunoprecipitation | Mouse brain erythroblast |  |  |  |  |  | 14699131

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC2 |  | Calmodulin | Fluorescence probe labeling | Mouse | 905-934 | Not specified | Not determined |  | 10 nM | 11290752
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 104-195 | Not specified | Not determined |  |  | 12601176
TRPC2 |  | Calmodulin | Fusion protein-pull down assay | Mouse | 901-936 | Not specified | Not determined |  |  | 11290752
TRPC2 |  | IP3R2 | Co-immunoprecipitation | Mouse | 1001-1036 | Rat | Not determined |  |  | 15329338
TRPC2 |  | IP3R3 | Fusion protein-pull down assay | Mouse | 901-936 | Human | 669-698 |  |  | 11290752
TRPC2 |  | Junctate | Fusion protein-pull down assay | Mouse | 925-1172 | Mouse | 1-25 (in isoform Junctin) |  |  | 16153633
TRPC2 |  | STIM1 | Co-immunoprecipitation | Mouse | Not determined | Human | 251-535 |  |  | 16906149

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC2 |  | Epo-R | Calcium measurement |  |  | Activation | 12167663
TRPC2 |  | RTP1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 20492691

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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