# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# NCX1 / TRP channels

- Basic Information
- External Database
- PPI pairs (1)
- Biological Process (24)
- Disease (0)

Name: Sodium/calcium exchanger 1 (NCX1)
Official Symbol: SLC8A1 provided by HGNC
Function:
Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 6546 | P32418 | DIP-48356N | P32418 | MINT-4824013 | 112436 | P32418 |  | hsa:6546 | 182305
Mouse | 20541 | P70414 | DIP-54905N | P70414 |  | 203320 | P70414 |  | mmu:20541 |
Rat | 29715 | Q01728 |  |  | MINT-4563844 | 248332 | Q01728 |  | rno:29715 |

PPI pairs:

TRPC3

Biological Process:

- GO:0007596 : blood coagulation
- GO:0070509 : calcium ion import
- GO:0060402 : calcium ion transport into cytosol
- GO:0055013 : cardiac muscle cell development
- GO:0086064 : cell communication by electrical coupling involved in cardiac conduction
- GO:0071313 : cellular response to caffeine
- GO:0071320 : cellular response to cAMP
- GO:0034614 : cellular response to reactive oxygen species
- GO:0051481 : reduction of cytosolic calcium ion concentration
- GO:0010881 : regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
- GO:0002027 : regulation of heart rate
- GO:0002028 : regulation of sodium ion transport
- GO:0002026 : regulation of the force of heart contraction
- GO:0055119 : relaxation of cardiac muscle
- GO:0044557 : relaxation of smooth muscle
- GO:0033198 : response to ATP
- GO:0042493 : response to drug
- GO:0009749 : response to glucose stimulus
- GO:0042542 : response to hydrogen peroxide
- GO:0001666 : response to hypoxia
- GO:0007584 : response to nutrient
- GO:0071436 : sodium ion export
- GO:0021537 : telencephalon development
- GO:0014829 : vascular smooth muscle contraction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC3 |  | NCX1 | Inference |  |  |  |  |  | Prediction | 14736881

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC3 |  | NCX1 | Fusion protein-pull down assay | Human | 742-848 | E. coli | Not used |  | HEK293 lysates | 14736881
TRPC3 |  | NCX1 | Fusion protein-pull down assay | Human | 670-848 | E. coli | Not used |  | Rat Cardiomyocyte lysates | 17129578

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | NCX1 | Co-immunofluorescence staining | Rat myocyte section |  |  |  |  |  | 17012351
TRPC3 |  | NCX1 | Co-immunofluorescence staining | Rat cardiomyocytes |  |  |  |  |  | 17129578
TRPC3 |  | NCX1 | Co-immunofluorescence staining | dnTRPC3 TG mouse heart myocyte |  |  |  |  |  | 20351294
TRPC3 |  | NCX1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 14736881
TRPC3 |  | NCX1 | Co-immunoprecipitation |  | Sf9 | Mouse | Not specified | Rat | Not specified | 17012351
TRPC3 |  | NCX1 | Co-immunoprecipitation | Rat ventricle lysates |  |  |  |  |  | 17012351
TRPC3 |  | NCX1 | Co-immunoprecipitation | Rat cardiomyocytes |  |  |  |  |  | 17129578

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | NCX1 | Fusion protein-pull down assay | Human | 670-848 | Rat | Not determined |  |  | 17129578

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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