# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Klotho / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (8)
- Disease (2)

Name: Klotho (Klotho)
Official Symbol: KL provided by HGNC
Function:
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 9365 | Q9UEF7 |  | Q9UEF7 |  | 114766 | Q9UEF7 |  | hsa:9365 | 604824
Mouse | 16591 | O35082 | DIP-39894N | O35082 | MINT-8175062 | 200958 | O35082 |  | mmu:16591 |
Rat | 83504 | Q9Z2Y9 |  |  |  | 249723 | Q9Z2Y9 |  | rno:83504 |

PPI pairs:

TRPV5 | TRPV6

Biological Process:

- GO:0002526 : acute inflammatory response
- GO:0007568 : aging
- GO:0005975 : carbohydrate metabolic process
- GO:0006112 : energy reserve metabolic process
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0008286 : insulin receptor signaling pathway
- GO:0030501 : positive regulation of bone mineralization
- GO:0090080 : positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:604824 : Coronary artery disease, susceptibility to (OMIM: )
- OMIM:604824 : Tumoral calcinosis, hyperphosphatemic (OMIM:211900 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV5 |  | Klotho | Inference |  |  |  |  |  | Prediction | 16239475
TRPV6 |  | Klotho | Inference |  |  |  |  |  | Prediction | 16239475

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | Klotho | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16239475
TRPV5 |  | Klotho | Co-immunoprecipitation |  | mIMCD-3 | Human | Full-length | Mouse | Full-length | 23747339

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV5 |  | Klotho | Calcium measurement |  |  | Activation | 16239475
TRPV5 |  | Klotho | Calcium measurement |  |  | Activation | 18495742
TRPV5 |  | Klotho | Cell surface biotinylation |  | Increase in plasma membrane level |  | 16239475
TRPV5 |  | Klotho | Cell surface biotinylation |  | Increase in plasma membrane level |  | 18606998
TRPV5 |  | Klotho | In vitro PTM assay | Glycosylation (Asn-358) |  |  | 16239475
TRPV5 |  | Klotho | Patch clamp |  |  | Activation | 16239475
TRPV6 |  | Klotho | Calcium measurement |  |  | Activation | 18495742
TRPV6 |  | Klotho | In vitro PTM assay | Glycosylation (Asn-357) |  |  | 16239475
TRPV6 |  | Klotho | Patch clamp |  |  | Activation | 16239475

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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