# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Caveolin-1 / TRP channels

- Basic Information
- External Database
- PPI pairs (4)
- Biological Process (67)
- Disease (1)

Name: Caveolin-1 (Caveolin-1)
Official Symbol: CAV1 provided by HGNC
Function:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 857 | Q03135 | DIP-5960N | Q03135 | MINT-152933 | 107305 | Q03135 |  | hsa:857 | 601047
Mouse | 12389 | P49817 | DIP-35140N | P49817 | MINT-1542313 | 198514 | P49817 |  | mmu:12389 |
Rat | 25404 | P41350 | DIP-35143N | P41350 | MINT-202594 | 247439 | P41350 |  | rno:25404 |

PPI pairs:

TRPC1 | TRPC3 | TRPC4 | TRPV4

Biological Process:

- GO:0097190 : apoptotic signaling pathway
- GO:0007596 : blood coagulation
- GO:0006816 : calcium ion transport
- GO:0070836 : caveola assembly
- GO:0072584 : caveolin-mediated endocytosis
- GO:0071455 : cellular response to hyperoxia
- GO:0009267 : cellular response to starvation
- GO:0033344 : cholesterol efflux
- GO:0042632 : cholesterol homeostasis
- GO:0030301 : cholesterol transport
- GO:0051480 : cytosolic calcium ion homeostasis
- GO:0000188 : inactivation of MAPK activity
- GO:0007595 : lactation
- GO:0050900 : leukocyte migration
- GO:0019915 : lipid storage
- GO:0032507 : maintenance of protein location in cell
- GO:0060056 : mammary gland involution
- GO:0000165 : MAPK cascade
- GO:0051899 : membrane depolarization
- GO:0046785 : microtubule polymerization
- GO:2000811 : negative regulation of anoikis
- GO:0030514 : negative regulation of BMP signaling pathway
- GO:0090090 : negative regulation of canonical Wnt receptor signaling pathway
- GO:0001960 : negative regulation of cytokine-mediated signaling pathway
- GO:0001937 : negative regulation of endothelial cell proliferation
- GO:0030857 : negative regulation of epithelial cell differentiation
- GO:0046426 : negative regulation of JAK-STAT cascade
- GO:0045019 : negative regulation of nitric oxide biosynthetic process
- GO:0033137 : negative regulation of peptidyl-serine phosphorylation
- GO:0032091 : negative regulation of protein binding
- GO:0031397 : negative regulation of protein ubiquitination
- GO:0000122 : negative regulation of transcription from RNA polymerase II promoter
- GO:0042524 : negative regulation of tyrosine phosphorylation of Stat5 protein
- GO:0033484 : nitric oxide homeostasis
- GO:0046209 : nitric oxide metabolic process
- GO:0010524 : positive regulation of calcium ion transport into cytosol
- GO:0090263 : positive regulation of canonical Wnt receptor signaling pathway
- GO:0045807 : positive regulation of endocytosis
- GO:2001238 : positive regulation of extrinsic apoptotic signaling pathway
- GO:2001244 : positive regulation of intrinsic apoptotic signaling pathway
- GO:0048554 : positive regulation of metalloenzyme activity
- GO:0031116 : positive regulation of microtubule polymerization
- GO:0033138 : positive regulation of peptidyl-serine phosphorylation
- GO:0045907 : positive regulation of vasoconstriction
- GO:0051260 : protein homooligomerization
- GO:2000286 : receptor internalization involved in canonical Wnt receptor signaling pathway
- GO:0030193 : regulation of blood coagulation
- GO:0019217 : regulation of fatty acid metabolic process
- GO:0050999 : regulation of nitric-oxide synthase activity
- GO:0006940 : regulation of smooth muscle contraction
- GO:0051592 : response to calcium ion
- GO:0042493 : response to drug
- GO:0043627 : response to estrogen stimulus
- GO:0010332 : response to gamma radiation
- GO:0051384 : response to glucocorticoid stimulus
- GO:0001666 : response to hypoxia
- GO:0009612 : response to mechanical stimulus
- GO:0007584 : response to nutrient
- GO:0032570 : response to progesterone stimulus
- GO:0007519 : skeletal muscle tissue development
- GO:0044281 : small molecule metabolic process
- GO:0031295 : T cell costimulation
- GO:0006641 : triglyceride metabolic process
- GO:0001570 : vasculogenesis
- GO:0042310 : vasoconstriction
- GO:0016050 : vesicle organization
- GO:0019048 : virus-host interaction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:601047 : Lipodystrophy, congenital generalized, type 3 (OMIM:612526 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | Caveolin-1 | Inference |  |  |  |  |  | Prediction | 10766822
TRPC3 |  | Caveolin-1 | Inference |  |  |  |  |  | Prediction | 11524429
TRPC4 |  | Caveolin-1 | Inference |  |  |  |  |  | Prediction | 17416589
TRPV4 |  | Caveolin-1 | Inference |  |  |  |  |  | Prediction | 20705915

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC1 |  | Caveolin-1 | Fusion protein-pull down assay | Human | 1-347 | E. coli | Not specified | Full-length | HSG cell lysates | 12732636
TRPC1 |  | Caveolin-1 | Yeast two-hybrid | Not specified | 317-347 | Yeast | Not specified | Full-length | Yeast | 19897728
TRPC4 |  | Caveolin-1 | Fusion protein-pull down assay | Not used |  | Mouse lung endothelium cell lysates | Not specified | Full-length | E. coli | 17416589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | Caveolin-1 | Co-immunofluorescence staining |  | HUVEC | Human | Full-length | Mouse | Full-length | 20705915
TRPC1 |  | Caveolin-1 | Co-immunofluorescence staining | Rat caudal artery section |  |  |  |  |  | 14551243
TRPC1 |  | Caveolin-1 | Co-immunofluorescence staining |  | HSG cell | Not used |  | Not specified | Full-length | 12732636
TRPC1 |  | Caveolin-1 | Co-immunofluorescence staining |  | HSG cell | Human | Full-length | Not specified | Full-length | 12732636
TRPC1 |  | Caveolin-1 | Co-immunofluorescence staining |  | MDCK | Human | Full-length | Not specified | Full-length | 12732636
TRPC1 |  | Caveolin-1 | Co-immunofluorescence staining | HSG cell |  |  |  |  |  | 19897728
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation | C2C12 |  |  |  |  |  | 19351713
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation | Rat caudal artery cell |  |  |  |  |  | 14551243
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation | HSG cell |  |  |  |  |  | 18430726
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation |  | HSG cell | Human | Full-length | Not used |  | 10766822
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation |  | MDCK | Human | Full-length | Not specified | Full-length | 12732636
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation | HSG cell |  |  |  |  |  | 19897728
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation |  | HSG cell | Human | Full-length | Not used |  | 10980191
TRPC1 |  | Caveolin-1 | Fluorescence resonance energy transfer |  | HSG cell | Not specified | Full-length | Not specified | Full-length | 19897728
TRPC3 |  | Caveolin-1 | Co-immunofluorescence staining |  | HEK293 | Human | Full-length | Not used |  | 11524429
TRPC3 |  | Caveolin-1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 11524429
TRPC4 |  | Caveolin-1 | Co-immunofluorescence staining | Mouse lung endothelium cell |  |  |  |  |  | 17416589
TRPC4 |  | Caveolin-1 | Co-immunoprecipitation | Mouse lung endothelium cell |  |  |  |  |  | 17416589
TRPV4 |  | Caveolin-1 | Co-immunofluorescence staining |  | HUVEC | Human | Full-length | Mouse | Full-length | 20705915

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation | Human | 781-789 | Human | Not determined |  |  | 16822931
TRPC1 |  | Caveolin-1 | Co-immunoprecipitation | Human | 781-789 | Human | 82-101 |  |  | 19052258
TRPC1 |  | Caveolin-1 | Fusion protein-pull down assay | Human | Not determined | Human | 82-101 |  |  | 16822931
TRPC1 |  | Caveolin-1 | Yeast two-hybrid | Human | 271-349 | Not specified | Not determined |  |  | 12732636
TRPC4 |  | Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 61-101 |  |  | 17416589
TRPC4 |  | Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 135-178 |  |  | 17416589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC1 |  | Caveolin-1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 19897728

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.