# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# NCS-1 / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (3)
- Disease (0)

Name: Neuronal calcium sensor 1 (NCS-1)
Official Symbol: NCS1 provided by HGNC
Function:
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 23413 | P62166 |  | P62166 | MINT-1436213 | 116985 | P62166 |  | hsa:23413 | 603315
Mouse | 14299 | Q8BNY6 |  |  | MINT-4439526 | 199742 | Q8BNY6 |  | mmu:14299 |
Rat | 65153 | P62168 | DIP-35474N | P62168 | UNK-5381916 | 249269 | P62168 |  | rno:65153 |

PPI pairs:

TRPC1 | TRPC5

Biological Process:

- GO:0048015 : phosphatidylinositol-mediated signaling
- GO:0045921 : positive regulation of exocytosis
- GO:0010975 : regulation of neuron projection development

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC1 |  | NCS-1 | Inference |  |  |  |  |  | Prediction | 18261457
TRPC5 |  | NCS-1 | Inference |  |  |  |  |  | Prediction | 16469785

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC1 |  | NCS-1 | Fusion protein-pull down assay | Human | 639-759 | E. coli | Human | Full-length | E. coli | 18261457
TRPC5 |  | NCS-1 | Fusion protein-pull down assay | Human | 619-973 | E. coli | Human | Full-length | E. coli | 16469785
TRPC5 |  | NCS-1 | Yeast two-hybrid | Human | 619-973 | Yeast | Human | Full-length | Yeast | 16469785

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC5 |  | NCS-1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 16469785
TRPC5 |  | NCS-1 | Co-immunoprecipitation | Rat brain membrane lysates |  |  |  |  |  | 16469785

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC5 |  | NCS-1 | Fusion protein-pull down assay | Human | 619-803 | Human | Not determined |  |  | 16469785
TRPC5 |  | NCS-1 | Fusion protein-pull down assay | Human | 803-973 | Human | Not determined |  |  | 16469785
TRPC5 |  | NCS-1 | Yeast two-hybrid | Human | 619-803 | Human | Not determined |  |  | 16469785
TRPC5 |  | NCS-1 | Yeast two-hybrid | Human | 803-973 | Human | Not determined |  |  | 16469785

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPC5 |  | NCS-1 | Calcium measurement |  |  | Activation | 16469785
TRPC5 |  | NCS-1 | Patch clamp |  |  | Activation | 16469785

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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