# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# PTP1B / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (11)
- Disease (1)

Name: Tyrosine-protein phosphatase non-receptor type 1 (PTP1B)
Official Symbol: PTPN1 provided by HGNC
Function:
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII-and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 5770 | P18031 | DIP-38014N | P18031 | MINT-441655 | 111736 | P18031 |  | hsa:5770 | 176885
Mouse | 19246 | P35821 | DIP-32452N | P35821 | MINT-5313580 | 202476 | P35821 |  | mmu:19246 |
Rat | 24697 | P20417 | DIP-37289N | P20417 | UNK-5373400 | 246828 | P20417 |  | rno:24697 |

PPI pairs:

TRPP1 | TRPV6

Biological Process:

- GO:0031532 : actin cytoskeleton reorganization
- GO:0030968 : endoplasmic reticulum unfolded protein response
- GO:0008286 : insulin receptor signaling pathway
- GO:0060333 : interferon-gamma-mediated signaling pathway
- GO:0060397 : JAK-STAT cascade involved in growth hormone signaling pathway
- GO:0046627 : negative regulation of insulin receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0030100 : regulation of endocytosis
- GO:0060334 : regulation of interferon-gamma-mediated signaling pathway
- GO:0060338 : regulation of type I interferon-mediated signaling pathway
- GO:0060337 : type I interferon-mediated signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:176885 : Insulin resistance, susceptibility to (OMIM:125853 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPP1 |  | PTP1B | Affinity purification-mass spectrometry | Mouse | Not specified | Mouse | Vascular smooth muscle | MOVAS lysates |  | 19879844
TRPV6 |  | PTP1B | Inference |  |  |  |  |  | Prediction | 15894168

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV6 |  | PTP1B | Yeast two-hybrid | Rat | 1-326 | Yeast | Human | Full-length | Yeast | 17197020

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV6 |  | PTP1B | Biomolecular fluorescence complementation |  | HEK293 | Rat | Full-length | Human | Full-length | 15894168
TRPV6 |  | PTP1B | Biomolecular fluorescence complementation |  | HEK293 | Rat | Full-length | Human | Full-length | 17197020

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV6 |  | PTP1B | Biomolecular fluorescence complementation | Rat | 1-191 | Human | Not determined |  |  | 17197020

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV6 |  | PTP1B | Calcium measurement |  |  | Inhibition | 15894168
TRPV6 |  | PTP1B | Calcium measurement |  |  | Inhibition | 17197020
TRPV6 |  | PTP1B | In vitro PTM assay | Dephosphorylation |  |  | 15894168
TRPV6 |  | PTP1B | In vivo PTM assay | Dephosphorylation (Tyr-162) |  |  | 17197020
TRPV6 |  | PTP1B | In vivo PTM assay | Dephosphorylation (Tyr-161) |  |  | 17197020

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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