# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPV6 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (20)
- Biological Process (1)
- Disease (0)

Name: Transient receptor potential cation channel subfamily V member 6 (TRPV6)
Official Symbol: TRPV6 provided by HGNC
Function:
Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 55503 | Q9H1D0 | DIP-43888N |  | MINT-1472896 | 120683 | Q9H1D0 | 512 | hsa:55503 | 606680
Mouse | 64177 | Q91WD2 |  |  |  | 211037 | Q91WD2 |  | mmu:64177 |
Rat | 114246 | Q9R186 | DIP-47769N |  | UNK-5555281 | 250323 | Q9R186 |  | rno:114246 |

PPI pairs:

BSPRY | Calbindin-D28K | Calmodulin | Cyclophilin B | FYN | I-MFA | Klotho | NHERF4
Nipsnap1 | Numb | PTEN | PTP1B | RGS2 | Rab11a | RyR1 | S100A10
Src | TRPC1 | TRPML3 | TRPV5

Biological Process:

- GO:0017158 : regulation of calcium ion-dependent exocytosis

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV6 |  | BSPRY | Yeast two-hybrid | Mouse | 597-601 | Mouse | Kidney | cDNA library |  | 16380433
TRPV6 |  | Calbindin-D28K | Inference |  |  |  |  |  | Prediction | 16763551
TRPV6 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 11248124
TRPV6 |  | Cyclophilin B | Affinity purification-mass spectrometry | Human | 1-725 | Human | Placenta | Microsomal membrane fraction |  | 18445599
TRPV6 |  | FYN | Inference |  |  |  |  |  | Prediction | 17197020
TRPV6 |  | I-MFA | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified |  | 16189514
TRPV6 |  | Klotho | Inference |  |  |  |  |  | Prediction | 16239475
TRPV6 |  | NHERF4 | Yeast two-hybrid | Mouse | 575-727 | Mouse | Kidney | cDNA library |  | 17645868
TRPV6 |  | NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 16565876
TRPV6 |  | Nipsnap1 | Inference |  |  |  |  |  | Prediction | 18392847
TRPV6 |  | Numb | Inference |  |  |  |  |  | Prediction | 23140583
TRPV6 |  | PTEN | Inference |  |  |  |  |  | Prediction | 24704446
TRPV6 |  | PTP1B | Inference |  |  |  |  |  | Prediction | 15894168
TRPV6 |  | Rab11a | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 16354700
TRPV6 |  | RGS2 | Yeast two-hybrid | Mouse | 1-326 | Mouse | Kidney | cDNA library |  | 16895908
TRPV6 |  | RyR1 | Affinity purification-mass spectrometry | Not used as a bait |  | Rabbit | Fast-twitch muscle | Triad vesicles |  | 18215135
TRPV6 |  | S100A10 | Inference |  |  |  |  |  | Prediction | 12660155
TRPV6 |  | S100A10 | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified |  | 16189514
TRPV6 |  | SRC | Inference |  |  |  |  |  | Prediction | 17197020
TRPV6 |  | TRPC1 | Inference |  |  |  |  |  | Prediction | 22932896
TRPV6 |  | TRPML3 | Inference |  |  |  |  |  | Prediction | 23469151
TRPV6 |  | TRPV5 | Inference |  |  |  |  |  | Prediction | 12574114

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV6 |  | BSPRY | Fusion protein-pull down assay | Mouse | 597-601 | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 16380433
TRPV6 |  | BSPRY | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 16380433
TRPV6 |  | Calbindin-D28K | Fusion protein-pull down assay | Mouse | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551
TRPV6 |  | Calbindin-D28K | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Human | 584-725 | In vitro translation | Bovine | Full-length | E. coli | 11248124
TRPV6 |  | NHERF4 | Fusion protein-pull down assay | Mouse | 575-727 | E. coli | Mouse | Full-length | In vitro translation | 17645868
TRPV6 |  | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876
TRPV6 |  | Nipsnap1 | Fusion protein-pull down assay | Mouse | 577-607 | E. coli | Not specified | Full-length | E. coli | 18392847
TRPV6 |  | PTP1B | Yeast two-hybrid | Rat | 1-326 | Yeast | Human | Full-length | Yeast | 17197020
TRPV6 |  | Rab11a | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 16354700
TRPV6 |  | RGS2 | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Human | Full-length | E. coli | 16895908
TRPV6 |  | S100A10 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 12660155

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV6 |  | Calmodulin | Co-immunoprecipitation |  | Xenopus oocyte | Rabbit | Full-length | Not specified | Full-length | 15123711
TRPV6 |  | Calmodulin | Fluorescence resonance energy transfer |  | HEK293 | Rat | Full-length | Not specified | Full-length | 16959851
TRPV6 |  | Cyclophilin B | Co-immunofluorescence staining |  | Xenopus oocyte | Human | Full-length | Human | Full-length | 18445599
TRPV6 |  | Cyclophilin B | Co-immunofluorescence staining | Human placenta |  |  |  |  |  | 18445599
TRPV6 |  | Cyclophilin B | Co-immunoprecipitation | Human placenta |  |  |  |  |  | 18445599
TRPV6 |  | NHERF4 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876
TRPV6 |  | Nipsnap1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not specified | Full-length | 18392847
TRPV6 |  | Numb | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 24704446
TRPV6 |  | Numb | Co-immunoprecipitation | Du145 |  |  |  |  |  | 24704446
TRPV6 |  | Numb | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 23140583
TRPV6 |  | PTEN | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 24704446
TRPV6 |  | PTEN | Co-immunoprecipitation | Du145 |  |  |  |  |  | 24704446
TRPV6 |  | PTP1B | Biomolecular fluorescence complementation |  | HEK293 | Rat | Full-length | Human | Full-length | 15894168
TRPV6 |  | PTP1B | Biomolecular fluorescence complementation |  | HEK293 | Rat | Full-length | Human | Full-length | 17197020
TRPV6 |  | Rab11a | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16354700
TRPV6 |  | Rab11a | Co-immunofluorescence staining |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700
TRPV6 |  | S100A10 | Co-immunofluorescence staining | Rat duodenum tissue |  |  |  |  |  | 12660155
TRPV6 |  | S100A10 | Co-immunoprecipitation | Caco-2 cell |  |  |  |  |  | 18187190
TRPV6 |  | S100A10 | Co-immunoprecipitation | Human 16HBE14o- cell |  |  |  |  |  | 18187190
TRPV6 |  | SRC | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Human | Full-length | 17197020
TRPV6 |  | TRPC1 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Human | Full-length | 22932896
TRPV6 |  | TRPC1 | Co-immunoprecipitation | LNCaP |  |  |  |  |  | 25172921
TRPV6 |  | TRPC1 | Fluorescence resonance energy transfer |  | HEK293 | Rat | Full-length | Human | Full-length | 22932896
TRPV6 |  | TRPML3 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151
TRPV6 |  | TRPV5 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 12574114
TRPV6 |  | TRPV5 | Co-immunoprecipitation | Human myeloid leukemia K562 cell |  |  |  |  |  | 19295174
TRPV6 |  | TRPV5 | Co-immunoprecipitation |  | Xenopus oocyte | Mouse | Full-length | Rat | Full-length | 12574114
TRPV6 |  | TRPV5 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749
TRPV6 |  | TRPV5 | Fluorescence resonance energy transfer |  | HEK293 | Not specified | Full-length | Mouse | Full-length | 23469151
TRPV6 |  | TRPV5 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV6 |  | Calmodulin | Fluorescence probe labeling |  |  |  |  | 1:1 | 43 nM | 12765696
TRPV6 |  | Calmodulin | Fluorescence probe labeling |  |  |  |  |  | 65 nM | 11248124
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | 327-577 | Not specified | Not determined |  |  | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | 88-97 | Not specified | Not determined |  |  | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Rabbit | 643-656 | Not specified | Not determined |  |  | 15123711
TRPV6 |  | Calmodulin | Fusion protein-pull down assay | Human | 691-711 | Bovine | Not determined |  |  | 11248124
TRPV6 |  | NHERF4 | Fusion protein-pull down assay | Mouse | 724-727 | Mouse | Not determined |  |  | 17645868
TRPV6 |  | Nipsnap1 | Fusion protein-pull down assay | Mouse | 83-118 | Not specified | Not determined |  |  | 18392847
TRPV6 |  | Numb | Co-immunoprecipitation | Human | 716 | Human | 434 |  |  | 23140583
TRPV6 |  | PTP1B | Biomolecular fluorescence complementation | Rat | 1-191 | Human | Not determined |  |  | 17197020
TRPV6 |  | S100A10 | Fusion protein-pull down assay | Mouse | 597-601 | Mouse | Not determined |  |  | 12660155
TRPV6 |  | TRPC1 | Fluorescence resonance energy transfer | Rat | 1-198 | Human | 1-193 |  |  | 22932896

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV6 |  | Calmodulin | Calcium measurement |  |  | Inhibition | 16959851
TRPV6 |  | Calmodulin | Patch clamp |  |  | Inhibition | 15123711
TRPV6 |  | Calmodulin | Patch clamp |  |  | Inhibition | 11248124
TRPV6 |  | Cyclophilin B | Calcium measurement |  |  | Activation | 18445599
TRPV6 |  | FYN | In vitro PTM assay | Phosphorylation |  |  | 17197020
TRPV6 |  | Klotho | Calcium measurement |  |  | Activation | 18495742
TRPV6 |  | Klotho | In vitro PTM assay | Glycosylation (Asn-357) |  |  | 16239475
TRPV6 |  | Klotho | Patch clamp |  |  | Activation | 16239475
TRPV6 |  | NHERF4 | Patch clamp |  |  | Activation | 17645868
TRPV6 |  | Nipsnap1 | Patch clamp |  |  | Inhibition | 18392847
TRPV6 |  | Numb | Calcium measurement |  |  | Inhibition | 23140583
TRPV6 |  | PTP1B | Calcium measurement |  |  | Inhibition | 15894168
TRPV6 |  | PTP1B | Calcium measurement |  |  | Inhibition | 17197020
TRPV6 |  | PTP1B | In vitro PTM assay | Dephosphorylation |  |  | 15894168
TRPV6 |  | PTP1B | In vivo PTM assay | Dephosphorylation (Tyr-162) |  |  | 17197020
TRPV6 |  | PTP1B | In vivo PTM assay | Dephosphorylation (Tyr-161) |  |  | 17197020
TRPV6 |  | Rab11a | Calcium measurement |  |  | Activation | 16354700
TRPV6 |  | Rab11a | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 16354700
TRPV6 |  | RGS2 | Patch clamp |  |  | Inhibition | 16895908
TRPV6 |  | S100A10 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 12660155
TRPV6 |  | S100A10 | Patch clamp |  |  | Activation | 12660155
TRPV6 |  | SRC | In vitro PTM assay | Phosphorylation |  |  | 17197020
TRPV6 |  | SRC | In vivo PTM assay | Phosphorylation (Tyr-162) |  |  | 17197020
TRPV6 |  | SRC | In vivo PTM assay | Phosphorylation (Tyr-161) |  |  | 17197020
TRPV6 |  | TRPC1 | Cell surface biotinylation |  | Retain in the endoplasmic reticulum |  | 22932896
TRPV6 |  | TRPC1 | Patch clamp |  |  | Inhibition | 22932896
TRPV6 |  | TRPV5 | Patch clamp |  |  | New channel creation | 12574114

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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