# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# eNOS / TRP channels

- Basic Information
- External Database
- PPI pairs (1)
- Biological Process (47)
- Disease (6)

Name: Nitric oxide synthase, endothelial (eNOS)
Official Symbol: NOS3 provided by HGNC
Function:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 4846 | P29474 | DIP-38479N | P29474 | MINT-106980 | 110909 | P29474 |  | hsa:4846 | 163729
Mouse | 18127 | P70313 | DIP-31086N | P70313 | UNK-5382667 | 201807 | P70313 |  | mmu:18127 |
Rat | 24600 | Q62600 | DIP-41833N |  | MINT-202608 | 246740 | Q62600 |  | rno:24600 |

PPI pairs:

TRPV1

Biological Process:

- GO:0007568 : aging
- GO:0001525 : angiogenesis
- GO:0006527 : arginine catabolic process
- GO:0007596 : blood coagulation
- GO:0001974 : blood vessel remodeling
- GO:0071560 : cellular response to transforming growth factor beta stimulus
- GO:0043542 : endothelial cell migration
- GO:0001701 : in utero embryonic development
- GO:0051701 : interaction with host
- GO:0031663 : lipopolysaccharide-mediated signaling pathway
- GO:0030324 : lung development
- GO:0007005 : mitochondrion organization
- GO:0043066 : negative regulation of apoptotic process
- GO:0045776 : negative regulation of blood pressure
- GO:0051926 : negative regulation of calcium ion transport
- GO:0008285 : negative regulation of cell proliferation
- GO:0051346 : negative regulation of hydrolase activity
- GO:0014740 : negative regulation of muscle hyperplasia
- GO:0010544 : negative regulation of platelet activation
- GO:0043267 : negative regulation of potassium ion transport
- GO:0048662 : negative regulation of smooth muscle cell proliferation
- GO:0006809 : nitric oxide biosynthetic process
- GO:0007263 : nitric oxide mediated signal transduction
- GO:0001542 : ovulation from ovarian follicle
- GO:0090382 : phagosome maturation
- GO:0045766 : positive regulation of angiogenesis
- GO:0043065 : positive regulation of apoptotic process
- GO:0031284 : positive regulation of guanylate cyclase activity
- GO:0045909 : positive regulation of vasodilation
- GO:0050880 : regulation of blood vessel size
- GO:0050999 : regulation of nitric-oxide synthase activity
- GO:0002028 : regulation of sodium ion transport
- GO:0003100 : regulation of systemic arterial blood pressure by endothelin
- GO:0043200 : response to amino acid stimulus
- GO:0034097 : response to cytokine stimulus
- GO:0042493 : response to drug
- GO:0032355 : response to estradiol stimulus
- GO:0045471 : response to ethanol
- GO:0034405 : response to fluid shear stress
- GO:0009408 : response to heat
- GO:0055093 : response to hyperoxia
- GO:0001666 : response to hypoxia
- GO:0010288 : response to lead ion
- GO:0055094 : response to lipoprotein particle stimulus
- GO:0009612 : response to mechanical stimulus
- GO:0043434 : response to peptide hormone stimulus
- GO:0014806 : smooth muscle hyperplasia

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:163729 : Coronary artery spasm 1, susceptibility to (OMIM: )
- OMIM:163729 : Alzheimer disease, late-onset, susceptibility to (OMIM:104300 )
- OMIM:163729 : Hypertension, susceptibility to (OMIM:145500 )
- OMIM:163729 : Hypertension, pregnancy-induced (OMIM:189800 )
- OMIM:163729 : Placental abruption (OMIM: )
- OMIM:163729 : Ischemic stroke, susceptibility to (OMIM:601367 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV1 |  | eNOS | Inference |  |  |  |  |  | Prediction | 21493704

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV1 |  | eNOS | Co-immunoprecipitation | Bovine aortic endothelial cells |  |  |  |  |  | 21493704
TRPV1 |  | eNOS | Co-immunoprecipitation | Bovine aortic endothelial cells |  |  |  |  |  | 22451268
TRPV1 |  | eNOS | Mammalian two-hybrid |  | Bovine aortic endothelial cells | Rat | Full-length | Human | Full-length | 22451268

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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