# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPP2 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (8)
- Biological Process (2)
- Disease (0)

Name: Polycystic kidney disease 2-like 1 protein (TRPP2)
Official Symbol: PKD2L1 provided by HGNC
Function:
May function as a subunit of an ion channel and act as a transducer of calcium-mediated signaling.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 9033 | Q9P0L9 |  |  | MINT-3973148 | 114499 | Q9P0L9 | 505 | hsa:9033 | 604532
Mouse | 329064 | A2A259 |  |  |  | 236700 |  |  | mmu:329064 |
Rat | 293937 | D3ZDT6 |  |  |  | 254514 |  |  | rno:293937 |

PPI pairs:

PKD1/Polycystin-1 | PKD1L3/Polycystin-1L3 | Troponin I 1 | Troponin I 2 | Troponin I 3 | ơ-actinin | ơ-actinin-1 | ơ-actinin-2

Biological Process:

- GO:0071468 : cellular response to acidity
- GO:0001581 : detection of chemical stimulus involved in sensory perception of sour taste

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPP2 |  | ơ-actinin | Inference |  |  |  |  |  | Prediction | 17944866
TRPP2 |  | ơ-actinin-1 | Inference |  |  |  |  |  | Prediction | 17944866
TRPP2 |  | ơ-actinin-2 | Inference |  |  |  |  |  | Prediction | 17944866
TRPP2 |  | PKD1/Polycystin-1 | Inference |  |  |  |  |  | Prediction | 15548533
TRPP2 |  | PKD1L3/Polycystin-1L3 | Inference |  |  |  |  |  | Prediction | 16891422
TRPP2 |  | Troponin I 1 | Inference |  |  |  |  |  | Prediction | 12809519
TRPP2 |  | Troponin I 2 | Inference |  |  |  |  |  | Prediction | 12809519
TRPP2 |  | Troponin I 3 | Inference |  |  |  |  |  | Prediction | 12809519

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPP2 |  | ơ-actinin | Fusion protein-pull down assay | Human | 1-97 | E. coli | Chicken | Full-length | In vitro translation | 17944866
TRPP2 |  | ơ-actinin | Fusion protein-pull down assay | Human | 562-805 | E. coli | Chicken | Full-length | In vitro translation | 17944866
TRPP2 |  | ơ-actinin-1 | Yeast two-hybrid | Human | 1-97 | Yeast | Human | 334-892 | Yeast | 17944866
TRPP2 |  | ơ-actinin-1 | Yeast two-hybrid | Human | 562-805 | Yeast | Human | 334-892 | Yeast | 17944866
TRPP2 |  | ơ-actinin-2 | Yeast two-hybrid | Human | 1-97 | Yeast | Human | 341-894 | Yeast | 17944866
TRPP2 |  | ơ-actinin-2 | Yeast two-hybrid | Human | 562-805 | Yeast | Human | 341-894 | Yeast | 17944866
TRPP2 |  | Troponin I 1 | Fusion protein-pull down assay | Human | 562-805 | E. coli | Human | Not specified | Not specified | 12809519
TRPP2 |  | Troponin I 2 | Fusion protein-pull down assay | Human | 562-805 | E. coli | Human | Not specified | Not specified | 12809519
TRPP2 |  | Troponin I 3 | Fusion protein-pull down assay | Human | 562-805 | E. coli | Human | Not specified | Not specified | 12809519

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP2 |  | ơ-actinin-1 | Co-immunoprecipitation | HEK293 |  |  |  |  |  | 17944866
TRPP2 |  | ơ-actinin-2 | Co-immunoprecipitation | Mouse brain lysates |  |  |  |  |  | 17944866
TRPP2 |  | PKD1/Polycystin-1 | Co-immunoprecipitation | mIMCD-3 |  |  |  |  |  | 16609150
TRPP2 |  | PKD1/Polycystin-1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 15548533
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunofluorescence staining | Mouse taste cell |  |  |  |  |  | 16891422
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 16891422
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 20538909
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunoprecipitation |  | Xenopus oocyte | Human | Full-length | Human | Full-length | 23212381
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 18535624
TRPP2 |  | Troponin I 1 | Co-immunoprecipitation |  | NIH-3T3 | Human | Full-length | Human | Full-length | 12809519
TRPP2 |  | Troponin I 1 | Co-immunoprecipitation |  | Xenopus oocyte | Human | Full-length | Human | Full-length | 12809519
TRPP2 |  | Troponin I 2 | Co-immunoprecipitation |  | NIH-3T3 | Human | Full-length | Human | Full-length | 12809519
TRPP2 |  | Troponin I 2 | Co-immunoprecipitation |  | Xenopus oocyte | Human | Full-length | Human | Full-length | 12809519
TRPP2 |  | Troponin I 3 | Co-immunoprecipitation |  | NIH-3T3 | Human | Full-length | Human | Full-length | 12809519
TRPP2 |  | Troponin I 3 | Co-immunoprecipitation |  | Xenopus oocyte | Human | Full-length | Human | Full-length | 12809519
TRPP2 |  | Troponin I 3 | Co-immunoprecipitation | Human adult cardiac tissue |  |  |  |  |  | 12809519

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP2 |  | ơ-actinin-2 | Yeast two-hybrid | Human | 1-97 | Human | 596-728 |  |  | 17944866
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | Mouse | Full-length | Mouse | 1594-2151 |  |  | 16891422
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | Mouse | Not determined | Mouse | 1896-2040 |  |  | 20538909
TRPP2 |  | PKD1L3/Polycystin-1L3 | Single-molecule photobleaching |  |  |  |  | 3:1 |  | 23212381
TRPP2 |  | Troponin I 3 | Yeast two-hybrid | Human | 630-705 | Human | Not determined |  |  | 12809519
TRPP2 |  | Troponin I 3 | Yeast two-hybrid | Human | 562-805 | Human | 1-170 |  |  | 12809519
TRPP2 |  | Troponin I 3 | Yeast two-hybrid | Human | 562-805 | Human | 106-158 |  |  | 12809519

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPP2 |  | ơ-actinin | Patch clamp |  |  | Activation | 17944866
TRPP2 |  | PKD1/Polycystin-1 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 16609150
TRPP2 |  | PKD1/Polycystin-1 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 15548533
TRPP2 |  | PKD1L3/Polycystin-1L3 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 20538909
TRPP2 |  | PKD1L3/Polycystin-1L3 | Patch clamp |  |  | New channel creation | 23212381
TRPP2 |  | PKD1L3/Polycystin-1L3 | Patch clamp |  |  | New channel creation | 18535624
TRPP2 |  | Troponin I 1 | Calcium measurement |  |  | Inhibition | 12809519
TRPP2 |  | Troponin I 1 | Patch clamp |  |  | Inhibition | 12809519
TRPP2 |  | Troponin I 3 | Calcium measurement |  |  | Inhibition | 12809519

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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