# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPV4 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (38)
- Biological Process (17)
- Disease (9)

Name: Transient receptor potential cation channel subfamily V member 4 (TRPV4)
Official Symbol: TRPV4 provided by HGNC
Function:
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 59341 | Q9HBA0 | DIP-35702N | Q9HBA0 | MINT-4535111 | 121883 | Q9HBA0 | 510 | hsa:59341 | 605427
Mouse | 63873 | Q9EPK8 | DIP-44011N |  | MINT-4050076 | 211003 | Q9EPK8 |  | mmu:63873 |
Rat | 66026 | Q9ERZ8 | DIP-59600N |  |  | 249353 | Q9ERZ8 |  | rno:66026 |

PPI pairs:

80K-H | AKAP5/AKAP150 | AQP-4 | AQP-5 | AT1aR | Bkca | Calmodulin | Caveolin-1
E-cadherin | FYN | HCK | IP3R3 | ITCH/AIP4 | LCK | LYN | MAP7
Myosin-10 | Myosin-HIIA | NHERF4 | OS-9 | PACS-1 | Pacsin1 | Pacsin2 | Pacsin3
RPS27A | Src | TMEM16A/ANO1 | TRPC1 | TRPP1 | Tubulin | UBC | YES
ơ2-integrin | Ƣ-arrestin 1 | Ƣ-arrestin 2 | Ƣ-catenin | Ƣ-tubulin-5 | ƣ-actin

Biological Process:

- GO:0031532 : actin cytoskeleton reorganization
- GO:0007015 : actin filament organization
- GO:0070509 : calcium ion import
- GO:0008219 : cell death
- GO:0006884 : cell volume homeostasis
- GO:0007043 : cell-cell junction assembly
- GO:0071476 : cellular hypotonic response
- GO:0043622 : cortical microtubule organization
- GO:0007204 : elevation of cytosolic calcium ion concentration
- GO:0042538 : hyperosmotic salinity response
- GO:0046785 : microtubule polymerization
- GO:0010977 : negative regulation of neuron projection development
- GO:0007231 : osmosensory signaling pathway
- GO:0031117 : positive regulation of microtubule depolymerization
- GO:0047484 : regulation of response to osmotic stress
- GO:0009612 : response to mechanical stimulus
- GO:0030103 : vasopressin secretion

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:605427 : Brachyolmia type 3 (OMIM:113500 )
- OMIM:605427 : Spondylometaphyseal dysplasia, Kozlowski type (OMIM:184252 )
- OMIM:605427 : Metatropic dysplasia (OMIM:156530 )
- OMIM:605427 : Hereditary motor and sensory neuropathy, type IIc (OMIM:606071 )
- OMIM:605427 : Scapuloperoneal spinal muscular atrophy (OMIM:181405 )
- OMIM:605427 : Sodium serum level QTL 1 (OMIM:613508 )
- OMIM:605427 : Parastremmatic dwarfism (OMIM:168400 )
- OMIM:605427 : SED, Maroteaux type (OMIM:184095 )
- OMIM:605427 : Spinal muscular atrophy, distal, congenital nonprogressive (OMIM:600175 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV4 |  | 80K-H | Speculation |  |  |  |  |  | Prediction | 19801576
TRPV4 |  | AKAP5/AKAP150 | Speculation |  |  |  |  |  | Prediction | 18701070
TRPV4 |  | AQP-4 | Inference |  |  |  |  |  | Prediction | 21262839
TRPV4 |  | AQP-5 | Inference |  |  |  |  |  | Prediction | 16571723
TRPV4 |  | AT1aR | Inference |  |  |  |  |  | Prediction | 20650893
TRPV4 |  | Bkca | Inference |  |  |  |  |  | Prediction | 25139746
TRPV4 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 12724311
TRPV4 |  | Caveolin-1 | Inference |  |  |  |  |  | Prediction | 20705915
TRPV4 |  | E-cadherin | Inference |  |  |  |  |  | Prediction | 20413591
TRPV4 |  | FYN | Inference |  |  |  |  |  | Prediction | 12538589
TRPV4 |  | ƣ-actin | Inference |  |  |  |  |  | Prediction | 22101010
TRPV4 |  | Ƣ-actin | Inference |  |  |  |  |  | Prediction | 19027987
TRPV4 |  | Ƣ-arrestin 1 | Inference |  |  |  |  |  | Prediction | 20650893
TRPV4 |  | Ƣ-arrestin 2 | Affinity purification-mass spectrometry | Not used as a bait |  | Human |  | HEK293 lysates |  | 17620599
TRPV4 |  | Ƣ-catenin | Yeast two-hybrid | Mouse | N-terminus | Human | Keratinocyte | cDNA library |  | 20413591
TRPV4 |  | Ƣ-tubulin-5 | Inference |  |  |  |  |  | Prediction | 22309793
TRPV4 |  | HCK | Inference |  |  |  |  |  | Prediction | 12538589
TRPV4 |  | IP3R3 | Inference |  |  |  |  |  | Prediction | 18378772
TRPV4 |  | ITCH/AIP4 | Inference |  |  |  |  |  | Prediction | 17110928
TRPV4 |  | LCK | Inference |  |  |  |  |  | Prediction | 12538589
TRPV4 |  | LYN | Inference |  |  |  |  |  | Prediction | 12538589
TRPV4 |  | MAP7 | Yeast two-hybrid | Mouse | 783-871 | Mouse | Kidney | cDNA library |  | 14517216
TRPV4 |  | Myosin-10 | Affinity purification-mass spectrometry | Mouse | Full-length | Human | HEK293 | Cell lystaes |  | 22492541
TRPV4 |  | Myosin-HIIA | Affinity purification-mass spectrometry | Mouse | Full-length | Human | HEK293 | Cell lystaes |  | 22492541
TRPV4 |  | NHERF4 | Inference |  |  |  |  |  | Prediction | 16565876
TRPV4 |  | ơ2-integrin | Inference |  |  |  |  |  | Prediction | 18234883
TRPV4 |  | OS-9 | Yeast two-hybrid | Mouse | 61-467 | Human | adult kidney | cDNA library |  | 17932042
TRPV4 |  | PACS-1 | Inference |  |  |  |  |  | Prediction | 15692563
TRPV4 |  | Pacsin1 | Yeast two-hybrid | Chicken | N-terminus | Chicken | Inner ear | cDNA library |  | 16627472
TRPV4 |  | Pacsin2 | Inference |  |  |  |  |  | Prediction | 16627472
TRPV4 |  | Pacsin3 | Yeast two-hybrid | Chicken | N-terminus | Chicken | Inner ear | cDNA library |  | 16627472
TRPV4 |  | RPS27A | Affinity purification-mass spectrometry | Mouse | Full-length | Human | HEK293 | Cell lystaes |  | 22492541
TRPV4 |  | SRC | Inference |  |  |  |  |  | Prediction | 12538589
TRPV4 |  | TMEM16A/ANO1 | Inference |  |  |  |  |  | Prediction | 24509911
TRPV4 |  | TRPC1 | Inference |  |  |  |  |  | Prediction | 20705915
TRPV4 |  | TRPP1 | Inference |  |  |  |  |  | Prediction | 18695040
TRPV4 |  | UBC | Inference |  |  |  |  |  | Prediction | 17110928
TRPV4 |  | YES | Inference |  |  |  |  |  | Prediction | 12538589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Not specified | Not specified | In vitro translation | 22492541
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | 718-871 | E. coli | Not used |  | HEK293T lysates | 21837266
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | C-terminus | E. coli | Not specified | Full-length | Not specified | 12724311
TRPV4 |  | ƣ-actin | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Not used |  | HEK293 lysates | 22101010
TRPV4 |  | MAP7 | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Mouse | Full-length | In vitro translation | 14517216
TRPV4 |  | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876
TRPV4 |  | Tubulin | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Not used |  | HEK293 lysates | 22101010

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV4 |  | 80K-H | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Human | Full-length | 19801576
TRPV4 |  | AKAP5/AKAP150 | Co-immunofluorescence staining | Rat cerebral artery |  |  |  |  |  | 24778429
TRPV4 |  | AKAP5/AKAP150 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not used |  | 18701070
TRPV4 |  | AQP-4 | Co-immunofluorescence staining | Adult rat brain section |  |  |  |  |  | 21262839
TRPV4 |  | AQP-4 | Co-immunoprecipitation | Adult rat brain lysates |  |  |  |  |  | 21262839
TRPV4 |  | AQP-4 | Co-immunoprecipitation |  | DI TNC1 | Human | Full-length | Rat | Full-length | 21262839
TRPV4 |  | AQP-5 | Co-immunoprecipitation | Mouse submandibular gland cell |  |  |  |  |  | 16571723
TRPV4 |  | AQP-5 | Co-immunoprecipitation | HSG cell |  |  |  |  |  | 16571723
TRPV4 |  | AT1aR | Co-immunofluorescence staining |  | Rat vascular smooth muscle cell | Human | Full-length | Human | Full-length | 20650893
TRPV4 |  | AT1aR | Co-immunoprecipitation |  | Rat vascular smooth muscle cell | Not used |  | Human | Full-length | 20650893
TRPV4 |  | AT1aR | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 20650893
TRPV4 |  | Bkca | Co-immunoprecipitation | Porcine coronary artery |  |  |  |  |  | 25139746
TRPV4 |  | Caveolin-1 | Co-immunofluorescence staining |  | HUVEC | Human | Full-length | Mouse | Full-length | 20705915
TRPV4 |  | E-cadherin | Co-immunoprecipitation | MDCK |  |  |  |  |  | 20413591
TRPV4 |  | E-cadherin | Co-immunoprecipitation | HEK |  |  |  |  |  | 22374181
TRPV4 |  | FYN | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 12538589
TRPV4 |  | Ƣ-actin | Co-immunofluorescence staining |  | HaCaT | Human | Full-length | Not used |  | 19027987
TRPV4 |  | ƣ-actin | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 22101010
TRPV4 |  | Ƣ-actin | Fluorescence resonance energy transfer |  | CHO | Human | Full-length | Human | Full-length | 19027987
TRPV4 |  | Ƣ-arrestin 1 | Co-immunoprecipitation | Rat vascular smooth muscle cell |  |  |  |  |  | 20650893
TRPV4 |  | Ƣ-arrestin 1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 20650893
TRPV4 |  | Ƣ-catenin | Co-immunoprecipitation | MDCK |  |  |  |  |  | 20413591
TRPV4 |  | Ƣ-catenin | Co-immunoprecipitation |  | COS-7 | Mouse | Full-length | Not specified | Not specified | 20413591
TRPV4 |  | Ƣ-catenin | Co-immunoprecipitation | HEK |  |  |  |  |  | 22374181
TRPV4 |  | Ƣ-tubulin-5 | Co-immunofluorescence staining |  | HEK293 | Rat | Full-length | Rat | Full-length | 22309793
TRPV4 |  | Ƣ-tubulin-5 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Rat | Full-length | 22309793
TRPV4 |  | HCK | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 12538589
TRPV4 |  | IP3R3 | Co-immunoprecipitation |  | HeLa | Human | Full-length | Rat | Full-length | 18378772
TRPV4 |  | IP3R3 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Rat | Full-length | 18826956
TRPV4 |  | ITCH/AIP4 | Co-immunofluorescence staining |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 17110928
TRPV4 |  | ITCH/AIP4 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 20650893
TRPV4 |  | ITCH/AIP4 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 17110928
TRPV4 |  | LCK | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 12538589
TRPV4 |  | LYN | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Not used |  | 12538589
TRPV4 |  | LYN | Co-immunoprecipitation | Rat dorsal root ganglion neuron |  |  |  |  |  | 18234883
TRPV4 |  | LYN | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 12538589
TRPV4 |  | MAP7 | Co-immunofluorescence staining | Mouse lung section |  |  |  |  |  | 14517216
TRPV4 |  | MAP7 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 14517216
TRPV4 |  | MAP7 | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 14517216
TRPV4 |  | MAP7 | Co-immunoprecipitation | Mouse renal tissue |  |  |  |  |  | 14517216
TRPV4 |  | MAP7 | Co-immunoprecipitation |  | HEK293 | Mouse | 785-808 | Mouse | Full-length | 14517216
TRPV4 |  | Myosin-10 | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Not used |  | 22492541
TRPV4 |  | Myosin-HIIA | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Not used |  | 22492541
TRPV4 |  | ơ2-integrin | Co-immunoprecipitation | Rat dorsal root ganglion neuron |  |  |  |  |  | 18234883
TRPV4 |  | OS-9 | Co-immunofluorescence staining |  | SJSA-1 | Mouse | Full-length | Not used |  | 17932042
TRPV4 |  | OS-9 | Co-immunoprecipitation |  | HEK293T | Mouse | 61-467 | Human | 430-667 | 17932042
TRPV4 |  | PACS-1 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 15692563
TRPV4 |  | Pacsin1 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin2 | Co-immunofluorescence staining |  | HEK293 | Rat | Full-length | Mouse | Full-length | 18174177
TRPV4 |  | Pacsin2 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin2 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin3 | Co-immunofluorescence staining | Mouse kidney renal tubule |  |  |  |  |  | 16627472
TRPV4 |  | Pacsin3 | Co-immunofluorescence staining |  | NIH-3T3 | Rat | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin3 | Co-immunofluorescence staining |  | HEK293 | Rat | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin3 | Co-immunofluorescence staining |  | HEK293 | Rat | Full-length | Mouse | Full-length | 18174177
TRPV4 |  | Pacsin3 | Co-immunoprecipitation | Mouse kidney tissue |  |  |  |  |  | 18174177
TRPV4 |  | Pacsin3 | Co-immunoprecipitation |  | HEK293 | Rat | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin3 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16627472
TRPV4 |  | Pacsin3 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 23690576
TRPV4 |  | SRC | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 12538589
TRPV4 |  | TMEM16A/ANO1 | Co-immunoprecipitation |  | HEK293T | Mouse | Full-length | Mouse | Full-length | 24509911
TRPV4 |  | TRPC1 | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Human | Full-length | 20093626
TRPV4 |  | TRPC1 | Co-immunofluorescence staining | Rat mesenteric artery endothelial cell lysates |  |  |  |  |  | 20093626
TRPV4 |  | TRPC1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 20093626
TRPV4 |  | TRPC1 | Co-immunoprecipitation | Rat mesenteric artery endothelial cell lysates |  |  |  |  |  | 20093626
TRPV4 |  | TRPC1 | Co-immunoprecipitation |  | Rat mesenteric artery endothelial cell | Mouse | Full-length | Human | Full-length | 25114176
TRPV4 |  | TRPC1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 25114176
TRPV4 |  | TRPC1 | Co-immunoprecipitation | Porcine coronary artery |  |  |  |  |  | 25139746
TRPV4 |  | TRPC1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 20093626
TRPV4 |  | TRPC1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 20705915
TRPV4 |  | TRPC1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 21871867
TRPV4 |  | TRPC1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 25114176
TRPV4 |  | TRPP1 | Co-immunofluorescence staining | MDCK |  |  |  |  |  | 18695040
TRPV4 |  | TRPP1 | Co-immunofluorescence staining |  | HEK293 | Mouse | Full-length | Human | Full-length | 22518003
TRPV4 |  | TRPP1 | Co-immunofluorescence staining |  | CRL-2038 (Mouse renal cortical collecting duct cell line / M1-CCD) | Mouse | Full-length | Human | Full-length | 22518003
TRPV4 |  | TRPP1 | Co-immunofluorescence staining | Mouse orpk collecting duct cell line |  |  |  |  |  | 23977387
TRPV4 |  | TRPP1 | Co-immunoprecipitation |  | HEK293 tsA 201 | Mouse | Full-length | Human | Full-length | 20682256
TRPV4 |  | TRPP1 | Co-immunoprecipitation |  | Rat mesenteric artery endothelial cell | Mouse | Full-length | Human | Full-length | 25114176
TRPV4 |  | TRPP1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 25114176
TRPV4 |  | TRPP1 | Co-immunoprecipitation |  | HEK293 | Mouse | C-terminus | Human | C-terminus | 18695040
TRPV4 |  | TRPP1 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Human | Full-length | 22518003
TRPV4 |  | TRPP1 | Co-immunoprecipitation |  | CRL-2038 (Mouse renal cortical collecting duct cell line / M1-CCD) | Mouse | Full-length | Human | Full-length | 22518003
TRPV4 |  | TRPP1 | Co-immunoprecipitation | Mouse inner medullary collecting duct cell |  |  |  |  |  | 23977387
TRPV4 |  | TRPP1 | Co-immunoprecipitation | Mouse orpk collecting duct cell line |  |  |  |  |  | 23977387
TRPV4 |  | TRPP1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 25114176
TRPV4 |  | TRPP1 | Fluorescence resonance energy transfer |  | HEK293 | Mouse | Full-length | Human | Full-length | 18695040
TRPV4 |  | Tubulin | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 22101010
TRPV4 |  | YES | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Not used |  | 12538589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined |  |  | 20926660
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Chicken | 132-383 | Not specified | Not determined |  |  | 19864432
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | 718-871 | Human | Not determined |  |  | 21837266
TRPV4 |  | Calmodulin | Fusion protein-pull down assay | Human | 812-831 | Not specified | Not determined |  |  | 12724311
TRPV4 |  | FYN | Co-immunoprecipitation | Mouse | Not determined | Mouse | 145-247 |  |  | 12538589
TRPV4 |  | ƣ-actin | Fusion protein-pull down assay | Mouse | 718-871 | Human | Not determined |  |  | 22101010
TRPV4 |  | Ƣ-catenin | Co-immunoprecipitation | Mouse | Not determined | Not specified | Not determined |  |  | 20413591
TRPV4 |  | IP3R3 | Co-immunoprecipitation | Human | 812-831 | Rat | Not determined |  |  | 18826956
TRPV4 |  | OS-9 | Co-immunoprecipitation | Mouse | 438-468 | Human | 430-667 |  |  | 17932042
TRPV4 |  | PACS-1 | Co-immunoprecipitation | Human | 1-486 | Human | Not determined |  |  | 15692563
TRPV4 |  | Pacsin3 | Co-immunoprecipitation | Mouse | 132-144 | Mouse | 368-424 |  |  | 16627472
TRPV4 |  | Pacsin3 | Co-immunoprecipitation | Rat | 132-144 | Mouse | 368-424 |  |  | 16627472
TRPV4 |  | TRPC1 | Fluorescence resonance energy transfer |  |  |  |  | 2:2 |  | 21871867
TRPV4 |  | TRPP1 | Atomic force microscopy(AFM) analysis |  |  |  |  | 2:2 |  | 20682256
TRPV4 |  | Tubulin | Fusion protein-pull down assay | Mouse | 718-871 | Human | Not determined |  |  | 22101010

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV4 |  | AKAP5/AKAP150 | Patch clamp |  |  | Activation | 25005230
TRPV4 |  | Calmodulin | Patch clamp |  |  | Activation | 12724311
TRPV4 |  | FYN | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | FYN | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589
TRPV4 |  | HCK | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | HCK | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589
TRPV4 |  | IP3R3 | Calcium measurement |  |  | Activation | 18378772
TRPV4 |  | IP3R3 | Calcium measurement |  |  | Activation | 18826956
TRPV4 |  | IP3R3 | Patch clamp |  |  | Activation | 18378772
TRPV4 |  | IP3R3 | Patch clamp |  |  | Activation | 18826956
TRPV4 |  | ITCH/AIP4 | Co-immunofluorescence staining |  | Decrease in plasma membrane level |  | 17110928
TRPV4 |  | LCK | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | LCK | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589
TRPV4 |  | LYN | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | LYN | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589
TRPV4 |  | MAP7 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 14517216
TRPV4 |  | MAP7 | Patch clamp |  |  | Activation | 14517216
TRPV4 |  | OS-9 | Cell surface biotinylation |  | Retain in the endoplasmic reticulum |  | 17932042
TRPV4 |  | Pacsin3 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 16627472
TRPV4 |  | Pacsin3 | Patch clamp |  |  | Inhibition | 18174177
TRPV4 |  | SRC | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | SRC | Calcium measurement |  |  | Activation | 19033444
TRPV4 |  | SRC | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589
TRPV4 |  | SRC | In vivo PTM assay | Phosphorylation (Tyr-110) |  |  | 19033444
TRPV4 |  | SRC | In vivo PTM assay | Phosphorylation (Tyr-805) |  |  | 19033444
TRPV4 |  | TRPC1 | Calcium measurement |  |  | Activation | 20093626
TRPV4 |  | TRPC1 | Calcium measurement |  |  | Activation | 20705915
TRPV4 |  | TRPC1 | Cell surface biotinylation |  | Increase in plasma membrane level |  | 20705915
TRPV4 |  | TRPC1 | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 20705915
TRPV4 |  | TRPC1 | Patch clamp |  |  | New channel creation | 25114176
TRPV4 |  | TRPP1 | Calcium measurement |  |  | New channel creation | 22518003
TRPV4 |  | TRPP1 | Patch clamp |  |  | New channel creation | 25114176
TRPV4 |  | TRPP1 | Patch clamp |  |  | New channel creation | 18695040
TRPV4 |  | UBC | In vivo PTM assay | Ubiquitination |  |  | 17110928
TRPV4 |  | UBC | In vivo PTM assay | Ubiquitination |  |  | 17932042
TRPV4 |  | YES | Calcium measurement |  |  | Activation | 12538589
TRPV4 |  | YES | In vitro PTM assay | Phosphorylation (Tyr-273) |  |  | 12538589

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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