# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TRPM4 / interactors

- At a glance
- In detail

- Basic Information
- External Database
- PPI pairs (7)
- Biological Process (5)
- Disease (1)

Name: Transient receptor potential cation channel subfamily M member 4 (TRPM4)
Official Symbol: TRPM4 provided by HGNC
Function:
Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 54795 | Q8TD43 |  |  |  | 120154 | Q8TD43 | 496 | hsa:54795 | 606936
Mouse | 68667 | Q7TN37 |  |  |  | 212980 | Q7TN37 |  | mmu:68667 |
Rat | 171143 | Q9ESQ5 |  |  |  | 251147 | Q9ESQ5 |  | rno:171143 |

PPI pairs:

14-3-3ƣ | CK1 | Calmodulin | SUR1/ABCC8 | TRPC3 | UBC | ƣENaC

Biological Process:

- GO:0002407 : dendritic cell chemotaxis
- GO:0090263 : positive regulation of canonical Wnt receptor signaling pathway
- GO:0008284 : positive regulation of cell proliferation
- GO:0016925 : protein sumoylation
- GO:0002724 : regulation of T cell cytokine production

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:606936 : Progressive familial heart block, type IB (OMIM:604559 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPM4 |  | 14-3-3ƣ | Yeast two-hybrid | Human | 1-174 | Human |  | cDNA libray |  | 25047048
TRPM4 |  | Calmodulin | Inference |  |  |  |  |  | Prediction | 15590641
TRPM4 |  | CK1 | Inference |  |  |  |  |  | Prediction | 25231975
TRPM4 |  | ƣENaC | Inference |  |  |  |  |  | Prediction | 24391909
TRPM4 |  | SUR1/ABCC8 | Inference |  |  |  |  |  | Prediction | 23255597
TRPM4 |  | TRPC3 | Yeast two-hybrid | Human | Full-length | Human | Brain | cDNA library |  | 18262493
TRPM4 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | U2OS osteosarcoma | Cell lystaes |  | 21139048
TRPM4 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | HEK293T | Cell lystaes |  | 21890473
TRPM4 |  | UBC | Affinity purification-mass spectrometry | Not used as a bait |  | Human | ?HCT116 | Cell lystaes |  | 21906983

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM4 |  | TRPC3 | Yeast two-hybrid | Human | Full-length | Yeast | Human | Full-length | Yeast | 18262493

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM4 |  | 14-3-3ƣ | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 25047048
TRPM4 |  | 14-3-3ƣ | Co-immunoprecipitation |  | HEK293T | Human | 1-174 | Human | Full-length | 25047048
TRPM4 |  | 14-3-3ƣ | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 25047048
TRPM4 |  | ƣENaC | Co-immunofluorescence staining | Rat posterior cerebral arteries |  |  |  |  |  | 24391909
TRPM4 |  | ƣENaC | Co-immunoprecipitation | Rat posterior cerebral arteries |  |  |  |  |  | 24391909
TRPM4 |  | SUR1/ABCC8 | Co-immunoprecipitation |  | COS-7 | Mouse | Full-length | Not specified | Not specified | 23255597
TRPM4 |  | SUR1/ABCC8 | Co-immunoprecipitation | Rat subarachnoid hemorrhage |  |  |  |  |  | 24114458
TRPM4 |  | SUR1/ABCC8 | Fluorescence resonance energy transfer |  | COS-7 | Mouse | Full-length | Not specified | Not specified | 23255597
TRPM4 |  | SUR1/ABCC8 | Fluorescence resonance energy transfer | COS-7 |  |  |  |  |  | 24114458
TRPM4 |  | TRPC3 | Co-immunofluorescence staining |  | HEK293T | Human | Full-length | Human | Full-length | 18262493
TRPM4 |  | TRPC3 | Co-immunoprecipitation |  | HEK293T | Human | Full-length | Human | Full-length | 18262493

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPM4 |  | 14-3-3ƣ | Co-immunoprecipitation | Human | 1-174 | Human | Full-length |  |  | 25047048
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 5-25 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 186-212 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 1076-1098 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 1146-1167 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | Calmodulin | Fusion protein-pull down assay | Human | 1095-1151 | Not specified | Not determined |  |  | 15590641
TRPM4 |  | TRPC3 | Co-immunoprecipitation | Human | 858-1188 | Human | Not determined |  |  | 18262493

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM4 |  | 14-3-3ƣ | Patch clamp |  |  | Activation | 25047048
TRPM4 |  | Calmodulin | Patch clamp |  |  | Activation | 15590641
TRPM4 |  | CK1 | In vivo PTM assay | Phosphorylation (Ser-839) |  |  | 25231975

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
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