# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# NHERF4 / TRP channels

- Basic Information
- External Database
- PPI pairs (3)
- Biological Process (6)
- Disease (0)

Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF4 (NHERF4)
Official Symbol: PDZD3 provided by HGNC
Function:
Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. Stimulates SLC9A3 activity in the presence of elevated calcium ions.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 79849 | Q86UT5 | DIP-41141N |  | MINT-1773252 | 122940 | Q86UT5 |  | hsa:79849 | 607146
Mouse | 170761 | Q99MJ6 | DIP-41892N |  | MINT-152467 | 228423 | Q99MJ6 |  | mmu:170761 |
Rat | 500986 | D4A7C5 |  |  |  | 272293 |  |  | rno:500986 |

PPI pairs:

TRPV4 | TRPV5 | TRPV6

Biological Process:

- GO:0019934 : cGMP-mediated signaling
- GO:0006811 : ion transport
- GO:0030827 : negative regulation of cGMP biosynthetic process
- GO:0007168 : receptor guanylyl cyclase signaling pathway
- GO:0009636 : response to toxin
- GO:0006833 : water transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV4 |  | NHERF4 | Inference |  |  |  |  |  | Prediction | 16565876
TRPV5 |  | NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 16565876
TRPV6 |  | NHERF4 | Yeast two-hybrid | Mouse | 575-727 | Mouse | Kidney | cDNA library |  | 17645868
TRPV6 |  | NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 16565876

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV4 |  | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876
TRPV5 |  | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876
TRPV6 |  | NHERF4 | Fusion protein-pull down assay | Mouse | 575-727 | E. coli | Mouse | Full-length | In vitro translation | 17645868
TRPV6 |  | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | NHERF4 | Co-immunofluorescence staining |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16565876
TRPV5 |  | NHERF4 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16565876
TRPV5 |  | NHERF4 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876
TRPV6 |  | NHERF4 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | NHERF4 | Fusion protein-pull down assay | Mouse | 579-617 | Mouse | Not determined |  |  | 16565876
TRPV6 |  | NHERF4 | Fusion protein-pull down assay | Mouse | 724-727 | Mouse | Not determined |  |  | 17645868

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV6 |  | NHERF4 | Patch clamp |  |  | Activation | 17645868

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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