# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# RyR2 / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (17)
- Disease (2)

Name: Ryanodine receptor 2 (RyR2)
Official Symbol: RYR2 provided by HGNC
Function:
Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 6262 | Q92736 | DIP-38325N | Q92736 | MINT-1201008 | 112174 | Q92736 |  | hsa:6262 | 180902
Mouse | 20191 | Q9ERN6 | DIP-32244N | Q9ERN6 | MINT-4129173 | 203046 | Q9ERN6 |  | mmu:20191 |
Rat | 689560 | B0LPN4 |  |  | UNK-5492549 | 604379 | B0LPN4 |  | rno:689560 |

PPI pairs:

TRPC3 | TRPP1

Biological Process:

- GO:0030509 : BMP signaling pathway
- GO:0060070 : canonical Wnt receptor signaling pathway
- GO:0060048 : cardiac muscle contraction
- GO:0003300 : cardiac muscle hypertrophy
- GO:0071313 : cellular response to caffeine
- GO:0005513 : detection of calcium ion
- GO:0003143 : embryonic heart tube morphogenesis
- GO:0008627 : intrinsic apoptotic signaling pathway in response to osmotic stress
- GO:0003220 : left ventricular cardiac muscle tissue morphogenesis
- GO:0010460 : positive regulation of heart rate
- GO:0086094 : positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction
- GO:0086029 : Purkinje myocyte to ventricular cardiac muscle cell signaling
- GO:0010881 : regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
- GO:0086005 : regulation of ventricular cardiac muscle cell action potential
- GO:0014808 : release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
- GO:0001666 : response to hypoxia
- GO:0051775 : response to redox state

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:

- OMIM:180902 : Ventricular tachycardia, catecholaminergic polymorphic, 1 (OMIM:604772 )
- OMIM:180902 : Arrhythmogenic right ventricular dysplasia 2 (OMIM:600996 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC3 |  | RyR2 | Inference |  |  |  |  |  | Prediction | 17012351
TRPP1 |  | RyR2 | Inference |  |  |  |  |  | Prediction | 17404231

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPP1 |  | RyR2 | Fusion protein-pull down assay | Human | 1-703 | MDCK lysates | Not used |  | Mouse cardiac tissue microsome | 17404231

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | RyR2 | Co-immunofluorescence staining | Rat myocyte section |  |  |  |  |  | 17012351
TRPC3 |  | RyR2 | Co-immunoprecipitation | Rat ventricle lysates |  |  |  |  |  | 17012351
TRPP1 |  | RyR2 | Co-immunoprecipitation | Mouse heart tissue |  |  |  |  |  | 17404231

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPP1 |  | RyR2 | Fusion protein-pull down assay | Human | 703-968 | Mouse | Not determined |  |  | 17404231
TRPP1 |  | RyR2 | Fusion protein-pull down assay | Human | 130-220 | Mouse | Not determined |  |  | 17404231

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.