# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# RACK1 / TRP channels

- Basic Information
- External Database
- PPI pairs (4)
- Biological Process (21)
- Disease (0)

Name: Guanine nucleotide-binding protein subunit beta-2-like 1 (RACK1)
Official Symbol: GNB2L1 provided by HGNC
Function:
Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 10399 | P63244 | DIP-46736N | P63244 | MINT-105673 | 115671 | P63244 |  | hsa:10399 | 176981
Mouse | 14694 | P68040 | DIP-49053N | P68040 | MINT-248042 | 199978 | P68040 |  | mmu:14694 |
Rat | 83427 | P63245 | DIP-37305N | P63245 | MINT-4095812 | 249704 | P63245 |  | rno:83427 |

PPI pairs:

TRPC3 | TRPM6 | TRPM7 | TRPP3

Biological Process:

- GO:0007049 : cell cycle
- GO:0007369 : gastrulation
- GO:0030308 : negative regulation of cell growth
- GO:0050765 : negative regulation of phagocytosis
- GO:0051898 : negative regulation of protein kinase B signaling cascade
- GO:0017148 : negative regulation of translation
- GO:0030178 : negative regulation of Wnt receptor signaling pathway
- GO:0043065 : positive regulation of apoptotic process
- GO:0030335 : positive regulation of cell migration
- GO:2000543 : positive regulation of gastrulation
- GO:0043547 : positive regulation of GTPase activity
- GO:2001244 : positive regulation of intrinsic apoptotic signaling pathway
- GO:0032436 : positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0032464 : positive regulation of protein homooligomerization
- GO:0001934 : positive regulation of protein phosphorylation
- GO:0051726 : regulation of cell cycle
- GO:0051302 : regulation of cell division
- GO:2000114 : regulation of establishment of cell polarity
- GO:0032880 : regulation of protein localization
- GO:0048511 : rhythmic process
- GO:0019048 : virus-host interaction

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC3 |  | RACK1 | Yeast two-hybrid | Not specified | 1-341 | Human | Embroynic kidney | cDNA library |  | 18755685
TRPM6 |  | RacK1 | Yeast two-hybrid | Human | 1075-2022 | Mouse | Kidney | cDNA library |  | 18258429
TRPM7 |  | RacK1 | Inference |  |  |  |  |  | Prediction | 18258429
TRPP3 |  | RACK1 | Yeast two-hybrid | Human | 1-291 | Human | Kidney | cDNA library |  | 22174419

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPM6 |  | RacK1 | Yeast two-hybrid | Human | 1075-2022 | Yeast | Mouse | Full-length | Yeast | 18258429
TRPM7 |  | RacK1 | Fusion protein-pull down assay | Mouse | ơ-kinase domain | E. coli | Mouse | Full-length | In vitro translation | 18258429
TRPP3 |  | RACK1 | Fusion protein-pull down assay | Human | 1-291 | HEK293 lysates | Human | Full-length | E. coli | 22174419

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | RACK1 | Co-immunofluorescence staining |  | HEK293 | Not specified | Full-length | Not used |  | 18755685
TRPC3 |  | RACK1 | Co-immunoprecipitation |  | HEK293 | Not specified | Full-length | Not used |  | 18755685
TRPC3 |  | RACK1 | Co-immunoprecipitation | Rat brain lysates |  |  |  |  |  | 18755685
TRPM6 |  | RacK1 | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 18258429
TRPM6 |  | RacK1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Not specified | Not specified | 18258429
TRPP3 |  | RACK1 | Co-immunoprecipitation |  | Xenopus oocyte | Human | Full-length | Human | Full-length | 22174419
TRPP3 |  | RACK1 | Co-immunoprecipitation |  | HEK293 | Human | Full-length | Human | Full-length | 22174419
TRPP3 |  | RACK1 | Co-immunoprecipitation |  | mIMCD-3 | Human | Full-length | Human | Full-length | 22174419
TRPP3 |  | RACK1 | Co-immunoprecipitation | Mouse kidney lysates |  |  |  |  |  | 22174419
TRPP3 |  | RACK1 | Co-immunoprecipitation | Mouse heart tissue lysates |  |  |  |  |  | 22174419
TRPP3 |  | RACK1 | Co-immunoprecipitation | Mouse brain tissue lysates |  |  |  |  |  | 22174419

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC3 |  | RACK1 | Yeast two-hybrid | Not specified | 221-342 | Human | Not determined |  |  | 18755685
TRPM6 |  | RacK1 | Fusion protein-pull down assay | Mouse | 1857-1885 | Mouse | Not determined |  |  | 18258429
TRPM7 |  | RacK1 | Fusion protein-pull down assay | Mouse | ơ-kinase domain | Mouse | Not determined |  |  | 18258429
TRPP3 |  | RACK1 | Yeast two-hybrid | Human | 1-291 | Human | 415-669 |  |  | 22174419
TRPP3 |  | RACK1 | Yeast two-hybrid | Human | 1-291 | Human | 649-951 |  |  | 22174419
TRPP3 |  | RACK1 | Yeast two-hybrid | Human | 55-291 | Human | 415-951 |  |  | 22174419
TRPP3 |  | RACK1 | Yeast two-hybrid | Human | 1696-2415 | Human | Not specified |  |  | 22174419

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPM6 |  | RacK1 | Patch clamp |  |  | Inhibition | 18258429
TRPM7 |  | RacK1 | Patch clamp |  |  | Inhibition | 18258429
TRPP3 |  | RACK1 | Calcium measurement |  |  | Inhibition | 22174419
TRPP3 |  | RACK1 | Cell surface biotinylation |  | Decrease in plasma membrane level |  | 22174419

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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