# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# TARBP2 / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (12)
- Disease (0)

Name: RISC-loading complex subunit TARBP2 {ECO:0000255|HAMAP-Rule:MF_03034} (TARBP2)
Official Symbol: TARBP2 {ECO:0000255|HAMAP-Rule:MF_03034} provided by HGNC
Function:
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. {ECO:0000269|PubMed:12475984, ECO:0000269|PubMed:15973356, ECO:0000269|PubMed:16142218, ECO:0000269|PubMed:16271387, ECO:0000269|PubMed:16357216, ECO:0000269|PubMed:16424907, ECO:0000269|PubMed:17452327, ECO:0000269|PubMed:18178619, ECO:0000269|PubMed:19219043}.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 6895 | Q15633 | DIP-29665N | Q15633 | MINT-92451 | 112758 | Q15633 |  | hsa:6895 | 605053
Mouse | 21357 | P97473 |  | P97473 |  |  | P97473 |  | mmu:21357 |
Rat | 363006 | Q3SWU0 |  |  |  |  | Q3SWU0 |  | rno:363006 |

PPI pairs:

TRPC4 | TRPC5

Biological Process:

- GO:0010467 : gene expression
- GO:0035280 : miRNA loading onto RISC involved in gene silencing by miRNA
- GO:0050689 : negative regulation of defense response to virus by host
- GO:0006469 : negative regulation of protein kinase activity
- GO:0045070 : positive regulation of viral genome replication
- GO:0031054 : pre-miRNA processing
- GO:0030422 : production of siRNA involved in RNA interference
- GO:0006357 : regulation of transcription from RNA polymerase II promoter
- GO:0006355 : regulation of transcription, DNA-templated
- GO:0006417 : regulation of translation
- GO:0046782 : regulation of viral transcription
- GO:0030423 : targeting of mRNA for destruction involved in RNA interference

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPC4 |  | TARBP2 | Yeast two-hybrid | Mouse | Not specified | Mouse | Brain | cDNA library |  | 24563462
TRPC5 |  | TARBP2 | Inference |  |  |  |  |  | Prediction | 24563462

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPC4 |  | TARBP2 | Fusion protein-pull down assay | Mouse | 625-974 | E. coli | Mouse | Full-length | HEK293 lysates | 24563462

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | TARBP2 | Co-immunoprecipitation |  | HEK293 | Mouse | Full-length | Mouse | Full-length | 24563462
TRPC5 |  | TARBP2 | Co-immunoprecipitation |  | HEK293 | Not specified | Not specified | Mouse | Full-length | 24563462

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPC4 |  | TARBP2 | Co-immunoprecipitation | Mouse | 625-974 | Mouse | 209-234 |  |  | 24563462

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.