# TRIP Database

Mammalian TRansient receptor potential channel-Interacting Protein

TRP channels

- TRPC
  - TRPC1
  - TRPC2
  - TRPC3
  - TRPC4
  - TRPC5
  - TRPC6
  - TRPC7
- TRPV
  - TRPV1
  - TRPV2
  - TRPV3
  - TRPV4
  - TRPV5
  - TRPV6
- TRPM
  - TRPM1
  - TRPM2
  - TRPM3
  - TRPM4
  - TRPM5
  - TRPM6
  - TRPM7
  - TRPM8
- TRPML
  - TRPML1
  - TRPML2
  - TRPML3
- TRPP
  - TRPP1
  - TRPP2
  - TRPP3
- TRPA
  - TRPA1

» Citation

# Rab11a / TRP channels

- Basic Information
- External Database
- PPI pairs (2)
- Biological Process (12)
- Disease (0)

Name: Ras-related protein Rab-11A (Rab11a)
Official Symbol: RAB11A provided by HGNC
Function:
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species | External DB
Name | Description
--- | ---
Entrez Gene | A database that contains genome-centered information
UniprotKB | The central hub for collection of functional information on proteins
DIP | A database that documents experimentally determined PPIs
IntAct | An open source database and software suite for modeling, storing and analyzing molecular interaction data
MINT | A database that collects experimentally verified PPIs in a binary or complex representation
BioGRID | A database of known and predicted PPIs
STRING | An online interaction repository with data
IUPHAR-DB | Integrated pharmacological and pathophysiological information on GPCR and voltage-gated & ligand-gated ion channels
KEGG | A database resource consisting of 16 main databases, categorized into systems information, genomic information, and chemical information
OMIM | A database of human genes and genetic disorders
Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM
Human | 8766 | P62491 | DIP-29138N | P62491 | MINT-1434585 | 114299 | P62491 |  | hsa:8766 | 605570
Mouse | 53869 | P62492 | DIP-32454N | P62492 | MINT-4131841 | 207498 | P62492 |  | mmu:53869 |
Rat | 81830 | P62494 | DIP-46857N | P62494 | MINT-4131864 | 249688 | P62494 |  | rno:81830 |

PPI pairs:

TRPV5 | TRPV6

Biological Process:

- GO:0007049 : cell cycle
- GO:0000910 : cytokinesis
- GO:0032402 : melanosome transport
- GO:0031175 : neuron projection development
- GO:0048227 : plasma membrane to endosome transport
- GO:0072659 : protein localization to plasma membrane
- GO:0015031 : protein transport
- GO:0048169 : regulation of long-term neuronal synaptic plasticity
- GO:0051223 : regulation of protein transport
- GO:0007264 : small GTPase mediated signal transduction
- GO:0055085 : transmembrane transport
- GO:0006833 : water transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease: No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Screening
| Experimental screening | Non-experimental screening | Reference
--- | --- | --- | ---
TRP channel construct | Interactor source
TRP channel |  | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type
TRPV5 |  | Rab11a | Inference |  |  |  |  |  | Prediction | 16354700
TRPV6 |  | Rab11a | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library |  | 16354700

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vitro validation
| Assay with recombinant proteins | Reference
--- | --- | ---
TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system
TRPV5 |  | Rab11a | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 16354700
TRPV6 |  | Rab11a | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 16354700

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Validation: In vivo validation
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference
--- | --- | --- | ---
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | Rab11a | Co-immunofluorescence staining |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700
TRPV5 |  | Rab11a | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16354700
TRPV5 |  | Rab11a | Co-immunoprecipitation |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700
TRPV6 |  | Rab11a | Co-immunofluorescence staining | Mouse kidney section |  |  |  |  |  | 16354700
TRPV6 |  | Rab11a | Co-immunofluorescence staining |  | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Characterization
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference
--- | --- | --- | --- | ---
TRP channel | Interactor
TRP channel |  | Interactor | Method | Species | Region | Species | Region
TRPV5 |  | Rab11a | Fusion protein-pull down assay | Mouse | 596-600 | Mouse | Not determined |  |  | 16354700

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening Validation: In vitro validation Validation: In vivo validation Characterization Functional consequence | top

Functional consequence
TRP channel |  | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference
--- | --- | --- | --- | --- | --- | --- | ---
TRPV5 |  | Rab11a | Calcium measurement |  |  | Activation | 16354700
TRPV5 |  | Rab11a | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 16354700
TRPV6 |  | Rab11a | Calcium measurement |  |  | Activation | 16354700
TRPV6 |  | Rab11a | Co-immunofluorescence staining |  | Increase in plasma membrane level |  | 16354700

(: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Name Email Title* Comment* TRP
/ Interactor
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